Pathological and microbiological impact of a gentamicin-loaded biocomposite following limited or extensive debridement in a porcine model of osteomyelitis

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

Pathological and microbiological impact of a gentamicin-loaded biocomposite following limited or extensive debridement in a porcine model of osteomyelitis. / Blirup-Plum, Sophie A.; Bjarnsholt, T.; Jensen, H. E.; Kragh, K. N.; Aalbæk, B.; Gottlieb, H.; Bue, M.; Jensen, L. K.

I: Bone and Joint Research, Bind 9, Nr. 7, 07.2020, s. 394-401.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Blirup-Plum, SA, Bjarnsholt, T, Jensen, HE, Kragh, KN, Aalbæk, B, Gottlieb, H, Bue, M & Jensen, LK 2020, 'Pathological and microbiological impact of a gentamicin-loaded biocomposite following limited or extensive debridement in a porcine model of osteomyelitis', Bone and Joint Research, bind 9, nr. 7, s. 394-401. https://doi.org/10.1302/2046-3758.97.BJR-2020-0007.R1

APA

Blirup-Plum, S. A., Bjarnsholt, T., Jensen, H. E., Kragh, K. N., Aalbæk, B., Gottlieb, H., Bue, M., & Jensen, L. K. (2020). Pathological and microbiological impact of a gentamicin-loaded biocomposite following limited or extensive debridement in a porcine model of osteomyelitis. Bone and Joint Research, 9(7), 394-401. https://doi.org/10.1302/2046-3758.97.BJR-2020-0007.R1

CBE

MLA

Vancouver

Author

Blirup-Plum, Sophie A. ; Bjarnsholt, T. ; Jensen, H. E. ; Kragh, K. N. ; Aalbæk, B. ; Gottlieb, H. ; Bue, M. ; Jensen, L. K. / Pathological and microbiological impact of a gentamicin-loaded biocomposite following limited or extensive debridement in a porcine model of osteomyelitis. I: Bone and Joint Research. 2020 ; Bind 9, Nr. 7. s. 394-401.

Bibtex

@article{660040b2fa7445e6903e9fd0971c323f,
title = "Pathological and microbiological impact of a gentamicin-loaded biocomposite following limited or extensive debridement in a porcine model of osteomyelitis",
abstract = "Aims: CERAMENT|G is an absorbable gentamicin-loaded biocomposite used as an on-site vehicle of antimicrobials for the treatment of chronic osteomyelitis. The purpose of the present study was to investigate the sole effect of CERAMENT|G, i.e. without additional systemic antimicrobial therapy, in relation to a limited or extensive debridement of osteomyelitis lesions in a porcine model. Methods: Osteomyelitis was induced in nine pigs by inoculation of 104 colony-forming units (CFUs) of Staphylococcus aureus into a drill hole in the right tibia. After one week, the pigs were allocated into three groups. Group A (n = 3) received no treatment during the study period (19 days). Groups B (n = 3) and C (n = 3) received limited or extensive debridement seven days postinoculation, respectively, followed by injection of CERAMENT|G into the bone voids. The pigs were euthanized ten (Group C) and 12 (Group B) days after the intervention. Results: All animals presented confirmatory signs of bone infection post-mortem. The estimated amount of inflammation was substantially greater in Groups A and B compared to Group C. In both Groups B and C, peptide nucleic acid fluorescence in situ hybridization (PNA FISH) of CERAMENT|G and surrounding bone tissue revealed bacteria embedded in an opaque matrix, i.e. within biofilm. In addition, in Group C, the maximal measured post-mortem gentamicin concentrations in CERAMENT|G and surrounding bone tissue samples were 16.6 µg/ml and 6.2 µg/ml, respectively. Conclusion: The present study demonstrates that CERAMENT|G cannot be used as a standalone alternative to extensive debridement or be used without the addition of systemic antimicrobials.",
keywords = "Animal model, Antimicrobial carrier, Calcium sulphate, Hydroxyapatite, Osteomyelitis",
author = "Blirup-Plum, {Sophie A.} and T. Bjarnsholt and Jensen, {H. E.} and Kragh, {K. N.} and B. Aalb{\ae}k and H. Gottlieb and M. Bue and Jensen, {L. K.}",
year = "2020",
month = jul,
doi = "10.1302/2046-3758.97.BJR-2020-0007.R1",
language = "English",
volume = "9",
pages = "394--401",
journal = "Bone & Joint Research",
issn = "2046-3758",
publisher = "BRITISH EDITORIAL SOC BONE JOINT SURGERY",
number = "7",

}

RIS

TY - JOUR

T1 - Pathological and microbiological impact of a gentamicin-loaded biocomposite following limited or extensive debridement in a porcine model of osteomyelitis

AU - Blirup-Plum, Sophie A.

