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Opportunities and Challenges of In Vitro Tissue Culture Systems in the Era of Crop Genome Editing

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisReviewForskningpeer review


Currently, the development of genome editing (GE) tools has provided a wide platform for targeted modification of plant genomes. However, the lack of versatile DNA delivery systems for a large variety of crop species has been the main bottleneck for improving crops with beneficial traits. Currently, the generation of plants with heritable mutations induced by GE tools mostly goes through tissue culture. Unfortunately, current tissue culture systems restrict successful results to only a limited number of plant species and genotypes. In order to release the full potential of the GE tools, procedures need to be species and genotype independent. This review provides an in-depth summary and insights into the various in vitro tissue culture systems used for GE in the economically important crops barley, wheat, rice, sorghum, soybean, maize, potatoes, cassava, and millet and uncovers new opportunities and challenges of already-established tissue culture platforms for GE in the crops.

TidsskriftInternational Journal of Molecular Sciences
StatusUdgivet - aug. 2023

Bibliografisk note

Funding Information:
The current study was supported by the ReTraQue project, Innovation Fund Denmark 8055-00038B.

Publisher Copyright:
© 2023 by the authors.

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