Parasite worm antigens instruct macrophages to release immunoregulatory extracellular vesicles

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Parasite worm antigens instruct macrophages to release immunoregulatory extracellular vesicles. / Zakeri, Amin; Whitehead, Bradley J.; Stensballe, Allan et al.
I: Journal of Extracellular Vesicles, Bind 10, Nr. 10, e12131, 08.2021.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Zakeri A, Whitehead BJ, Stensballe A, de Korne C, Williams AR, Everts B et al. Parasite worm antigens instruct macrophages to release immunoregulatory extracellular vesicles. Journal of Extracellular Vesicles. 2021 aug.;10(10):e12131. doi: 10.1002/jev2.12131

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@article{3af9ac67ba6546c7aa44017c20d9112b,
title = "Parasite worm antigens instruct macrophages to release immunoregulatory extracellular vesicles",
abstract = "Emerging evidence suggests that immune cells not only communicate with each other through cytokines, chemokines, and cell surface receptors, but also by releasing small membranous structures known as extracellular vesicles (EVs). EVs carry a variety of different molecules that can be taken up by recipient cells. Parasitic worms are well known for their immunomodulatory properties, but whether they can affect immune responses by altering EV-driven communication between host immune cells remains unclear. Here we provide evidence that stimulation of bone marrow-derived macrophages (BMDMs) with soluble products of Trichuris suis (TSPs), leads to the release of EVs with anti-inflammatory properties. Specifically, we found that EVs from TSP-pulsed BMDMs, but not those from unstimulated BMDMs can suppress TNFα and IL-6 release in LPS-stimulated BMDMs and BMDCs. However, no polarization toward M1 or M2 was observed in macrophages exposed to EVs. Moreover, EVs enhanced reactive oxygen species (ROS) production in the exposed BMDMs, which was associated with a deregulated redox homeostasis as revealed by pathway analysis of transcriptomic data. Proteomic analysis identified cytochrome p450 (CYP450) as a potential source of ROS in EVs from TSP-pulsed BMDMs. Finally, pharmacological inhibition of CYP450 activity could suppress ROS production in those BMDMs. In summary, we find that TSPs can modulate immune responses not only via direct interactions but also indirectly by eliciting the release of EVs from BMDMs that exert anti-inflammatory effects on recipient cells.",
keywords = "extracellular vesicles, inflammation, macrophages, reactive oxygen species, Trichuris suis soluble products",
author = "Amin Zakeri and Whitehead, {Bradley J.} and Allan Stensballe and {de Korne}, Clarize and Williams, {Andrew R.} and Bart Everts and Peter Nejsum",
note = "Publisher Copyright: {\textcopyright} 2021 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles",
year = "2021",
month = aug,
doi = "10.1002/jev2.12131",
language = "English",
volume = "10",
journal = "Journal of Extracellular Vesicles",
issn = "2001-3078",
publisher = "Co-Action Publishing",
number = "10",

}

RIS

TY - JOUR

T1 - Parasite worm antigens instruct macrophages to release immunoregulatory extracellular vesicles

AU - Zakeri, Amin

AU - Whitehead, Bradley J.

AU - Stensballe, Allan

AU - de Korne, Clarize

AU - Williams, Andrew R.

AU - Everts, Bart

AU - Nejsum, Peter

N1 - Publisher Copyright: © 2021 The Authors. Journal of Extracellular Vesicles published by Wiley Periodicals, LLC on behalf of the International Society for Extracellular Vesicles

PY - 2021/8

Y1 - 2021/8

N2 - Emerging evidence suggests that immune cells not only communicate with each other through cytokines, chemokines, and cell surface receptors, but also by releasing small membranous structures known as extracellular vesicles (EVs). EVs carry a variety of different molecules that can be taken up by recipient cells. Parasitic worms are well known for their immunomodulatory properties, but whether they can affect immune responses by altering EV-driven communication between host immune cells remains unclear. Here we provide evidence that stimulation of bone marrow-derived macrophages (BMDMs) with soluble products of Trichuris suis (TSPs), leads to the release of EVs with anti-inflammatory properties. Specifically, we found that EVs from TSP-pulsed BMDMs, but not those from unstimulated BMDMs can suppress TNFα and IL-6 release in LPS-stimulated BMDMs and BMDCs. However, no polarization toward M1 or M2 was observed in macrophages exposed to EVs. Moreover, EVs enhanced reactive oxygen species (ROS) production in the exposed BMDMs, which was associated with a deregulated redox homeostasis as revealed by pathway analysis of transcriptomic data. Proteomic analysis identified cytochrome p450 (CYP450) as a potential source of ROS in EVs from TSP-pulsed BMDMs. Finally, pharmacological inhibition of CYP450 activity could suppress ROS production in those BMDMs. In summary, we find that TSPs can modulate immune responses not only via direct interactions but also indirectly by eliciting the release of EVs from BMDMs that exert anti-inflammatory effects on recipient cells.

AB - Emerging evidence suggests that immune cells not only communicate with each other through cytokines, chemokines, and cell surface receptors, but also by releasing small membranous structures known as extracellular vesicles (EVs). EVs carry a variety of different molecules that can be taken up by recipient cells. Parasitic worms are well known for their immunomodulatory properties, but whether they can affect immune responses by altering EV-driven communication between host immune cells remains unclear. Here we provide evidence that stimulation of bone marrow-derived macrophages (BMDMs) with soluble products of Trichuris suis (TSPs), leads to the release of EVs with anti-inflammatory properties. Specifically, we found that EVs from TSP-pulsed BMDMs, but not those from unstimulated BMDMs can suppress TNFα and IL-6 release in LPS-stimulated BMDMs and BMDCs. However, no polarization toward M1 or M2 was observed in macrophages exposed to EVs. Moreover, EVs enhanced reactive oxygen species (ROS) production in the exposed BMDMs, which was associated with a deregulated redox homeostasis as revealed by pathway analysis of transcriptomic data. Proteomic analysis identified cytochrome p450 (CYP450) as a potential source of ROS in EVs from TSP-pulsed BMDMs. Finally, pharmacological inhibition of CYP450 activity could suppress ROS production in those BMDMs. In summary, we find that TSPs can modulate immune responses not only via direct interactions but also indirectly by eliciting the release of EVs from BMDMs that exert anti-inflammatory effects on recipient cells.

KW - extracellular vesicles

KW - inflammation

KW - macrophages

KW - reactive oxygen species

KW - Trichuris suis soluble products

UR - http://www.scopus.com/inward/record.url?scp=85112793829&partnerID=8YFLogxK

U2 - 10.1002/jev2.12131

DO - 10.1002/jev2.12131

M3 - Journal article

C2 - 34429858

AN - SCOPUS:85112793829

VL - 10

JO - Journal of Extracellular Vesicles

JF - Journal of Extracellular Vesicles

SN - 2001-3078

IS - 10

M1 - e12131

ER -