Paraglaciecola hydrolytica sp nov., a bacterium with hydrolytic activity against multiple seaweed-derived polysaccharides

Pernille Kjersgaard Bech; Mikkel Schultz-Johansen; Mikkel Andreas Glaring; Tristan Barbeyron; Mirjam Czjzek; Peter Stougaard

Paraglaciecola hydrolytica sp nov., a bacterium with hydrolytic activity against multiple seaweed-derived polysaccharides

Pernille Kjersgaard Bech, Mikkel Schultz-Johansen, Mikkel Andreas Glaring, Tristan Barbeyron, Mirjam Czjzek, Peter Stougaard

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

Abstract

A novel bacterial strain, S66 ^T, was isolated from eelgrass collected on the coastline of Zealand, Denmark. Polyphasic analyses involving phenotypic, phylogenetic and genomic methods were used to characterize strain S66 ^T. The strain was Gram-reaction-negative, rod-shaped, aerobic, and displayed growth at 10–25 °C (optimum 20–25 °C) and at pH 7–9 (optimum pH 7.5). Furthermore, strain S66 ^T grew on seaweed polysaccharides agar, agarose, porphyran, k-carrageenan, alginate and laminarin as sole carbon sources. Major fatty acids were C _16:0, C _16:1ω7c and C _18:1ω7c. The respiratory quinone was determined to be Q-8, and major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content was determined to be 42.2 mol%. Phylogenetic analyses based on the 16S rRNA gene and GyrB sequence comparisons showed that the bacterium was affiliated with the genus Paraglaciecola within the family Alteromonadaceae of the class Gammaproteobacteria. The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66 ^T and other members of the genus Paraglaciecola were 94–95%and 84–85 %, respectively. Based on the genome sequence of S66 ^T, the average nucleotide identity (ANI) between strain S66 ^T and other members of the genus Paraglaciecola was 77–80 %, and DNA–DNA hybridization prediction showed values of less than 24% relatedness, respectively, between S66 ^T and other species of the genus Paraglaciecola. The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain S66 ^T represents a novel species of the genus Paraglaciecola, for which the name Paraglaciecola hydrolytica sp. nov. is proposed. The type strain is S66 ^T (=LMG 29457 ^T=NCIMB 15060 ^T=DSM 102834 ^T).

Originalsprog	Engelsk
Tidsskrift	International Journal of Systematic and Evolutionary Microbiology
Vol/bind	67
Nummer	7
Sider (fra-til)	2242-2247
Antal sider	6
ISSN	1466-5026
Status	Udgivet - 2017
Udgivet eksternt	Ja

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@article{4d41c1f3d0034794ac1a2882e504ef74,

title = "Paraglaciecola hydrolytica sp nov., a bacterium with hydrolytic activity against multiple seaweed-derived polysaccharides",

abstract = "A novel bacterial strain, S66 T, was isolated from eelgrass collected on the coastline of Zealand, Denmark. Polyphasic analyses involving phenotypic, phylogenetic and genomic methods were used to characterize strain S66 T. The strain was Gram-reaction-negative, rod-shaped, aerobic, and displayed growth at 10–25 °C (optimum 20–25 °C) and at pH 7–9 (optimum pH 7.5). Furthermore, strain S66 T grew on seaweed polysaccharides agar, agarose, porphyran, k-carrageenan, alginate and laminarin as sole carbon sources. Major fatty acids were C 16:0, C 16:1ω7c and C 18:1ω7c. The respiratory quinone was determined to be Q-8, and major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content was determined to be 42.2 mol%. Phylogenetic analyses based on the 16S rRNA gene and GyrB sequence comparisons showed that the bacterium was affiliated with the genus Paraglaciecola within the family Alteromonadaceae of the class Gammaproteobacteria. The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66 T and other members of the genus Paraglaciecola were 94–95%and 84–85 %, respectively. Based on the genome sequence of S66 T, the average nucleotide identity (ANI) between strain S66 T and other members of the genus Paraglaciecola was 77–80 %, and DNA–DNA hybridization prediction showed values of less than 24% relatedness, respectively, between S66 T and other species of the genus Paraglaciecola. The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain S66 T represents a novel species of the genus Paraglaciecola, for which the name Paraglaciecola hydrolytica sp. nov. is proposed. The type strain is S66 T (=LMG 29457 T=NCIMB 15060 T=DSM 102834 T).",

keywords = "Agarase, Carrageenase, Hydrolytic enzymes, Psychrotrophic",

author = "Bech, {Pernille Kjersgaard} and Mikkel Schultz-Johansen and Glaring, {Mikkel Andreas} and Tristan Barbeyron and Mirjam Czjzek and Peter Stougaard",

year = "2017",

language = "English",

volume = "67",

pages = "2242--2247",

journal = "International Journal of Systematic and Evolutionary Microbiology",

issn = "1466-5026",

publisher = "AMER SOC MICROBIOLOGY",

number = "7",

}

Paraglaciecola hydrolytica sp nov., a bacterium with hydrolytic activity against multiple seaweed-derived polysaccharides. / Bech, Pernille Kjersgaard; Schultz-Johansen, Mikkel; Glaring, Mikkel Andreas et al.
I: International Journal of Systematic and Evolutionary Microbiology, Bind 67, Nr. 7, 2017, s. 2242-2247.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

