The present study investigated the feasibility of using pilot-scale anaerobic filter to treat by-productliquid (brown juice, BJ) from grass protein extraction in a green biorefinery, and produce biogas viaanaerobic digestion without co-digestion substrate. Prior to feed into the un-heated anaerobic filterreactor, the BJ was warmed up to 55−60◦C in order to maintain the digestion temperature. The influenceof retention time and feeding frequencies were tested in order to optimize the overall performance. Thestudy demonstrated that anaerobic filter is feasible for converting BJ to biogas. The specific CH productionreached 230 mL gCOD−1d−1while up to 80 % of influent COD was removed within a hydraulic retentiontime of 5.5 days. In addition, higher feeding frequencies enhanced the process stability as it narrowedthe temperature variation and decreased the instant impact on buffer capacity.