Podosome formation and development in monocytes restricted by the nanoscale spatial distribution of ICAM1

TY - JOUR

T1 - Podosome formation and development in monocytes restricted by the nanoscale spatial distribution of ICAM1

AU - Andersen, Andreas Søe

AU - Aslan, Hüsnü

AU - Dong, Mingdong

AU - Jiang, Xingyu

AU - Sutherland, Duncan

PY - 2016/3/9

Y1 - 2016/3/9

N2 - We studied podosome formation and development in activated monocytes (THP1) at ICAM1 (intercellular adhesion molecule 1) nanopatterns of circular and ring-shaped domains and show that cellular binding to a preclustered ICAM1 nanopattern requires ligand patches of at least 200 nm (corresponding to 14 or more integrins). Podosome-like adhesion formation depends on the structure of the ligand pattern under the developing podosome with larger single domains promoting adhesion in a single patch and multiple smaller domains allowing podosome formation by integration of at least 2 smaller domains on either side of the podosome core. Maturation to rosette structures and recruitment of proteases were only observed with macroscopic ICAM1 presentation.

AB - We studied podosome formation and development in activated monocytes (THP1) at ICAM1 (intercellular adhesion molecule 1) nanopatterns of circular and ring-shaped domains and show that cellular binding to a preclustered ICAM1 nanopattern requires ligand patches of at least 200 nm (corresponding to 14 or more integrins). Podosome-like adhesion formation depends on the structure of the ligand pattern under the developing podosome with larger single domains promoting adhesion in a single patch and multiple smaller domains allowing podosome formation by integration of at least 2 smaller domains on either side of the podosome core. Maturation to rosette structures and recruitment of proteases were only observed with macroscopic ICAM1 presentation.

KW - ICAM1

KW - THP1

KW - adhesion

KW - nanopattern

KW - podosome

UR - http://www.scopus.com/inward/record.url?scp=84960532284&partnerID=8YFLogxK

U2 - 10.1021/acs.nanolett.6b00519

DO - 10.1021/acs.nanolett.6b00519

M3 - Letter

C2 - 26861163

SN - 1530-6984

VL - 16

SP - 2114

EP - 2121

JO - Nano Letters

JF - Nano Letters

IS - 3

ER -