Nutrients, surfactants, and aeration in constructed wetlands affect bacterial persistence and metabolic activity during the remediation of crude oil-contaminated water

Amer Jamal Hashmat; Muhammad Afzal; Samina Iqbal; Imran Amin; Carlos Alberto Arias; Hans Brix; Imran Zafar; Sania Riaz; Rizwan ur Rehman; Ahmad Mohammad Salamatullah; Gezahign Fentahun Wondmie; Mohammed Bourhia

doi:10.1186/s40643-024-00757-5

Nutrients, surfactants, and aeration in constructed wetlands affect bacterial persistence and metabolic activity during the remediation of crude oil-contaminated water

Amer Jamal Hashmat^*, Muhammad Afzal, Samina Iqbal, Imran Amin, Carlos Alberto Arias, Hans Brix, Imran Zafar, Sania Riaz, Rizwan ur Rehman, Ahmad Mohammad Salamatullah, Gezahign Fentahun Wondmie^*, Mohammed Bourhia

^*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

Abstract

The use of constructed wetlands (CWs) is one of the best options to treat wastewater. In CWs, microorganisms play a major role in the degradation of organic pollutants but the concentration of nutrients, surfactant, and aeration (NSA) in oil-contaminated water is one of the factors that affect the persistence and metabolic functioning of hydrocarbon-degrading microorganisms. In the present investigation, the influence of the addition of NSA on the persistence of the augmented bacteria, copy of (alkane hydroxylase gene) alkB gene, and its expression level in the water, soil, and plants of CWs were evaluated. The CWs mesocosms were developed by the vegetation of Typha latifolia and Cyperus laevigatus and inoculated with the bacterial consortium (Pseudomonas putida TYRI39, Acinetobacter junii TYRH47, Acinetobacter sp. CYRH17, Pseudomonas sp. CYSI27, and Pseudomonas sp. TYRH42). The mesocosms were provided with nutrients (20 mg l^{− 1} N, 2.6 mg l^{− 1} P, and 16.4 mg l^{− 1} K) in liquid form, surfactant Tween-20 (0.2%, v/v) in liquid form, and aeration (≥ 7.0 ± 1 mg l^{− 1} DO) using aeration pump. The addition of NSA in CWs enhanced the persistence and metabolic functioning of the inoculated bacteria in the water, rhizospheric soil, and plants. The maximum hydrocarbon removal (97%) was observed in the water treated by CWs having C. laevigatus, bacteria, and NSA, and it is correlated with the copy numbers of alkB and its expression level. The application of NSA in CWs not only improved bacterial persistence and catabolic gene expression but also increased plant development and hydrocarbon removal.

Originalsprog	Engelsk
Artikelnummer	40
Tidsskrift	Bioresources and Bioprocessing
Vol/bind	11
Nummer	1
Antal sider	8
DOI	https://doi.org/10.1186/s40643-024-00757-5
Status	Udgivet - dec. 2024

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10.1186/s40643-024-00757-5Licens: CC BY

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Hashmat, A. J., Afzal, M., Iqbal, S., Amin, I., Arias, C. A., Brix, H., Zafar, I., Riaz, S., Rehman, R. U., Salamatullah, A. M., Wondmie, G. F., & Bourhia, M. (2024). Nutrients, surfactants, and aeration in constructed wetlands affect bacterial persistence and metabolic activity during the remediation of crude oil-contaminated water. Bioresources and Bioprocessing, 11(1), Artikel 40. https://doi.org/10.1186/s40643-024-00757-5

@article{65f62180bb4040e390ea058339e96fc9,

title = "Nutrients, surfactants, and aeration in constructed wetlands affect bacterial persistence and metabolic activity during the remediation of crude oil-contaminated water",

abstract = "The use of constructed wetlands (CWs) is one of the best options to treat wastewater. In CWs, microorganisms play a major role in the degradation of organic pollutants but the concentration of nutrients, surfactant, and aeration (NSA) in oil-contaminated water is one of the factors that affect the persistence and metabolic functioning of hydrocarbon-degrading microorganisms. In the present investigation, the influence of the addition of NSA on the persistence of the augmented bacteria, copy of (alkane hydroxylase gene) alkB gene, and its expression level in the water, soil, and plants of CWs were evaluated. The CWs mesocosms were developed by the vegetation of Typha latifolia and Cyperus laevigatus and inoculated with the bacterial consortium (Pseudomonas putida TYRI39, Acinetobacter junii TYRH47, Acinetobacter sp. CYRH17, Pseudomonas sp. CYSI27, and Pseudomonas sp. TYRH42). The mesocosms were provided with nutrients (20 mg l− 1 N, 2.6 mg l− 1 P, and 16.4 mg l− 1 K) in liquid form, surfactant Tween-20 (0.2%, v/v) in liquid form, and aeration (≥ 7.0 ± 1 mg l− 1 DO) using aeration pump. The addition of NSA in CWs enhanced the persistence and metabolic functioning of the inoculated bacteria in the water, rhizospheric soil, and plants. The maximum hydrocarbon removal (97%) was observed in the water treated by CWs having C. laevigatus, bacteria, and NSA, and it is correlated with the copy numbers of alkB and its expression level. The application of NSA in CWs not only improved bacterial persistence and catabolic gene expression but also increased plant development and hydrocarbon removal.",

keywords = "Bacterial persistence, Constructed wetlands, Crude oil, Gene abundance and expression, Metabolic activity",

author = "Hashmat, {Amer Jamal} and Muhammad Afzal and Samina Iqbal and Imran Amin and Arias, {Carlos Alberto} and Hans Brix and Imran Zafar and Sania Riaz and Rehman, {Rizwan ur} and Salamatullah, {Ahmad Mohammad} and Wondmie, {Gezahign Fentahun} and Mohammed Bourhia",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2024.",

year = "2024",

month = dec,

doi = "10.1186/s40643-024-00757-5",

language = "English",

volume = "11",

journal = "Bioresources and Bioprocessing",

issn = "2197-4365",

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Hashmat, AJ, Afzal, M, Iqbal, S, Amin, I, Arias, CA , Brix, H, Zafar, I, Riaz, S, Rehman, RU, Salamatullah, AM, Wondmie, GF & Bourhia, M 2024, 'Nutrients, surfactants, and aeration in constructed wetlands affect bacterial persistence and metabolic activity during the remediation of crude oil-contaminated water', Bioresources and Bioprocessing, bind 11, nr. 1, 40. https://doi.org/10.1186/s40643-024-00757-5

