Phasing the 30S ribosomal subunit structure

TY - JOUR

T1 - Phasing the 30S ribosomal subunit structure

AU - Brodersen, Ditlev Egeskov

AU - Clemons, Jr., W. M.

AU - Carter, A. P.

AU - Wimberly, B. T.

AU - Ramakrishnan, V.

PY - 2003

Y1 - 2003

N2 - The methods involved in determining the 850 kDa structure of the 30S ribosomal subunit from Thermus thermophilus were in many ways identical to those that are generally used in standard protein crystallography. This paper reviews and analyses the methods that can be used in phasing such large structures and shows that the anomalous signal collected from heavy-atom compounds bound to the RNA is both necessary and sufficient for ab initio structure determination at high resolution. In addition, measures to counter problems with non-isomorphism and radiation decay are described.

AB - The methods involved in determining the 850 kDa structure of the 30S ribosomal subunit from Thermus thermophilus were in many ways identical to those that are generally used in standard protein crystallography. This paper reviews and analyses the methods that can be used in phasing such large structures and shows that the anomalous signal collected from heavy-atom compounds bound to the RNA is both necessary and sufficient for ab initio structure determination at high resolution. In addition, measures to counter problems with non-isomorphism and radiation decay are described.

M3 - Journal article

SN - 0907-4449

VL - D59

SP - 2044

EP - 2050

JO - Acta Crystallographica. Section D: Biological Crystallography

JF - Acta Crystallographica. Section D: Biological Crystallography

ER -