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NHE-1 sequence and expression in toad, snake and fish red blood cells

Publikation: KonferencebidragPosterForskning

  • Steffen Nyegaard Thomsen, Danmark
  • Tobias Wang
  • Torsten Kristensen, Danmark
  • Molekylærbiologisk Institut
  • Zoofysiologi, Biologisk Institut
Red blood cells (RBC) from reptiles appear not to express regulatory volume increase (RVI) upon shrinkage (Kristensen et al., 2008). In other vertebrates, the RVI response is primarily mediated by activation of the Na+/H+ exchanger (NHE-1) and we, therefore decided to investigate whether red cells of reptiles express a different NHE-1 that responds less to volume activation compared to other vertebrates or simply lack the Na+/H+ exchanger. Using various tissues from the ball python (Python regius), Cane toad (Bufo marinus) and European perch (Perca fluviatilis), cDNA libraries were created and sequenced using PCR with primers generated from a clustal alignment of available NHE-1 sequences in the NR database. Having established the presence of the NHE-1 gene in python, cane toad and perch, expression was quantified by Western Blot and Immunoconfocal microscopy using fluorophore coupled NHE-1 antibodies. These studies showed that NHE-1 is expressed in RBCs from both toads and teleosts fish, but is not expressed in the RBCs of pythons. Thus, the lack of the RVI response in pythons is likely to reflect the lack of capacity to exchange Na+ and H+ for volume regulation.
OriginalsprogEngelsk
Udgivelsesår2010
Antal sider1
StatusUdgivet - 2010
Begivenhed42nd Sandbjerg meeting on membrane transport - Sønderborg, Danmark
Varighed: 19 maj 201021 maj 2010

Konference

Konference42nd Sandbjerg meeting on membrane transport
LandDanmark
BySønderborg
Periode19/05/201021/05/2010

    Forskningsområder

  • NHE-1, SLC9A1, Na-H transport, Volumenregulering, pH regulering

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