TY - JOUR
T1 - Osteoclast-like multinucleated giant cells reinforce polycaprolactone grafts
AU - Einarsson, Halldór Bjarki
AU - Mortensen, Anders Frisk
AU - Nielsen, Morten Schallburg
AU - Chen, Menglin
AU - Nielsen, Søren Roesgaard
AU - Kraft, David Christian Evar
AU - Jensen, Jonas
AU - Bjerre, Mette
AU - Nygaard, Jens Vinge
AU - Bünger, Cody Eric
AU - Vorup-Jensen, Thomas
PY - 2025
Y1 - 2025
N2 - Successful application of advanced engineered materials in osteoplasty requires a biological understanding of the recipient reaction. The immune system acts like a double-edged sword by maintaining targeted tissue and rejecting grafts. Nevertheless, even for promising graft materials such as polycaprolactone, insights on contact with immune cells have been restricted due to lacking quantitative assays. Here, we show that polycaprolactone graft sites after cranioplasty are dominated by an immature type of multinucleated giant cells, probably derived from transmigrating peripheral monocytes. The cells interact with the polycaprolactone through extensive pseudopodia formation and localized polymer dissolution. Dynamic mechanical analysis revealed osteoclast-like cells, derived in vitro from primary human monocytes, reinforce polycaprolactone by depositing a CD18 integrin-rich attachment matrix. Our findings give a new perspective on immune cells' beneficial and detrimental functions in graft lesions, guiding therapy with better graft designs.
AB - Successful application of advanced engineered materials in osteoplasty requires a biological understanding of the recipient reaction. The immune system acts like a double-edged sword by maintaining targeted tissue and rejecting grafts. Nevertheless, even for promising graft materials such as polycaprolactone, insights on contact with immune cells have been restricted due to lacking quantitative assays. Here, we show that polycaprolactone graft sites after cranioplasty are dominated by an immature type of multinucleated giant cells, probably derived from transmigrating peripheral monocytes. The cells interact with the polycaprolactone through extensive pseudopodia formation and localized polymer dissolution. Dynamic mechanical analysis revealed osteoclast-like cells, derived in vitro from primary human monocytes, reinforce polycaprolactone by depositing a CD18 integrin-rich attachment matrix. Our findings give a new perspective on immune cells' beneficial and detrimental functions in graft lesions, guiding therapy with better graft designs.
KW - Humans
KW - Polyesters
KW - Giant Cells/immunology
KW - Osteoclasts/immunology
KW - Cells, Cultured
KW - Monocytes
KW - Male
U2 - 10.3389/fimmu.2025.1572238
DO - 10.3389/fimmu.2025.1572238
M3 - Journal article
C2 - 40469291
SN - 1664-3224
VL - 16
JO - Frontiers in Immunology
JF - Frontiers in Immunology
M1 - 1572238
ER -