Plant-derived molecules α-boswellic acid acetate, praeruptorin-A and salvianolic acid-B have age-related differential effects in young and senescent human fibroblasts in vitro

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DOI

  • Anna Lewinska, Department of Biochemistry and Cell Biology, University of Rzeszow., Polen
  • Lakshman Sodagam
  • ,
  • Dominika Bloniarz, Department of Biotechnology, University of Rzeszow, Polen
  • Karsten Siems, AnalytiCon Discovery GmbH, Tyskland
  • Maciej Wnuk, Department of Genetics, University of Rzeszow, Polen
  • Suresh Rattan

Testing and screening of plant-derived molecules on normal human cells in vitro is a widely used approach for discovering their eventual health beneficial effects for human ageing and longevity. As little is known about age-associated differential effects of such molecules, here we report that young (<25% replicative lifespan completed) and near-senescent (>90% replicative lifespan completed) human skin fibroblasts exposed for 1-15 days to a wide range of concentrations (0.1-100 μM) of the three selected phytochemicals, namely α-boswellic acid acetate (ABC), praeruptorin-A (PTA), and salvianolic acid-B (SAB) had age-related differential effects. The parameters studied were the metabolic activity (MTT assay), cellular morphological phenotype, one-step growth characteristics, expression of genes involved in the cell cycle regulation and cytokine network genes, protein levels of p53, cytosolic superoxide dismutase (SOD1) and microtubule-associated protein 1A/1B-light chain 3 (LC3), and the extent of protein carbonylation and protein aggregation as a sign of oxidative stress. All three compounds showed biphasic hormetic dose response by stimulating cell growth, survival and metabolic activity at low doses (up to 1 μM), while showing inhibitory effects at high doses (>10 μM). Furthermore, the response of early passage young cells was different from that of the late passage near-senescent cells, especially with respect to the expression of cell cycle-related and inflammation-related genes. Such studies have importance with respect to the use of low doses of such molecules as health-promoting and/or ageing-interventions through the phenomenon of hormesis.

OriginalsprogEngelsk
Artikelnummer141
TidsskriftMolecules
Vol/bind25
Nummer1
Antal sider14
ISSN1420-3049
DOI
StatusUdgivet - 2020

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