Nutrients, surfactants, and aeration in constructed wetlands affect bacterial persistence and metabolic activity during the remediation of crude oil-contaminated water. / Hashmat, Amer Jamal; Afzal, Muhammad; Iqbal, Samina et al.
I: Bioresources and Bioprocessing, Bind 11, Nr. 1, 40, 12.2024.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review

TY - JOUR

T1 - Nutrients, surfactants, and aeration in constructed wetlands affect bacterial persistence and metabolic activity during the remediation of crude oil-contaminated water

AU - Hashmat, Amer Jamal

AU - Afzal, Muhammad

AU - Iqbal, Samina

AU - Amin, Imran

AU - Arias, Carlos Alberto

AU - Brix, Hans

AU - Zafar, Imran

AU - Riaz, Sania

AU - Rehman, Rizwan ur

AU - Salamatullah, Ahmad Mohammad

AU - Wondmie, Gezahign Fentahun

AU - Bourhia, Mohammed

PY - 2024/12

Y1 - 2024/12

N2 - The use of constructed wetlands (CWs) is one of the best options to treat wastewater. In CWs, microorganisms play a major role in the degradation of organic pollutants but the concentration of nutrients, surfactant, and aeration (NSA) in oil-contaminated water is one of the factors that affect the persistence and metabolic functioning of hydrocarbon-degrading microorganisms. In the present investigation, the influence of the addition of NSA on the persistence of the augmented bacteria, copy of (alkane hydroxylase gene) alkB gene, and its expression level in the water, soil, and plants of CWs were evaluated. The CWs mesocosms were developed by the vegetation of Typha latifolia and Cyperus laevigatus and inoculated with the bacterial consortium (Pseudomonas putida TYRI39, Acinetobacter junii TYRH47, Acinetobacter sp. CYRH17, Pseudomonas sp. CYSI27, and Pseudomonas sp. TYRH42). The mesocosms were provided with nutrients (20 mg l− 1 N, 2.6 mg l− 1 P, and 16.4 mg l− 1 K) in liquid form, surfactant Tween-20 (0.2%, v/v) in liquid form, and aeration (≥ 7.0 ± 1 mg l− 1 DO) using aeration pump. The addition of NSA in CWs enhanced the persistence and metabolic functioning of the inoculated bacteria in the water, rhizospheric soil, and plants. The maximum hydrocarbon removal (97%) was observed in the water treated by CWs having C. laevigatus, bacteria, and NSA, and it is correlated with the copy numbers of alkB and its expression level. The application of NSA in CWs not only improved bacterial persistence and catabolic gene expression but also increased plant development and hydrocarbon removal.

AB - The use of constructed wetlands (CWs) is one of the best options to treat wastewater. In CWs, microorganisms play a major role in the degradation of organic pollutants but the concentration of nutrients, surfactant, and aeration (NSA) in oil-contaminated water is one of the factors that affect the persistence and metabolic functioning of hydrocarbon-degrading microorganisms. In the present investigation, the influence of the addition of NSA on the persistence of the augmented bacteria, copy of (alkane hydroxylase gene) alkB gene, and its expression level in the water, soil, and plants of CWs were evaluated. The CWs mesocosms were developed by the vegetation of Typha latifolia and Cyperus laevigatus and inoculated with the bacterial consortium (Pseudomonas putida TYRI39, Acinetobacter junii TYRH47, Acinetobacter sp. CYRH17, Pseudomonas sp. CYSI27, and Pseudomonas sp. TYRH42). The mesocosms were provided with nutrients (20 mg l− 1 N, 2.6 mg l− 1 P, and 16.4 mg l− 1 K) in liquid form, surfactant Tween-20 (0.2%, v/v) in liquid form, and aeration (≥ 7.0 ± 1 mg l− 1 DO) using aeration pump. The addition of NSA in CWs enhanced the persistence and metabolic functioning of the inoculated bacteria in the water, rhizospheric soil, and plants. The maximum hydrocarbon removal (97%) was observed in the water treated by CWs having C. laevigatus, bacteria, and NSA, and it is correlated with the copy numbers of alkB and its expression level. The application of NSA in CWs not only improved bacterial persistence and catabolic gene expression but also increased plant development and hydrocarbon removal.

KW - Bacterial persistence

KW - Constructed wetlands

KW - Crude oil

KW - Gene abundance and expression

KW - Metabolic activity

UR - http://www.scopus.com/inward/record.url?scp=85190787034&partnerID=8YFLogxK

U2 - 10.1186/s40643-024-00757-5

DO - 10.1186/s40643-024-00757-5

M3 - Journal article

AN - SCOPUS:85190787034

SN - 2197-4365

VL - 11

JO - Bioresources and Bioprocessing

JF - Bioresources and Bioprocessing

IS - 1

M1 - 40

ER -

Nutrients, surfactants, and aeration in constructed wetlands affect bacterial persistence and metabolic activity during the remediation of crude oil-contaminated water

Abstract

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