TY - JOUR
T1 - No evidence of increased anti-M3 muscarinic acetylcholine receptor autoantibodies in SSA-positive connective tissue disease patients
AU - Hatipoğlu, Elif
AU - Mikkelsen, Jakob Hauge
AU - Korsholm, Trine Line
AU - Hvid, Malene
AU - Deleuran, Bent
AU - Dahl, Marie Louise Næstholt
N1 - Publisher Copyright:
© 2023 Scandinavian Societies for Pathology, Medical Microbiology and Immunology.
PY - 2023/10
Y1 - 2023/10
N2 - Anti-muscarinic type 3 receptor autoantibodies (M3R) and anti-SSA antibodies are both related to salivary secretion. The presence of M3R antibodies in Sjögren's syndrome is previously demonstrated; nevertheless, the relationship between the anti-SSA antibodies and M3R fragment antibodies, namely the N terminal, first, second, and third extracellular loops, remains to be elucidated. In this study, we analyzed the antibodies against the M3R epitopes in healthy controls and anti-SSA antibody-positive connective tissue disease patients through ELISA method. Antibodies against the first, second, and third extracellular loop (M3R211–230) were not increased in anti-SSA positive patients compared to healthy controls. Indeed, antibodies against the N terminal (M3R1–33) were found to be high in healthy controls. High levels of M3R1–33 in healthy controls are a novel original finding; further research is needed for the clinical significance. There is no significant difference between SSA-positive patients and healthy controls in terms of autoantibodies against the remainder of the linear M3R fragments.
AB - Anti-muscarinic type 3 receptor autoantibodies (M3R) and anti-SSA antibodies are both related to salivary secretion. The presence of M3R antibodies in Sjögren's syndrome is previously demonstrated; nevertheless, the relationship between the anti-SSA antibodies and M3R fragment antibodies, namely the N terminal, first, second, and third extracellular loops, remains to be elucidated. In this study, we analyzed the antibodies against the M3R epitopes in healthy controls and anti-SSA antibody-positive connective tissue disease patients through ELISA method. Antibodies against the first, second, and third extracellular loop (M3R211–230) were not increased in anti-SSA positive patients compared to healthy controls. Indeed, antibodies against the N terminal (M3R1–33) were found to be high in healthy controls. High levels of M3R1–33 in healthy controls are a novel original finding; further research is needed for the clinical significance. There is no significant difference between SSA-positive patients and healthy controls in terms of autoantibodies against the remainder of the linear M3R fragments.
KW - Anti-M3R
KW - anti-SSA
KW - connective tissue disease
KW - immunology
KW - muscarinic receptor type 3
UR - http://www.scopus.com/inward/record.url?scp=85169112223&partnerID=8YFLogxK
U2 - 10.1111/apm.13349
DO - 10.1111/apm.13349
M3 - Journal article
C2 - 37620885
AN - SCOPUS:85169112223
SN - 0903-4641
VL - 131
SP - 552
EP - 555
JO - APMIS
JF - APMIS
IS - 10
ER -