TY - JOUR

T1 - Paraglaciecola hydrolytica sp nov., a bacterium with hydrolytic activity against multiple seaweed-derived polysaccharides

AU - Bech, Pernille Kjersgaard

AU - Schultz-Johansen, Mikkel

AU - Glaring, Mikkel Andreas

AU - Barbeyron, Tristan

AU - Czjzek, Mirjam

AU - Stougaard, Peter

PY - 2017

Y1 - 2017

N2 - A novel bacterial strain, S66 T, was isolated from eelgrass collected on the coastline of Zealand, Denmark. Polyphasic analyses involving phenotypic, phylogenetic and genomic methods were used to characterize strain S66 T. The strain was Gram-reaction-negative, rod-shaped, aerobic, and displayed growth at 10–25 °C (optimum 20–25 °C) and at pH 7–9 (optimum pH 7.5). Furthermore, strain S66 T grew on seaweed polysaccharides agar, agarose, porphyran, k-carrageenan, alginate and laminarin as sole carbon sources. Major fatty acids were C 16:0, C 16:1ω7c and C 18:1ω7c. The respiratory quinone was determined to be Q-8, and major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content was determined to be 42.2 mol%. Phylogenetic analyses based on the 16S rRNA gene and GyrB sequence comparisons showed that the bacterium was affiliated with the genus Paraglaciecola within the family Alteromonadaceae of the class Gammaproteobacteria. The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66 T and other members of the genus Paraglaciecola were 94–95%and 84–85 %, respectively. Based on the genome sequence of S66 T, the average nucleotide identity (ANI) between strain S66 T and other members of the genus Paraglaciecola was 77–80 %, and DNA–DNA hybridization prediction showed values of less than 24% relatedness, respectively, between S66 T and other species of the genus Paraglaciecola. The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain S66 T represents a novel species of the genus Paraglaciecola, for which the name Paraglaciecola hydrolytica sp. nov. is proposed. The type strain is S66 T (=LMG 29457 T=NCIMB 15060 T=DSM 102834 T).

AB - A novel bacterial strain, S66 T, was isolated from eelgrass collected on the coastline of Zealand, Denmark. Polyphasic analyses involving phenotypic, phylogenetic and genomic methods were used to characterize strain S66 T. The strain was Gram-reaction-negative, rod-shaped, aerobic, and displayed growth at 10–25 °C (optimum 20–25 °C) and at pH 7–9 (optimum pH 7.5). Furthermore, strain S66 T grew on seaweed polysaccharides agar, agarose, porphyran, k-carrageenan, alginate and laminarin as sole carbon sources. Major fatty acids were C 16:0, C 16:1ω7c and C 18:1ω7c. The respiratory quinone was determined to be Q-8, and major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. The DNA G+C content was determined to be 42.2 mol%. Phylogenetic analyses based on the 16S rRNA gene and GyrB sequence comparisons showed that the bacterium was affiliated with the genus Paraglaciecola within the family Alteromonadaceae of the class Gammaproteobacteria. The percentage similarity between the 16S rRNA gene and GyrB sequences of strain S66 T and other members of the genus Paraglaciecola were 94–95%and 84–85 %, respectively. Based on the genome sequence of S66 T, the average nucleotide identity (ANI) between strain S66 T and other members of the genus Paraglaciecola was 77–80 %, and DNA–DNA hybridization prediction showed values of less than 24% relatedness, respectively, between S66 T and other species of the genus Paraglaciecola. The phenotypic, phylogenetic and genomic analyses support the hypothesis that strain S66 T represents a novel species of the genus Paraglaciecola, for which the name Paraglaciecola hydrolytica sp. nov. is proposed. The type strain is S66 T (=LMG 29457 T=NCIMB 15060 T=DSM 102834 T).

KW - Agarase

KW - Carrageenase

KW - Hydrolytic enzymes

KW - Psychrotrophic

UR - http://www.scopus.com/inward/record.url?scp=85027121143&partnerID=8YFLogxK

M3 - Journal article

SN - 1466-5026

VL - 67

SP - 2242

EP - 2247

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

IS - 7

ER -

Paraglaciecola hydrolytica sp nov., a bacterium with hydrolytic activity against multiple seaweed-derived polysaccharides

Abstract

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