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Nuclear pore complex assembly and maintenance in POM121- and gp210-deficient cells.

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Standard

Nuclear pore complex assembly and maintenance in POM121- and gp210-deficient cells. / Stavru, Fabrizia; Nautrup-Pedersen, Gitte; Cordes, Volker C; Görlich, Dirk.

I: Journal of Cell Biology, Bind 173, Nr. 4, 2006, s. 477-83.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Stavru, F, Nautrup-Pedersen, G, Cordes, VC & Görlich, D 2006, 'Nuclear pore complex assembly and maintenance in POM121- and gp210-deficient cells.', Journal of Cell Biology, bind 173, nr. 4, s. 477-83. https://doi.org/10.1083/jcb.200601002

APA

Stavru, F., Nautrup-Pedersen, G., Cordes, V. C., & Görlich, D. (2006). Nuclear pore complex assembly and maintenance in POM121- and gp210-deficient cells. Journal of Cell Biology, 173(4), 477-83. https://doi.org/10.1083/jcb.200601002

CBE

Stavru F, Nautrup-Pedersen G, Cordes VC, Görlich D. 2006. Nuclear pore complex assembly and maintenance in POM121- and gp210-deficient cells. Journal of Cell Biology. 173(4):477-83. https://doi.org/10.1083/jcb.200601002

MLA

Vancouver

Stavru F, Nautrup-Pedersen G, Cordes VC, Görlich D. Nuclear pore complex assembly and maintenance in POM121- and gp210-deficient cells. Journal of Cell Biology. 2006;173(4):477-83. https://doi.org/10.1083/jcb.200601002

Author

Stavru, Fabrizia ; Nautrup-Pedersen, Gitte ; Cordes, Volker C ; Görlich, Dirk. / Nuclear pore complex assembly and maintenance in POM121- and gp210-deficient cells. I: Journal of Cell Biology. 2006 ; Bind 173, Nr. 4. s. 477-83.

Bibtex

@article{8ac12330e50611dc9afb000ea68e967b,
title = "Nuclear pore complex assembly and maintenance in POM121- and gp210-deficient cells.",
abstract = "So far, POM121 and gp210 are the only known anchoring sites of vertebrate nuclear pore complexes (NPCs) within the lipid bilayer of the nuclear envelope (NE) and, thus, are excellent candidates for initiating the NPC assembly process. Indeed, we demonstrate that POM121 can recruit several nucleoporins, such as Nup62 or Nup358, to ectopic assembly sites. It thus appears to act as a nucleation site for the assembly of NPC substructures. Nonetheless, we observed functional NPCs and intact NEs in severely POM121-depleted cells. Double knockdowns of gp210 and POM121 in HeLa cells, as well as depletion of POM121 from human fibroblasts, which do not express gp210, further suggest that NPCs can assemble or at least persist in a POM121- and gp210-free form. This points to extensive redundancies in protein-protein interactions within NPCs and suggests that vertebrate NPCs contain additional membrane-integral nucleoporins for anchorage within the lipid bilayer of the NE. In Stavru et al., we describe such an additional transmembrane nucleoporin as the metazoan orthologue of yeast Ndc1p. Udgivelsesdato: 2006-May-22",
keywords = "Fibroblasts, Hela Cells, Humans, Membrane Glycoproteins, Membrane Lipids, Molecular Chaperones, Nuclear Pore, Nuclear Pore Complex Proteins, Nuclear Proteins, RNA Interference",
author = "Fabrizia Stavru and Gitte Nautrup-Pedersen and Cordes, {Volker C} and Dirk G{\"o}rlich",
year = "2006",
doi = "10.1083/jcb.200601002",
language = "English",
volume = "173",
pages = "477--83",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "4",

}

RIS

TY - JOUR

T1 - Nuclear pore complex assembly and maintenance in POM121- and gp210-deficient cells.

AU - Stavru, Fabrizia

AU - Nautrup-Pedersen, Gitte

AU - Cordes, Volker C

AU - Görlich, Dirk

PY - 2006

Y1 - 2006

N2 - So far, POM121 and gp210 are the only known anchoring sites of vertebrate nuclear pore complexes (NPCs) within the lipid bilayer of the nuclear envelope (NE) and, thus, are excellent candidates for initiating the NPC assembly process. Indeed, we demonstrate that POM121 can recruit several nucleoporins, such as Nup62 or Nup358, to ectopic assembly sites. It thus appears to act as a nucleation site for the assembly of NPC substructures. Nonetheless, we observed functional NPCs and intact NEs in severely POM121-depleted cells. Double knockdowns of gp210 and POM121 in HeLa cells, as well as depletion of POM121 from human fibroblasts, which do not express gp210, further suggest that NPCs can assemble or at least persist in a POM121- and gp210-free form. This points to extensive redundancies in protein-protein interactions within NPCs and suggests that vertebrate NPCs contain additional membrane-integral nucleoporins for anchorage within the lipid bilayer of the NE. In Stavru et al., we describe such an additional transmembrane nucleoporin as the metazoan orthologue of yeast Ndc1p. Udgivelsesdato: 2006-May-22

AB - So far, POM121 and gp210 are the only known anchoring sites of vertebrate nuclear pore complexes (NPCs) within the lipid bilayer of the nuclear envelope (NE) and, thus, are excellent candidates for initiating the NPC assembly process. Indeed, we demonstrate that POM121 can recruit several nucleoporins, such as Nup62 or Nup358, to ectopic assembly sites. It thus appears to act as a nucleation site for the assembly of NPC substructures. Nonetheless, we observed functional NPCs and intact NEs in severely POM121-depleted cells. Double knockdowns of gp210 and POM121 in HeLa cells, as well as depletion of POM121 from human fibroblasts, which do not express gp210, further suggest that NPCs can assemble or at least persist in a POM121- and gp210-free form. This points to extensive redundancies in protein-protein interactions within NPCs and suggests that vertebrate NPCs contain additional membrane-integral nucleoporins for anchorage within the lipid bilayer of the NE. In Stavru et al., we describe such an additional transmembrane nucleoporin as the metazoan orthologue of yeast Ndc1p. Udgivelsesdato: 2006-May-22

KW - Fibroblasts

KW - Hela Cells

KW - Humans

KW - Membrane Glycoproteins

KW - Membrane Lipids

KW - Molecular Chaperones

KW - Nuclear Pore

KW - Nuclear Pore Complex Proteins

KW - Nuclear Proteins

KW - RNA Interference

U2 - 10.1083/jcb.200601002

DO - 10.1083/jcb.200601002

M3 - Journal article

C2 - 16702234

VL - 173

SP - 477

EP - 483

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 4

ER -