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Optogenetic control of gene expression in plants in the presence of ambient white light

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Optogenetic control of gene expression in plants in the presence of ambient white light. / Ochoa-Fernandez, Rocio; Abel, Nikolaj B.; Wieland, Franz Georg; Schlegel, Jenia; Koch, Leonie Alexa; Miller, J. Benjamin; Engesser, Raphael; Giuriani, Giovanni; Brandl, Simon M.; Timmer, Jens; Weber, Wilfried; Ott, Thomas; Simon, Rüdiger; Zurbriggen, Matias D.

I: Nature Methods, Bind 17, Nr. 7, 01.07.2020, s. 717-725.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Ochoa-Fernandez, R, Abel, NB, Wieland, FG, Schlegel, J, Koch, LA, Miller, JB, Engesser, R, Giuriani, G, Brandl, SM, Timmer, J, Weber, W, Ott, T, Simon, R & Zurbriggen, MD 2020, 'Optogenetic control of gene expression in plants in the presence of ambient white light', Nature Methods, bind 17, nr. 7, s. 717-725. https://doi.org/10.1038/s41592-020-0868-y

APA

Ochoa-Fernandez, R., Abel, N. B., Wieland, F. G., Schlegel, J., Koch, L. A., Miller, J. B., Engesser, R., Giuriani, G., Brandl, S. M., Timmer, J., Weber, W., Ott, T., Simon, R., & Zurbriggen, M. D. (2020). Optogenetic control of gene expression in plants in the presence of ambient white light. Nature Methods, 17(7), 717-725. https://doi.org/10.1038/s41592-020-0868-y

CBE

Ochoa-Fernandez R, Abel NB, Wieland FG, Schlegel J, Koch LA, Miller JB, Engesser R, Giuriani G, Brandl SM, Timmer J, Weber W, Ott T, Simon R, Zurbriggen MD. 2020. Optogenetic control of gene expression in plants in the presence of ambient white light. Nature Methods. 17(7):717-725. https://doi.org/10.1038/s41592-020-0868-y

MLA

Vancouver

Ochoa-Fernandez R, Abel NB, Wieland FG, Schlegel J, Koch LA, Miller JB o.a. Optogenetic control of gene expression in plants in the presence of ambient white light. Nature Methods. 2020 jul 1;17(7):717-725. https://doi.org/10.1038/s41592-020-0868-y

Author

Ochoa-Fernandez, Rocio ; Abel, Nikolaj B. ; Wieland, Franz Georg ; Schlegel, Jenia ; Koch, Leonie Alexa ; Miller, J. Benjamin ; Engesser, Raphael ; Giuriani, Giovanni ; Brandl, Simon M. ; Timmer, Jens ; Weber, Wilfried ; Ott, Thomas ; Simon, Rüdiger ; Zurbriggen, Matias D. / Optogenetic control of gene expression in plants in the presence of ambient white light. I: Nature Methods. 2020 ; Bind 17, Nr. 7. s. 717-725.

Bibtex

@article{c3552cb910694e09b848a9f2a848e500,
title = "Optogenetic control of gene expression in plants in the presence of ambient white light",
abstract = "Optogenetics is the genetic approach for controlling cellular processes with light. It provides spatiotemporal, quantitative and reversible control over biological signaling and metabolic processes, overcoming limitations of chemically inducible systems. However, optogenetics lags in plant research because ambient light required for growth leads to undesired system activation. We solved this issue by developing plant usable light-switch elements (PULSE), an optogenetic tool for reversibly controlling gene expression in plants under ambient light. PULSE combines a blue-light-regulated repressor with a red-light-inducible switch. Gene expression is only activated under red light and remains inactive under white light or in darkness. Supported by a quantitative mathematical model, we characterized PULSE in protoplasts and achieved high induction rates, and we combined it with CRISPR–Cas9-based technologies to target synthetic signaling and developmental pathways. We applied PULSE to control immune responses in plant leaves and generated Arabidopsis transgenic plants. PULSE opens broad experimental avenues in plant research and biotechnology.",
author = "Rocio Ochoa-Fernandez and Abel, {Nikolaj B.} and Wieland, {Franz Georg} and Jenia Schlegel and Koch, {Leonie Alexa} and Miller, {J. Benjamin} and Raphael Engesser and Giovanni Giuriani and Brandl, {Simon M.} and Jens Timmer and Wilfried Weber and Thomas Ott and R{\"u}diger Simon and Zurbriggen, {Matias D.}",
note = "Funding Information: This study was supported in part by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany{\textquoteright}s Excellence Strategy (CEPLAS—EXC-1028 project no. 194465578 to R.S. and M.D.Z., EXC-2048/1—project no. 390686111 to R.S. and M.D.Z., CIBSS – EXC-2189—project no. 390939984 to T.O., J.T. and W.W., and BIOSS – EXC-294 to J.T. and W.W.), the iGRAD Plant (IRTG 1525 to R.O.F., J.S., R.S. and M.D.Z.), and the Collaborative Research Centers SFB1208 (project no. 267205415; project A13 to M.D.Z.) and SFB924 (INST 95/1126-2; project B4 to T.O.), the European Commission – Research Executive Agency (H2020 Future and Emerging Technologies FET-Open project no. 801041 CyGenTig to M.D.Z.). J.B.M. is supported by a fellowship from the Eastern Academic Research Consortium. We thank D. Orzaez (Polytechnic University of Valencia) and K. Gardner (City University of New York) for kindly providing the GoldenBraid and EL222 plasmids, respectively, T. Brumbarova (University of D{\"u}sseldorf) for aid with quantitative reverse-transcription PCR experiments, R. Wurm and M. Gerads (University of D{\"u}sseldorf) for technical assistance, and J. Schmidt (Technical Workshop Biology, University of Freiburg) for designing and constructing the light boxes used in this work. We are indebted to J. Casal (University of Buenos Aires), D. Nusinow (Danforth Center), S. Romero, H. Beyer and U. Urquiza (University of D{\"u}sseldorf) for careful reading and their suggestions to improve the manuscript. Publisher Copyright: {\textcopyright} 2020, The Author(s), under exclusive licence to Springer Nature America, Inc. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2020",
month = jul,
day = "1",
doi = "10.1038/s41592-020-0868-y",
language = "English",
volume = "17",
pages = "717--725",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Publishing Group",
number = "7",