AU - Bjarnsholt, T.

AU - Jensen, H. E.

AU - Kragh, K. N.

AU - Aalbæk, B.

AU - Gottlieb, H.

AU - Bue, M.

AU - Jensen, L. K.

PY - 2020/7

Y1 - 2020/7

N2 - Aims: CERAMENT|G is an absorbable gentamicin-loaded biocomposite used as an on-site vehicle of antimicrobials for the treatment of chronic osteomyelitis. The purpose of the present study was to investigate the sole effect of CERAMENT|G, i.e. without additional systemic antimicrobial therapy, in relation to a limited or extensive debridement of osteomyelitis lesions in a porcine model. Methods: Osteomyelitis was induced in nine pigs by inoculation of 104 colony-forming units (CFUs) of Staphylococcus aureus into a drill hole in the right tibia. After one week, the pigs were allocated into three groups. Group A (n = 3) received no treatment during the study period (19 days). Groups B (n = 3) and C (n = 3) received limited or extensive debridement seven days postinoculation, respectively, followed by injection of CERAMENT|G into the bone voids. The pigs were euthanized ten (Group C) and 12 (Group B) days after the intervention. Results: All animals presented confirmatory signs of bone infection post-mortem. The estimated amount of inflammation was substantially greater in Groups A and B compared to Group C. In both Groups B and C, peptide nucleic acid fluorescence in situ hybridization (PNA FISH) of CERAMENT|G and surrounding bone tissue revealed bacteria embedded in an opaque matrix, i.e. within biofilm. In addition, in Group C, the maximal measured post-mortem gentamicin concentrations in CERAMENT|G and surrounding bone tissue samples were 16.6 µg/ml and 6.2 µg/ml, respectively. Conclusion: The present study demonstrates that CERAMENT|G cannot be used as a standalone alternative to extensive debridement or be used without the addition of systemic antimicrobials.

AB - Aims: CERAMENT|G is an absorbable gentamicin-loaded biocomposite used as an on-site vehicle of antimicrobials for the treatment of chronic osteomyelitis. The purpose of the present study was to investigate the sole effect of CERAMENT|G, i.e. without additional systemic antimicrobial therapy, in relation to a limited or extensive debridement of osteomyelitis lesions in a porcine model. Methods: Osteomyelitis was induced in nine pigs by inoculation of 104 colony-forming units (CFUs) of Staphylococcus aureus into a drill hole in the right tibia. After one week, the pigs were allocated into three groups. Group A (n = 3) received no treatment during the study period (19 days). Groups B (n = 3) and C (n = 3) received limited or extensive debridement seven days postinoculation, respectively, followed by injection of CERAMENT|G into the bone voids. The pigs were euthanized ten (Group C) and 12 (Group B) days after the intervention. Results: All animals presented confirmatory signs of bone infection post-mortem. The estimated amount of inflammation was substantially greater in Groups A and B compared to Group C. In both Groups B and C, peptide nucleic acid fluorescence in situ hybridization (PNA FISH) of CERAMENT|G and surrounding bone tissue revealed bacteria embedded in an opaque matrix, i.e. within biofilm. In addition, in Group C, the maximal measured post-mortem gentamicin concentrations in CERAMENT|G and surrounding bone tissue samples were 16.6 µg/ml and 6.2 µg/ml, respectively. Conclusion: The present study demonstrates that CERAMENT|G cannot be used as a standalone alternative to extensive debridement or be used without the addition of systemic antimicrobials.

KW - Animal model

KW - Antimicrobial carrier

KW - Calcium sulphate

KW - Hydroxyapatite

KW - Osteomyelitis

UR - http://www.scopus.com/inward/record.url?scp=85089994051&partnerID=8YFLogxK

U2 - 10.1302/2046-3758.97.BJR-2020-0007.R1

DO - 10.1302/2046-3758.97.BJR-2020-0007.R1

M3 - Journal article

C2 - 32793334

AN - SCOPUS:85089994051

VL - 9

SP - 394

EP - 401

JO - Bone & Joint Research

JF - Bone & Joint Research

SN - 2046-3758

IS - 7

ER -