p38MAPK/MK2-mediated phosphorylation of RBM7 regulates the human nuclear exosome targeting complex

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p38MAPK/MK2-mediated phosphorylation of RBM7 regulates the human nuclear exosome targeting complex. / Tiedje, Christopher; Lubas, Michal; Tehrani, Mohammad; Menon, Manoj B.; Ronkina, Natalia; Rousseau, Simon; Cohen, Philip; Kotlyarov, Alexey; Gaestel, Matthias.

I: RNA, Bind 21, Nr. 2, 2015, s. 262-278.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Tiedje, C, Lubas, M, Tehrani, M, Menon, MB, Ronkina, N, Rousseau, S, Cohen, P, Kotlyarov, A & Gaestel, M 2015, 'p38MAPK/MK2-mediated phosphorylation of RBM7 regulates the human nuclear exosome targeting complex', RNA, bind 21, nr. 2, s. 262-278. https://doi.org/10.1261/rna.048090.114

APA

Tiedje, C., Lubas, M., Tehrani, M., Menon, M. B., Ronkina, N., Rousseau, S., Cohen, P., Kotlyarov, A., & Gaestel, M. (2015). p38MAPK/MK2-mediated phosphorylation of RBM7 regulates the human nuclear exosome targeting complex. RNA, 21(2), 262-278. https://doi.org/10.1261/rna.048090.114

CBE

Tiedje C, Lubas M, Tehrani M, Menon MB, Ronkina N, Rousseau S, Cohen P, Kotlyarov A, Gaestel M. 2015. p38MAPK/MK2-mediated phosphorylation of RBM7 regulates the human nuclear exosome targeting complex. RNA. 21(2):262-278. https://doi.org/10.1261/rna.048090.114

MLA

Vancouver

Tiedje C, Lubas M, Tehrani M, Menon MB, Ronkina N, Rousseau S o.a. p38MAPK/MK2-mediated phosphorylation of RBM7 regulates the human nuclear exosome targeting complex. RNA. 2015;21(2):262-278. https://doi.org/10.1261/rna.048090.114

Author

Tiedje, Christopher ; Lubas, Michal ; Tehrani, Mohammad ; Menon, Manoj B. ; Ronkina, Natalia ; Rousseau, Simon ; Cohen, Philip ; Kotlyarov, Alexey ; Gaestel, Matthias. / p38MAPK/MK2-mediated phosphorylation of RBM7 regulates the human nuclear exosome targeting complex. I: RNA. 2015 ; Bind 21, Nr. 2. s. 262-278.

Bibtex

@article{f1f5c5f1546548d998b42403f969080e,
title = "p38MAPK/MK2-mediated phosphorylation of RBM7 regulates the human nuclear exosome targeting complex",
abstract = "The nuclear exosome targeting complex (NEXT) directs a major 3'-5' exonuclease, the RNA exosome, for degradation of nuclear noncoding (nc) RNAs. We identified the RNA-binding component of the NEXT complex, RBM7, as a substrate of p38MAPK/MK2-mediated phosphorylation at residue S136. As a result of this phosphorylation, RBM7 displays a strongly decreased RNA-binding capacity, while inhibition of p38MAPK or mutation of S136A in RBM7 increases its RNA association. Interestingly, promoter-upstream transcripts (PROMPTs), such as proRBM39, proEXT1, proDNAJB4, accumulated upon stress stimulation in a p38MAPK/MK2-dependent manner, a process inhibited by overexpression of RBM7S136A. While there are no stress-dependent changes in RNA-polymerase II (RNAPII) occupation of PROMPT regions representing unchanged transcription, stability of PROMPTs is increased. Hence, we propose that phosphorylation of RBM7 by the p38MAPK/MK2 axis increases nuclear ncRNA stability by blocking their RBM7-binding and subsequent RNA exosome targeting to allow stress-dependent modulations of the noncoding transcriptome.",
keywords = "ncRNAs, p38/MK2 signaling, Phosphorylation, RBM7, RNA binding, RNA stability",
author = "Christopher Tiedje and Michal Lubas and Mohammad Tehrani and Menon, {Manoj B.} and Natalia Ronkina and Simon Rousseau and Philip Cohen and Alexey Kotlyarov and Matthias Gaestel",
year = "2015",
doi = "10.1261/rna.048090.114",
language = "English",
volume = "21",
pages = "262--278",
journal = "RNA",
issn = "1355-8382",
publisher = "Cold Spring Harbor Laboratory Press",
number = "2",