}

RIS

TY - JOUR

T1 - Optogenetic control of gene expression in plants in the presence of ambient white light

AU - Ochoa-Fernandez, Rocio

AU - Abel, Nikolaj B.

AU - Wieland, Franz Georg

AU - Schlegel, Jenia

AU - Koch, Leonie Alexa

AU - Miller, J. Benjamin

AU - Engesser, Raphael

AU - Giuriani, Giovanni

AU - Brandl, Simon M.

AU - Timmer, Jens

AU - Weber, Wilfried

AU - Ott, Thomas

AU - Simon, Rüdiger

AU - Zurbriggen, Matias D.

N1 - Funding Information: This study was supported in part by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) under Germany’s Excellence Strategy (CEPLAS—EXC-1028 project no. 194465578 to R.S. and M.D.Z., EXC-2048/1—project no. 390686111 to R.S. and M.D.Z., CIBSS – EXC-2189—project no. 390939984 to T.O., J.T. and W.W., and BIOSS – EXC-294 to J.T. and W.W.), the iGRAD Plant (IRTG 1525 to R.O.F., J.S., R.S. and M.D.Z.), and the Collaborative Research Centers SFB1208 (project no. 267205415; project A13 to M.D.Z.) and SFB924 (INST 95/1126-2; project B4 to T.O.), the European Commission – Research Executive Agency (H2020 Future and Emerging Technologies FET-Open project no. 801041 CyGenTig to M.D.Z.). J.B.M. is supported by a fellowship from the Eastern Academic Research Consortium. We thank D. Orzaez (Polytechnic University of Valencia) and K. Gardner (City University of New York) for kindly providing the GoldenBraid and EL222 plasmids, respectively, T. Brumbarova (University of Düsseldorf) for aid with quantitative reverse-transcription PCR experiments, R. Wurm and M. Gerads (University of Düsseldorf) for technical assistance, and J. Schmidt (Technical Workshop Biology, University of Freiburg) for designing and constructing the light boxes used in this work. We are indebted to J. Casal (University of Buenos Aires), D. Nusinow (Danforth Center), S. Romero, H. Beyer and U. Urquiza (University of Düsseldorf) for careful reading and their suggestions to improve the manuscript. Publisher Copyright: © 2020, The Author(s), under exclusive licence to Springer Nature America, Inc. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/7/1

Y1 - 2020/7/1

N2 - Optogenetics is the genetic approach for controlling cellular processes with light. It provides spatiotemporal, quantitative and reversible control over biological signaling and metabolic processes, overcoming limitations of chemically inducible systems. However, optogenetics lags in plant research because ambient light required for growth leads to undesired system activation. We solved this issue by developing plant usable light-switch elements (PULSE), an optogenetic tool for reversibly controlling gene expression in plants under ambient light. PULSE combines a blue-light-regulated repressor with a red-light-inducible switch. Gene expression is only activated under red light and remains inactive under white light or in darkness. Supported by a quantitative mathematical model, we characterized PULSE in protoplasts and achieved high induction rates, and we combined it with CRISPR–Cas9-based technologies to target synthetic signaling and developmental pathways. We applied PULSE to control immune responses in plant leaves and generated Arabidopsis transgenic plants. PULSE opens broad experimental avenues in plant research and biotechnology.

AB - Optogenetics is the genetic approach for controlling cellular processes with light. It provides spatiotemporal, quantitative and reversible control over biological signaling and metabolic processes, overcoming limitations of chemically inducible systems. However, optogenetics lags in plant research because ambient light required for growth leads to undesired system activation. We solved this issue by developing plant usable light-switch elements (PULSE), an optogenetic tool for reversibly controlling gene expression in plants under ambient light. PULSE combines a blue-light-regulated repressor with a red-light-inducible switch. Gene expression is only activated under red light and remains inactive under white light or in darkness. Supported by a quantitative mathematical model, we characterized PULSE in protoplasts and achieved high induction rates, and we combined it with CRISPR–Cas9-based technologies to target synthetic signaling and developmental pathways. We applied PULSE to control immune responses in plant leaves and generated Arabidopsis transgenic plants. PULSE opens broad experimental avenues in plant research and biotechnology.

UR - http://www.scopus.com/inward/record.url?scp=85087029624&partnerID=8YFLogxK

U2 - 10.1038/s41592-020-0868-y

DO - 10.1038/s41592-020-0868-y

M3 - Journal article

C2 - 32601426

AN - SCOPUS:85087029624

VL - 17

SP - 717

EP - 725

JO - Nature Methods

JF - Nature Methods

SN - 1548-7091

IS - 7

ER -