New methods for quantification of amoxicillin and clindamycin in human plasma using HPLC with UV detection.

TY - JOUR

T1 - New methods for quantification of amoxicillin and clindamycin in human plasma using HPLC with UV detection.

AU - Greibe, Eva

AU - Moser, Claus Ernst

AU - Bruun, Niels Eske

AU - Hoffmann-Lücke, Elke

PY - 2022/9/1

Y1 - 2022/9/1

N2 - Objectives: We aimed to develop simple and rapid HPLC methods for determination of amoxicillin and clindamycin in human plasma. Methods: Plasma samples were pretreated by direct deproteinization with acetonitrile and the analytical separation took place on a reverse phase Poroshell 120 EC-C18 column (2.7 μm, 2.1 × 100 mm) with a gradient of acetonitrile. UV detection at 229 nm for amoxicillin and 204 nm for clindamycin was used for determination of the antibiotics in plasma. Results: The calibration curves were linear over the concentration ranges of 1-100 mg/L for amoxicillin and 1-15 mg/L for clindamycin with a correlation coefficient of ≥0.98. Intra-assay precisions were all ≤15% and the accuracies were within ±15%. The limit of quantification (LOQ) was found to be 0.5 mg/L for amoxicillin and 1 mg/L for clindamycin with inter-assay imprecision coefficient of variances (CVs) of 18.7% and 15.6%, respectively. The present HPLC methods were successfully applied on spike-in samples and on plasma samples collected 4-6 and 3.5-5.5 h after oral antibiotic administration of 500 mg of amoxicillin and 600 mg of clindamycin, respectively. Conclusions: We have developed HPLC methods with UV detection for quantification of amoxicillin and clindamycin in human plasma. The methods are fast, simple and suitable for use in routine settings and clinical studies.

AB - Objectives: We aimed to develop simple and rapid HPLC methods for determination of amoxicillin and clindamycin in human plasma. Methods: Plasma samples were pretreated by direct deproteinization with acetonitrile and the analytical separation took place on a reverse phase Poroshell 120 EC-C18 column (2.7 μm, 2.1 × 100 mm) with a gradient of acetonitrile. UV detection at 229 nm for amoxicillin and 204 nm for clindamycin was used for determination of the antibiotics in plasma. Results: The calibration curves were linear over the concentration ranges of 1-100 mg/L for amoxicillin and 1-15 mg/L for clindamycin with a correlation coefficient of ≥0.98. Intra-assay precisions were all ≤15% and the accuracies were within ±15%. The limit of quantification (LOQ) was found to be 0.5 mg/L for amoxicillin and 1 mg/L for clindamycin with inter-assay imprecision coefficient of variances (CVs) of 18.7% and 15.6%, respectively. The present HPLC methods were successfully applied on spike-in samples and on plasma samples collected 4-6 and 3.5-5.5 h after oral antibiotic administration of 500 mg of amoxicillin and 600 mg of clindamycin, respectively. Conclusions: We have developed HPLC methods with UV detection for quantification of amoxicillin and clindamycin in human plasma. The methods are fast, simple and suitable for use in routine settings and clinical studies.

KW - CLAVULANIC ACID

UR - http://www.scopus.com/inward/record.url?scp=85144567431&partnerID=8YFLogxK

U2 - 10.1093/jac/dkac195

DO - 10.1093/jac/dkac195

M3 - Journal article

C2 - 35733365

SN - 0305-7453

VL - 77

SP - 2437

EP - 2440

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

IS - 9

M1 - dkac195

ER -