}

RIS

TY - JOUR

T1 - p38MAPK/MK2-mediated phosphorylation of RBM7 regulates the human nuclear exosome targeting complex

AU - Tiedje, Christopher

AU - Lubas, Michal

AU - Tehrani, Mohammad

AU - Menon, Manoj B.

AU - Ronkina, Natalia

AU - Rousseau, Simon

AU - Cohen, Philip

AU - Kotlyarov, Alexey

AU - Gaestel, Matthias

PY - 2015

Y1 - 2015

N2 - The nuclear exosome targeting complex (NEXT) directs a major 3'-5' exonuclease, the RNA exosome, for degradation of nuclear noncoding (nc) RNAs. We identified the RNA-binding component of the NEXT complex, RBM7, as a substrate of p38MAPK/MK2-mediated phosphorylation at residue S136. As a result of this phosphorylation, RBM7 displays a strongly decreased RNA-binding capacity, while inhibition of p38MAPK or mutation of S136A in RBM7 increases its RNA association. Interestingly, promoter-upstream transcripts (PROMPTs), such as proRBM39, proEXT1, proDNAJB4, accumulated upon stress stimulation in a p38MAPK/MK2-dependent manner, a process inhibited by overexpression of RBM7S136A. While there are no stress-dependent changes in RNA-polymerase II (RNAPII) occupation of PROMPT regions representing unchanged transcription, stability of PROMPTs is increased. Hence, we propose that phosphorylation of RBM7 by the p38MAPK/MK2 axis increases nuclear ncRNA stability by blocking their RBM7-binding and subsequent RNA exosome targeting to allow stress-dependent modulations of the noncoding transcriptome.

AB - The nuclear exosome targeting complex (NEXT) directs a major 3'-5' exonuclease, the RNA exosome, for degradation of nuclear noncoding (nc) RNAs. We identified the RNA-binding component of the NEXT complex, RBM7, as a substrate of p38MAPK/MK2-mediated phosphorylation at residue S136. As a result of this phosphorylation, RBM7 displays a strongly decreased RNA-binding capacity, while inhibition of p38MAPK or mutation of S136A in RBM7 increases its RNA association. Interestingly, promoter-upstream transcripts (PROMPTs), such as proRBM39, proEXT1, proDNAJB4, accumulated upon stress stimulation in a p38MAPK/MK2-dependent manner, a process inhibited by overexpression of RBM7S136A. While there are no stress-dependent changes in RNA-polymerase II (RNAPII) occupation of PROMPT regions representing unchanged transcription, stability of PROMPTs is increased. Hence, we propose that phosphorylation of RBM7 by the p38MAPK/MK2 axis increases nuclear ncRNA stability by blocking their RBM7-binding and subsequent RNA exosome targeting to allow stress-dependent modulations of the noncoding transcriptome.

KW - ncRNAs

KW - p38/MK2 signaling

KW - Phosphorylation

KW - RBM7

KW - RNA binding

KW - RNA stability

UR - http://www.scopus.com/inward/record.url?scp=84921403504&partnerID=8YFLogxK

U2 - 10.1261/rna.048090.114

DO - 10.1261/rna.048090.114

M3 - Journal article

C2 - 25525152

AN - SCOPUS:84921403504

VL - 21

SP - 262

EP - 278

JO - RNA

JF - RNA

SN - 1355-8382

IS - 2

ER -