Polymer–Lipid Hybrid Vesicles and Their Interaction with HepG2 Cells

Edit Brodszkij; Isabella N. Westensee; Mathias Bertelsen; Noga Gal; Thomas Boesen; Brigitte Städler

doi:10.1002/smll.201906493

Polymer–Lipid Hybrid Vesicles and Their Interaction with HepG2 Cells

Edit Brodszkij, Isabella N. Westensee, Mathias Bertelsen, Noga Gal, Thomas Boesen, Brigitte Städler^*

^*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

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Abstract

Polymer–lipid hybrid vesicles are an emerging type of nano-assemblies that show potential as artificial organelles among others. Phospholipids and poly(cholesteryl methacrylate)-block-poly(methionine methacryloyloxyethyl ester (METMA)—random–2-carboxyethyl acrylate (CEA)) labeled with a Förster resonance energy transfer (FRET) reporter pair are used for the assembly of small and giant hybrid vesicles with homogenous distribution of both building blocks in the membrane as confirmed by the FRET effect. These hybrid vesicles have no inherent cytotoxicity when incubated with HepG2 cells up to 1.1 × 10¹¹ hybrid vesicles per mL, and they are internalized by the cells. In contrast to the fluorescent signal originating from the block copolymer, the fluorescent signal coming from the lipids is barely detectable in cells incubated with hybrid vesicles for 6 h followed by 24 h in cell media, suggesting that the two building blocks have a different intracellular fate. These findings provide important insight into the design criteria of artificial organelles with potential structural integrity.

Originalsprog	Engelsk
Artikelnummer	1906493
Tidsskrift	Small
Vol/bind	16
Nummer	27
Antal sider	9
ISSN	1613-6810
DOI	https://doi.org/10.1002/smll.201906493
Status	Udgivet - jul. 2020

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10.1002/smll.201906493

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Citationsformater

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abstract = "Polymer–lipid hybrid vesicles are an emerging type of nano-assemblies that show potential as artificial organelles among others. Phospholipids and poly(cholesteryl methacrylate)-block-poly(methionine methacryloyloxyethyl ester (METMA)—random–2-carboxyethyl acrylate (CEA)) labeled with a F{\"o}rster resonance energy transfer (FRET) reporter pair are used for the assembly of small and giant hybrid vesicles with homogenous distribution of both building blocks in the membrane as confirmed by the FRET effect. These hybrid vesicles have no inherent cytotoxicity when incubated with HepG2 cells up to 1.1 × 1011 hybrid vesicles per mL, and they are internalized by the cells. In contrast to the fluorescent signal originating from the block copolymer, the fluorescent signal coming from the lipids is barely detectable in cells incubated with hybrid vesicles for 6 h followed by 24 h in cell media, suggesting that the two building blocks have a different intracellular fate. These findings provide important insight into the design criteria of artificial organelles with potential structural integrity.",

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AU - Brodszkij, Edit

AU - Westensee, Isabella N.

AU - Bertelsen, Mathias

AU - Gal, Noga

AU - Boesen, Thomas

AU - Städler, Brigitte

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N2 - Polymer–lipid hybrid vesicles are an emerging type of nano-assemblies that show potential as artificial organelles among others. Phospholipids and poly(cholesteryl methacrylate)-block-poly(methionine methacryloyloxyethyl ester (METMA)—random–2-carboxyethyl acrylate (CEA)) labeled with a Förster resonance energy transfer (FRET) reporter pair are used for the assembly of small and giant hybrid vesicles with homogenous distribution of both building blocks in the membrane as confirmed by the FRET effect. These hybrid vesicles have no inherent cytotoxicity when incubated with HepG2 cells up to 1.1 × 1011 hybrid vesicles per mL, and they are internalized by the cells. In contrast to the fluorescent signal originating from the block copolymer, the fluorescent signal coming from the lipids is barely detectable in cells incubated with hybrid vesicles for 6 h followed by 24 h in cell media, suggesting that the two building blocks have a different intracellular fate. These findings provide important insight into the design criteria of artificial organelles with potential structural integrity.

AB - Polymer–lipid hybrid vesicles are an emerging type of nano-assemblies that show potential as artificial organelles among others. Phospholipids and poly(cholesteryl methacrylate)-block-poly(methionine methacryloyloxyethyl ester (METMA)—random–2-carboxyethyl acrylate (CEA)) labeled with a Förster resonance energy transfer (FRET) reporter pair are used for the assembly of small and giant hybrid vesicles with homogenous distribution of both building blocks in the membrane as confirmed by the FRET effect. These hybrid vesicles have no inherent cytotoxicity when incubated with HepG2 cells up to 1.1 × 1011 hybrid vesicles per mL, and they are internalized by the cells. In contrast to the fluorescent signal originating from the block copolymer, the fluorescent signal coming from the lipids is barely detectable in cells incubated with hybrid vesicles for 6 h followed by 24 h in cell media, suggesting that the two building blocks have a different intracellular fate. These findings provide important insight into the design criteria of artificial organelles with potential structural integrity.

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KW - phospholipids

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KW - NANOREACTORS

KW - ENZYMES

KW - NANOSCALE

KW - ARTIFICIAL ORGANELLES

KW - HOLLOW SILICA NANOSPHERES

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SN - 1613-6810

VL - 16

JO - Small

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ER -

Polymer–Lipid Hybrid Vesicles and Their Interaction with HepG2 Cells

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