Novel fluorescent nanoparticles for ultrasensitive identification of nucleic acids by optical methods

Publikation: Bidrag til bog/antologi/rapport/proceedingKonferencebidrag i proceedingsForskningpeer review

  • Mads Westergaard Mulberg, Syddansk Universitet
  • ,
  • Maria Taskova, Syddansk Universitet
  • ,
  • Rasmus P. Thomsen
  • ,
  • Anders H. Okholm
  • ,
  • Jørgen Kjems
  • Kira Astakhova, Syddansk Universitet

For decades, the detection of nucleic acids and their interactions at low abundances has been a challenging task. Present nucleic acid diagnostics are primarily based on enzymatic reactions including sequencing, polymerase-chain reaction and microarrays. However, the use of enzymatic amplification interferes with the initial biomolecular system, is limited to in vitro assays, often time consuming and rather expensive. Therefore, there is interest in new amplification-free detection methods. A tremendous progress has been made in fluorescence based optical detection of biomolecules. In this work, we aimed at developing efficient tools for amplification-free nucleic acid detection. The result of simple and inexpensive polymerization in the presence of fluorescent dyes and additional functionalization reagents was ultra-bright fluorescent nanoparticles modified with additional groups for bioconjugation.

OriginalsprogEngelsk
TitelBiotech, Biomaterials and Biomedical - TechConnect Briefs 2017
Antal sider4
Vol/bind3
ForlagTechConnect
Udgivelsesår1 jan. 2017
Sider118-121
ISBN (Elektronisk)9780998878201
StatusUdgivet - 1 jan. 2017
Begivenhed11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference - Washington, USA
Varighed: 14 maj 201717 maj 2017

Konference

Konference11th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 20th Annual Nanotech Conference and Expo, and the 2017 National SBIR/STTR Conference
LandUSA
ByWashington
Periode14/05/201717/05/2017

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