TY - JOUR
T1 - Pseudomonas hormoni sp. nov., a plant hormone producing bacterium isolated from Arctic grass, Ellesmere Island, Canada
AU - Sorty, Ajay Madhusudan
AU - Zervas, Athanasios
AU - García de Salamone, Inés Eugenia
AU - Nelson, Louise M.
AU - Stougaard, Peter
N1 - Publisher Copyright:
© 2023 The Authors.
PY - 2023/10
Y1 - 2023/10
N2 - Bacterial strain G20-18T was previously isolated from the rhizosphere of an Arctic grass on Ellesmere Island, Canada and was characterized and described as Pseudomonas fluorescens. However, new polyphasic analyses coupled with phenotypic, phylogenetic and genomic analyses reported here demonstrate that the affiliation to the species P. fluorescens was incorrect. The strain is Gram-stain-negative, rod-shaped, aerobic and displays growth at 5–25 °C (optimum, 20–25 °C), at pH 5–9 (optimum, pH 6–7) and with 0–4 % NaCl (optimum, 2 % NaCl). The major fatty acids are C16: 0 (35.6 %), C17: 0 cyclo ω7c (26.3 %) and summed feature C18: 1 /C18: 1 ω7c (13.6 %). The respiratory quinones were determined to be Q9 (93.5 %) and Q8 (6.5 %) and the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Strain G20-18T was shown to syn-thesize cytokinin and auxin plant hormones and to produce 1-aminocyclopropane-1-carboxylate deaminase. The DNA G+C content was determined to be 59.1 mol%. Phylogenetic analysis based on the 16S rRNA gene and multilocus sequence analysis (concatenated 16S rRNA, gyrB, rpoB and rpoD sequences) showed that G20-18T was affiliated with the Pseudomonas mandelii subgroup within the genus Pseudomonas. Comparisons of the G20-18T genome sequence and related Pseudomonas type strain sequences showed an average nucleotide identity value of ≤93.6 % and a digital DNA–DNA hybridization value of less than 54.4 % relatedness. The phenotypic, phylogenetic and genomic data support the hypothesis that strain G20-18T represents a novel species of the genus Pseudomonas. As strain G20-18T produces or modifies hormones, the name Pseudomonas hormoni sp. nov. is proposed. The type strain is G20-18T (=LMG 33086T=NCIMB 15469T).
AB - Bacterial strain G20-18T was previously isolated from the rhizosphere of an Arctic grass on Ellesmere Island, Canada and was characterized and described as Pseudomonas fluorescens. However, new polyphasic analyses coupled with phenotypic, phylogenetic and genomic analyses reported here demonstrate that the affiliation to the species P. fluorescens was incorrect. The strain is Gram-stain-negative, rod-shaped, aerobic and displays growth at 5–25 °C (optimum, 20–25 °C), at pH 5–9 (optimum, pH 6–7) and with 0–4 % NaCl (optimum, 2 % NaCl). The major fatty acids are C16: 0 (35.6 %), C17: 0 cyclo ω7c (26.3 %) and summed feature C18: 1 /C18: 1 ω7c (13.6 %). The respiratory quinones were determined to be Q9 (93.5 %) and Q8 (6.5 %) and the major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Strain G20-18T was shown to syn-thesize cytokinin and auxin plant hormones and to produce 1-aminocyclopropane-1-carboxylate deaminase. The DNA G+C content was determined to be 59.1 mol%. Phylogenetic analysis based on the 16S rRNA gene and multilocus sequence analysis (concatenated 16S rRNA, gyrB, rpoB and rpoD sequences) showed that G20-18T was affiliated with the Pseudomonas mandelii subgroup within the genus Pseudomonas. Comparisons of the G20-18T genome sequence and related Pseudomonas type strain sequences showed an average nucleotide identity value of ≤93.6 % and a digital DNA–DNA hybridization value of less than 54.4 % relatedness. The phenotypic, phylogenetic and genomic data support the hypothesis that strain G20-18T represents a novel species of the genus Pseudomonas. As strain G20-18T produces or modifies hormones, the name Pseudomonas hormoni sp. nov. is proposed. The type strain is G20-18T (=LMG 33086T=NCIMB 15469T).
KW - ACC deaminase
KW - auxin
KW - cytokinin
KW - IAA
KW - phylogenomics
KW - plant hormones
KW - Pseudomonas mandelii subgroup
KW - Genes, Bacterial
KW - Sodium Chloride
KW - RNA, Ribosomal, 16S/genetics
KW - Poaceae
KW - Phospholipids/chemistry
KW - Phylogeny
KW - Pseudomonas
KW - Sequence Analysis, DNA
KW - Plant Growth Regulators
KW - Base Composition
KW - Bacterial Typing Techniques
KW - Fatty Acids/chemistry
KW - DNA, Bacterial/genetics
UR - http://www.scopus.com/inward/record.url?scp=85175174885&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.006119
DO - 10.1099/ijsem.0.006119
M3 - Journal article
C2 - 37889848
AN - SCOPUS:85175174885
SN - 1466-5026
VL - 73
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 10
M1 - 006119
ER -