Pericyte modulation by a functional antibody obtained by a novel single cell selection strategy

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Pericyte modulation by a functional antibody obtained by a novel single cell selection strategy. / Just, Jesper; Lykkemark, Simon; Nielsen, Charlotte Høgsbjerg; Roshenas, Ali Reza; Drasbek, Kim Ryun; Petersen, Steen Vang; Bek, Toke; Kristensen, Peter.

I: Microcirculation, Bind 24, Nr. 6, e12365, 08.2017.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Just, J., Lykkemark, S., Nielsen, C. H., Roshenas, A. R., Drasbek, K. R., Petersen, S. V., ... Kristensen, P. (2017). Pericyte modulation by a functional antibody obtained by a novel single cell selection strategy. Microcirculation, 24(6), [e12365]. https://doi.org/10.1111/micc.12365

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Just, Jesper ; Lykkemark, Simon ; Nielsen, Charlotte Høgsbjerg ; Roshenas, Ali Reza ; Drasbek, Kim Ryun ; Petersen, Steen Vang ; Bek, Toke ; Kristensen, Peter. / Pericyte modulation by a functional antibody obtained by a novel single cell selection strategy. I: Microcirculation. 2017 ; Bind 24, Nr. 6.

Bibtex

@article{44a52ce6cf3041c7ae7bf3070d781017,
title = "Pericyte modulation by a functional antibody obtained by a novel single cell selection strategy",
abstract = "OBJECTIVE: Pericytes surround the endothelial cells of the microvasculature where they serve as active participants in crucial vascular functions such as angiogenesis, stability, and permeability. However, pericyte loss or dysfunction has been described in a number of pathologies. Targeting pericytes could therefore prove instrumental in the further development of vascular therapeutics.METHODS: In order to target the pericyte, a proteomic-based approach using antibody phage display was conducted. We present a novel single cell selection strategy, with a modified selection step to drive the selection of antibodies towards relevant pericyte epitopes.RESULTS: Characterization of the selected antibodies revealed two antibodies with binding specificity for pericytes. The cognate antigen of one of the antibodies was identified as pericyte-expressed fibronectin. This antibody was shown to be a potent inhibitor of pericyte migration and to induce a pro-angiogenic response when included in a pericyte-endothelial cell co-culture angiogenesis assay.CONCLUSIONS: The selection method provides an efficient platform for the selection of functional antibodies which target pericytes. We obtain an antibody that interacts with a fibronectin epitope important for pericyte mobility and functionality. Targeting of this epitope in pathologies where pericytes are implicated could potentially be of therapeutic benefit. This article is protected by copyright. All rights reserved.",
keywords = "Journal Article",
author = "Jesper Just and Simon Lykkemark and Nielsen, {Charlotte H{\o}gsbjerg} and Roshenas, {Ali Reza} and Drasbek, {Kim Ryun} and Petersen, {Steen Vang} and Toke Bek and Peter Kristensen",
note = "This article is protected by copyright. All rights reserved.",
year = "2017",
month = "8",
doi = "10.1111/micc.12365",
language = "English",
volume = "24",
journal = "Microcirculation",
issn = "1073-9688",
publisher = "JohnWiley & Sons Ltd.",
number = "6",

}

RIS

TY - JOUR

T1 - Pericyte modulation by a functional antibody obtained by a novel single cell selection strategy

AU - Just, Jesper

AU - Lykkemark, Simon

AU - Nielsen, Charlotte Høgsbjerg

AU - Roshenas, Ali Reza

AU - Drasbek, Kim Ryun

AU - Petersen, Steen Vang

AU - Bek, Toke

AU - Kristensen, Peter

N1 - This article is protected by copyright. All rights reserved.

PY - 2017/8

Y1 - 2017/8

N2 - OBJECTIVE: Pericytes surround the endothelial cells of the microvasculature where they serve as active participants in crucial vascular functions such as angiogenesis, stability, and permeability. However, pericyte loss or dysfunction has been described in a number of pathologies. Targeting pericytes could therefore prove instrumental in the further development of vascular therapeutics.METHODS: In order to target the pericyte, a proteomic-based approach using antibody phage display was conducted. We present a novel single cell selection strategy, with a modified selection step to drive the selection of antibodies towards relevant pericyte epitopes.RESULTS: Characterization of the selected antibodies revealed two antibodies with binding specificity for pericytes. The cognate antigen of one of the antibodies was identified as pericyte-expressed fibronectin. This antibody was shown to be a potent inhibitor of pericyte migration and to induce a pro-angiogenic response when included in a pericyte-endothelial cell co-culture angiogenesis assay.CONCLUSIONS: The selection method provides an efficient platform for the selection of functional antibodies which target pericytes. We obtain an antibody that interacts with a fibronectin epitope important for pericyte mobility and functionality. Targeting of this epitope in pathologies where pericytes are implicated could potentially be of therapeutic benefit. This article is protected by copyright. All rights reserved.

AB - OBJECTIVE: Pericytes surround the endothelial cells of the microvasculature where they serve as active participants in crucial vascular functions such as angiogenesis, stability, and permeability. However, pericyte loss or dysfunction has been described in a number of pathologies. Targeting pericytes could therefore prove instrumental in the further development of vascular therapeutics.METHODS: In order to target the pericyte, a proteomic-based approach using antibody phage display was conducted. We present a novel single cell selection strategy, with a modified selection step to drive the selection of antibodies towards relevant pericyte epitopes.RESULTS: Characterization of the selected antibodies revealed two antibodies with binding specificity for pericytes. The cognate antigen of one of the antibodies was identified as pericyte-expressed fibronectin. This antibody was shown to be a potent inhibitor of pericyte migration and to induce a pro-angiogenic response when included in a pericyte-endothelial cell co-culture angiogenesis assay.CONCLUSIONS: The selection method provides an efficient platform for the selection of functional antibodies which target pericytes. We obtain an antibody that interacts with a fibronectin epitope important for pericyte mobility and functionality. Targeting of this epitope in pathologies where pericytes are implicated could potentially be of therapeutic benefit. This article is protected by copyright. All rights reserved.

KW - Journal Article

U2 - 10.1111/micc.12365

DO - 10.1111/micc.12365

M3 - Journal article

C2 - 28236639

VL - 24

JO - Microcirculation

JF - Microcirculation

SN - 1073-9688

IS - 6

M1 - e12365

ER -