Porcine dorfin: molecular cloning of the RNF19 gene, sequence comparison, mapping and expression analysis

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

Porcine dorfin : molecular cloning of the RNF19 gene, sequence comparison, mapping and expression analysis. / Larsen, Knud Erik; Madsen, Lone Bruhn; Bendixen, Christian.

I: Molecular Biology Reports, Bind 39, Nr. 12, 2012, s. 10053-10062.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

APA

CBE

MLA

Vancouver

Author

Larsen, Knud Erik ; Madsen, Lone Bruhn ; Bendixen, Christian. / Porcine dorfin : molecular cloning of the RNF19 gene, sequence comparison, mapping and expression analysis. I: Molecular Biology Reports. 2012 ; Bind 39, Nr. 12. s. 10053-10062.

Bibtex

@article{ff3668e52a48469eb5569e3b9c5b95a5,
title = "Porcine dorfin: molecular cloning of the RNF19 gene, sequence comparison, mapping and expression analysis",
abstract = "Dorfin, encoded by the RNF19 gene, is a protein containing two RING finger motifs. Dorfin functions as an E3 ubiquitin ligase that interacts with UBE2L3/UBCH7 and UBE2E2/UBCH8, but not other ubiquitin-conjugating enzymes. Dorfin is found expressed in Lewy bodies, neuronal protein inclusions occurring in Parkinson's disease brains. This work reports the cloning and analysis of the porcine (Sus scrofa) homologue of dorfin. The RNF19 cDNA encoding dorfin was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) using oligonucleotide primers derived from in silico sequences. The porcine RNF19 cDNA codes for a protein of 838 amino acids which shows a very high similarity to human (97 %) and mouse (93 %) dorfin. The genomic organization of the porcine RNF19 gene is very similar to its human counterpart. Expression analysis by RT-PCR demonstrated that the porcine RNF19 transcript was observed in all organs and tissues examined, although differentially expressed. The highest expression of RNF19 mRNA was observed in cerebellum, heart, frontal cortex and muscle. RNF19 transcript was detected as early as 60 days of gestation in many different brain areas. Radiation hybrid mapping data indicate that the porcine RNF19 gene maps to chromosome 4 (4p11-p12). This particular map location is fully consistent with the currently known conservation of genome organization between human and pig and provides further confirmation that we have characterized the porcine homologue of the human RNF19.",
keywords = "Brain, cDNA, Dorfin, Developing embryos, Pig, RNF19",
author = "Larsen, {Knud Erik} and Madsen, {Lone Bruhn} and Christian Bendixen",
year = "2012",
doi = "10.1007/s11033-012-1874-7",
language = "English",
volume = "39",
pages = "10053--10062",
journal = "Molecular Biology Reports",
issn = "0301-4851",
publisher = "Springer",
number = "12",

}

RIS

TY - JOUR

T1 - Porcine dorfin

T2 - molecular cloning of the RNF19 gene, sequence comparison, mapping and expression analysis

AU - Larsen, Knud Erik

AU - Madsen, Lone Bruhn

AU - Bendixen, Christian

PY - 2012

Y1 - 2012

N2 - Dorfin, encoded by the RNF19 gene, is a protein containing two RING finger motifs. Dorfin functions as an E3 ubiquitin ligase that interacts with UBE2L3/UBCH7 and UBE2E2/UBCH8, but not other ubiquitin-conjugating enzymes. Dorfin is found expressed in Lewy bodies, neuronal protein inclusions occurring in Parkinson's disease brains. This work reports the cloning and analysis of the porcine (Sus scrofa) homologue of dorfin. The RNF19 cDNA encoding dorfin was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) using oligonucleotide primers derived from in silico sequences. The porcine RNF19 cDNA codes for a protein of 838 amino acids which shows a very high similarity to human (97 %) and mouse (93 %) dorfin. The genomic organization of the porcine RNF19 gene is very similar to its human counterpart. Expression analysis by RT-PCR demonstrated that the porcine RNF19 transcript was observed in all organs and tissues examined, although differentially expressed. The highest expression of RNF19 mRNA was observed in cerebellum, heart, frontal cortex and muscle. RNF19 transcript was detected as early as 60 days of gestation in many different brain areas. Radiation hybrid mapping data indicate that the porcine RNF19 gene maps to chromosome 4 (4p11-p12). This particular map location is fully consistent with the currently known conservation of genome organization between human and pig and provides further confirmation that we have characterized the porcine homologue of the human RNF19.

AB - Dorfin, encoded by the RNF19 gene, is a protein containing two RING finger motifs. Dorfin functions as an E3 ubiquitin ligase that interacts with UBE2L3/UBCH7 and UBE2E2/UBCH8, but not other ubiquitin-conjugating enzymes. Dorfin is found expressed in Lewy bodies, neuronal protein inclusions occurring in Parkinson's disease brains. This work reports the cloning and analysis of the porcine (Sus scrofa) homologue of dorfin. The RNF19 cDNA encoding dorfin was amplified by reverse transcriptase polymerase chain reaction (RT-PCR) using oligonucleotide primers derived from in silico sequences. The porcine RNF19 cDNA codes for a protein of 838 amino acids which shows a very high similarity to human (97 %) and mouse (93 %) dorfin. The genomic organization of the porcine RNF19 gene is very similar to its human counterpart. Expression analysis by RT-PCR demonstrated that the porcine RNF19 transcript was observed in all organs and tissues examined, although differentially expressed. The highest expression of RNF19 mRNA was observed in cerebellum, heart, frontal cortex and muscle. RNF19 transcript was detected as early as 60 days of gestation in many different brain areas. Radiation hybrid mapping data indicate that the porcine RNF19 gene maps to chromosome 4 (4p11-p12). This particular map location is fully consistent with the currently known conservation of genome organization between human and pig and provides further confirmation that we have characterized the porcine homologue of the human RNF19.

KW - Brain

KW - cDNA

KW - Dorfin

KW - Developing embryos

KW - Pig

KW - RNF19

U2 - 10.1007/s11033-012-1874-7

DO - 10.1007/s11033-012-1874-7

M3 - Journal article

C2 - 22760261

VL - 39

SP - 10053

EP - 10062

JO - Molecular Biology Reports

JF - Molecular Biology Reports

SN - 0301-4851

IS - 12

ER -