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Polymer–Lipid Hybrid Vesicles and Their Interaction with HepG2 Cells

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Polymer–Lipid Hybrid Vesicles and Their Interaction with HepG2 Cells. / Brodszkij, Edit; Westensee, Isabella N.; Bertelsen, Mathias; Gal, Noga; Boesen, Thomas; Städler, Brigitte.

I: Small, Bind 16, Nr. 27, 1906493, 07.2020.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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@article{9faf88c9ff0a47f5929a25bb9960a701,
title = "Polymer–Lipid Hybrid Vesicles and Their Interaction with HepG2 Cells",
abstract = "Polymer–lipid hybrid vesicles are an emerging type of nano-assemblies that show potential as artificial organelles among others. Phospholipids and poly(cholesteryl methacrylate)-block-poly(methionine methacryloyloxyethyl ester (METMA)—random–2-carboxyethyl acrylate (CEA)) labeled with a F{\"o}rster resonance energy transfer (FRET) reporter pair are used for the assembly of small and giant hybrid vesicles with homogenous distribution of both building blocks in the membrane as confirmed by the FRET effect. These hybrid vesicles have no inherent cytotoxicity when incubated with HepG2 cells up to 1.1 × 1011 hybrid vesicles per mL, and they are internalized by the cells. In contrast to the fluorescent signal originating from the block copolymer, the fluorescent signal coming from the lipids is barely detectable in cells incubated with hybrid vesicles for 6 h followed by 24 h in cell media, suggesting that the two building blocks have a different intracellular fate. These findings provide important insight into the design criteria of artificial organelles with potential structural integrity.",
keywords = "block copolymers, FRET effect, HepG2 cells, hybrid vesicles, phospholipids, PHASE-SEPARATION, NANOREACTORS, ENZYMES, NANOSCALE, ARTIFICIAL ORGANELLES, HOLLOW SILICA NANOSPHERES",
author = "Edit Brodszkij and Westensee, {Isabella N.} and Mathias Bertelsen and Noga Gal and Thomas Boesen and Brigitte St{\"a}dler",
year = "2020",
month = jul,
doi = "10.1002/smll.201906493",
language = "English",
volume = "16",
journal = "Small",
issn = "1613-6810",
publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",
number = "27",

}

RIS

TY - JOUR

T1 - Polymer–Lipid Hybrid Vesicles and Their Interaction with HepG2 Cells

AU - Brodszkij, Edit

AU - Westensee, Isabella N.

AU - Bertelsen, Mathias

AU - Gal, Noga

AU - Boesen, Thomas

AU - Städler, Brigitte

PY - 2020/7

Y1 - 2020/7

N2 - Polymer–lipid hybrid vesicles are an emerging type of nano-assemblies that show potential as artificial organelles among others. Phospholipids and poly(cholesteryl methacrylate)-block-poly(methionine methacryloyloxyethyl ester (METMA)—random–2-carboxyethyl acrylate (CEA)) labeled with a Förster resonance energy transfer (FRET) reporter pair are used for the assembly of small and giant hybrid vesicles with homogenous distribution of both building blocks in the membrane as confirmed by the FRET effect. These hybrid vesicles have no inherent cytotoxicity when incubated with HepG2 cells up to 1.1 × 1011 hybrid vesicles per mL, and they are internalized by the cells. In contrast to the fluorescent signal originating from the block copolymer, the fluorescent signal coming from the lipids is barely detectable in cells incubated with hybrid vesicles for 6 h followed by 24 h in cell media, suggesting that the two building blocks have a different intracellular fate. These findings provide important insight into the design criteria of artificial organelles with potential structural integrity.

AB - Polymer–lipid hybrid vesicles are an emerging type of nano-assemblies that show potential as artificial organelles among others. Phospholipids and poly(cholesteryl methacrylate)-block-poly(methionine methacryloyloxyethyl ester (METMA)—random–2-carboxyethyl acrylate (CEA)) labeled with a Förster resonance energy transfer (FRET) reporter pair are used for the assembly of small and giant hybrid vesicles with homogenous distribution of both building blocks in the membrane as confirmed by the FRET effect. These hybrid vesicles have no inherent cytotoxicity when incubated with HepG2 cells up to 1.1 × 1011 hybrid vesicles per mL, and they are internalized by the cells. In contrast to the fluorescent signal originating from the block copolymer, the fluorescent signal coming from the lipids is barely detectable in cells incubated with hybrid vesicles for 6 h followed by 24 h in cell media, suggesting that the two building blocks have a different intracellular fate. These findings provide important insight into the design criteria of artificial organelles with potential structural integrity.

KW - block copolymers

KW - FRET effect

KW - HepG2 cells

KW - hybrid vesicles

KW - phospholipids

KW - PHASE-SEPARATION

KW - NANOREACTORS

KW - ENZYMES

KW - NANOSCALE

KW - ARTIFICIAL ORGANELLES

KW - HOLLOW SILICA NANOSPHERES

UR - http://www.scopus.com/inward/record.url?scp=85085529093&partnerID=8YFLogxK

U2 - 10.1002/smll.201906493

DO - 10.1002/smll.201906493

M3 - Journal article

C2 - 32468702

AN - SCOPUS:85085529093

VL - 16

JO - Small

JF - Small

SN - 1613-6810

IS - 27

M1 - 1906493

ER -