Porcine gamma-synuclein: molecular cloning, expression analysis, chromosomal localization and functional expression

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Porcine gamma-synuclein: molecular cloning, expression analysis, chromosomal localization and functional expression. / Frandsen, Pernille Munk; Madsen, Lone Bruhn; Bendixen, Christian; Larsen, Knud.

I: Molecular Biology Reports, Bind 36, Nr. 5, 2009, s. 971-979.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Frandsen, Pernille Munk ; Madsen, Lone Bruhn ; Bendixen, Christian ; Larsen, Knud. / Porcine gamma-synuclein: molecular cloning, expression analysis, chromosomal localization and functional expression. I: Molecular Biology Reports. 2009 ; Bind 36, Nr. 5. s. 971-979.

Bibtex

@article{f432f3a0a71311dda608000ea68e967b,
title = "Porcine gamma-synuclein: molecular cloning, expression analysis, chromosomal localization and functional expression",
abstract = "The γ-synuclein protein is involved in breast carcinogenesis and has also been implicated in other forms of cancer and in ocular diseases. Furthermore, γ-synuclein is believed to have a role in certain neurodegenerative diseases, such as Parkinson's disease and Alzheimer's disease. This work reports the cloning and characterization of the porcine (Sus scrofa) γ-synuclein cDNA (SNCG). The SNCG cDNA was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) using oligonucleotide primers derived from in silico sequences. The porcine SNCG cDNA codes for a protein of 126 amino acids which shows a high similarity to bovine (90{\%}), human (87{\%}) and mouse (83{\%}) γ-synuclein. A genomic clone containing the entire porcine SNCG gene was isolated and its genomic organization determined. The gene is composed of five exons, the general structure being observed to be very similar to that of the human SNCG gene. Expression analysis by quantitative real-time RT-PCR revealed the presence of SNCG transcripts in all examined organs and tissues. Differential expression was observed, with very high levels of SNCG mRNA in fat tissue and high expression levels in spleen, cerebellum, frontal cortex and pituitary gland. Expression analysis also showed that porcine SNCG transcripts could be detected in different brain regions during early stages of embryo development. The porcine SNCG orthologue was mapped to chromosome 14q25-q29. The distribution of recombinant porcine γ-synuclein was studied in three different transfected cell lines and the protein was found to be predominantly localized in the cytoplasm",
keywords = "Animal models, Brain, Embryogenesis, γ-synuclein, Pig, SNP",
author = "Frandsen, {Pernille Munk} and Madsen, {Lone Bruhn} and Christian Bendixen and Knud Larsen",
year = "2009",
doi = "10.1007/s11033-008-9270-z",
language = "English",
volume = "36",
pages = "971--979",
journal = "Molecular Biology Reports",
issn = "0301-4851",
publisher = "Springer",
number = "5",

}

RIS

TY - JOUR

T1 - Porcine gamma-synuclein: molecular cloning, expression analysis, chromosomal localization and functional expression

AU - Frandsen, Pernille Munk

AU - Madsen, Lone Bruhn

AU - Bendixen, Christian

AU - Larsen, Knud

PY - 2009

Y1 - 2009

N2 - The γ-synuclein protein is involved in breast carcinogenesis and has also been implicated in other forms of cancer and in ocular diseases. Furthermore, γ-synuclein is believed to have a role in certain neurodegenerative diseases, such as Parkinson's disease and Alzheimer's disease. This work reports the cloning and characterization of the porcine (Sus scrofa) γ-synuclein cDNA (SNCG). The SNCG cDNA was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) using oligonucleotide primers derived from in silico sequences. The porcine SNCG cDNA codes for a protein of 126 amino acids which shows a high similarity to bovine (90%), human (87%) and mouse (83%) γ-synuclein. A genomic clone containing the entire porcine SNCG gene was isolated and its genomic organization determined. The gene is composed of five exons, the general structure being observed to be very similar to that of the human SNCG gene. Expression analysis by quantitative real-time RT-PCR revealed the presence of SNCG transcripts in all examined organs and tissues. Differential expression was observed, with very high levels of SNCG mRNA in fat tissue and high expression levels in spleen, cerebellum, frontal cortex and pituitary gland. Expression analysis also showed that porcine SNCG transcripts could be detected in different brain regions during early stages of embryo development. The porcine SNCG orthologue was mapped to chromosome 14q25-q29. The distribution of recombinant porcine γ-synuclein was studied in three different transfected cell lines and the protein was found to be predominantly localized in the cytoplasm

AB - The γ-synuclein protein is involved in breast carcinogenesis and has also been implicated in other forms of cancer and in ocular diseases. Furthermore, γ-synuclein is believed to have a role in certain neurodegenerative diseases, such as Parkinson's disease and Alzheimer's disease. This work reports the cloning and characterization of the porcine (Sus scrofa) γ-synuclein cDNA (SNCG). The SNCG cDNA was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) using oligonucleotide primers derived from in silico sequences. The porcine SNCG cDNA codes for a protein of 126 amino acids which shows a high similarity to bovine (90%), human (87%) and mouse (83%) γ-synuclein. A genomic clone containing the entire porcine SNCG gene was isolated and its genomic organization determined. The gene is composed of five exons, the general structure being observed to be very similar to that of the human SNCG gene. Expression analysis by quantitative real-time RT-PCR revealed the presence of SNCG transcripts in all examined organs and tissues. Differential expression was observed, with very high levels of SNCG mRNA in fat tissue and high expression levels in spleen, cerebellum, frontal cortex and pituitary gland. Expression analysis also showed that porcine SNCG transcripts could be detected in different brain regions during early stages of embryo development. The porcine SNCG orthologue was mapped to chromosome 14q25-q29. The distribution of recombinant porcine γ-synuclein was studied in three different transfected cell lines and the protein was found to be predominantly localized in the cytoplasm

KW - Animal models

KW - Brain

KW - Embryogenesis

KW - γ-synuclein

KW - Pig

KW - SNP

U2 - 10.1007/s11033-008-9270-z

DO - 10.1007/s11033-008-9270-z

M3 - Journal article

C2 - 18461469

VL - 36

SP - 971

EP - 979

JO - Molecular Biology Reports

JF - Molecular Biology Reports

SN - 0301-4851

IS - 5

ER -