One-step purification of E. coli elongation factor Tu.

Charlotte Rohde Knudsen, Brian F. C. Clark, B Degn, Ove Wiborg

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    Abstract

    The tuf A gene, encoding the E. coli elongation factor Tu, was cloned in the pGEX gene fusion system. Upon expression EF-Tu is fused to glutathione-S-transferase serving as a purification handle with affinity for glutathione immobilised on agarose. This allows purification of EF-Tu in a one-step procedure. The construct was designed in order to make possible the release of authentic EF-Tu by cleaving the fusion protein with the protease factor Xa.
    Udgivelsesdato: 1992-Oct
    OriginalsprogEngelsk
    TidsskriftBiochemistry international
    Vol/bind28
    Nummer2
    Sider (fra-til)353-62
    Antal sider9
    ISSN0158-5231
    StatusUdgivet - 1993

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