Neuronal and glial responses to siRNA-coated nerve guide implants in vitro

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Neuronal and glial responses to siRNA-coated nerve guide implants in vitro. / Hoffmann, Nadine; Mittnacht, Ursula; Hartmann, Hanna; Baumer, Yvonne; Kjems, Jørgen; Oberhoffner, Sven; Schlosshauer, Burkhard.

I: Neuroscience Letters, Bind 494, Nr. 1, 20.04.2011, s. 14-8.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Hoffmann, N, Mittnacht, U, Hartmann, H, Baumer, Y, Kjems, J, Oberhoffner, S & Schlosshauer, B 2011, 'Neuronal and glial responses to siRNA-coated nerve guide implants in vitro', Neuroscience Letters, bind 494, nr. 1, s. 14-8. https://doi.org/10.1016/j.neulet.2011.02.043

APA

Hoffmann, N., Mittnacht, U., Hartmann, H., Baumer, Y., Kjems, J., Oberhoffner, S., & Schlosshauer, B. (2011). Neuronal and glial responses to siRNA-coated nerve guide implants in vitro. Neuroscience Letters, 494(1), 14-8. https://doi.org/10.1016/j.neulet.2011.02.043

CBE

Hoffmann N, Mittnacht U, Hartmann H, Baumer Y, Kjems J, Oberhoffner S, Schlosshauer B. 2011. Neuronal and glial responses to siRNA-coated nerve guide implants in vitro. Neuroscience Letters. 494(1):14-8. https://doi.org/10.1016/j.neulet.2011.02.043

MLA

Vancouver

Hoffmann N, Mittnacht U, Hartmann H, Baumer Y, Kjems J, Oberhoffner S o.a. Neuronal and glial responses to siRNA-coated nerve guide implants in vitro. Neuroscience Letters. 2011 apr 20;494(1):14-8. https://doi.org/10.1016/j.neulet.2011.02.043

Author

Hoffmann, Nadine ; Mittnacht, Ursula ; Hartmann, Hanna ; Baumer, Yvonne ; Kjems, Jørgen ; Oberhoffner, Sven ; Schlosshauer, Burkhard. / Neuronal and glial responses to siRNA-coated nerve guide implants in vitro. I: Neuroscience Letters. 2011 ; Bind 494, Nr. 1. s. 14-8.

Bibtex

@article{355fc15d993c487796efbbb7dcfda7d7,
title = "Neuronal and glial responses to siRNA-coated nerve guide implants in vitro",
abstract = "The manipulation of gene expression by RNA interference could play a key role in future neurotherapies, for example in the development of biohydrid implants to bridge nerve and spinal cord lesion gaps. Such resorbable biomaterial prostheses could serve as growth substrates together with specific siRNA to foster neuronal regeneration. To the best of our knowledge, we are the first to biofunctionalize neuronal prostheses with siRNA. We analyzed neuronal and Schwann cell responses to scrambled siRNA coated polydioxanone polymer filaments designed to imitate pro-regenerative bands of B{\"u}ngner for oriented axonal regrowth. With a view to future clinical applications we were especially interested in potentially detrimental side effects. We employed a variety of in vitro methods, including a novel impedance electrode microchamber assay, fluorescence and scanning electron microscopy, metabolic labeling and RT-PCR. We found that the application of chitosan/siRNA nanoparticles (1) did not affect glial cell motility or (2) axonal growth in contrast to other formulations, (3) only slightly reduced proliferation, and (4) did not induce inflammatory responses that might hamper axonal regeneration. The data suggest that chitosan/siRNA nanoparticle-coated polymer filaments are suitable for use in biohybrid implants with no significant side effects on neuronal and glial cells.",
keywords = "Analysis of Variance, Animals, Axons, Biocompatible Materials, Chitosan, Immunohistochemistry, Nanoparticles, Neurons, RNA, Small Interfering, Rats, Rats, Inbred Lew, Schwann Cells",
author = "Nadine Hoffmann and Ursula Mittnacht and Hanna Hartmann and Yvonne Baumer and J{\o}rgen Kjems and Sven Oberhoffner and Burkhard Schlosshauer",
note = "Copyright {\textcopyright} 2011 Elsevier Ireland Ltd. All rights reserved.",
year = "2011",
month = apr,
day = "20",
doi = "10.1016/j.neulet.2011.02.043",
language = "English",
volume = "494",
pages = "14--8",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd.",
number = "1",

}

RIS

TY - JOUR

T1 - Neuronal and glial responses to siRNA-coated nerve guide implants in vitro

AU - Hoffmann, Nadine

AU - Mittnacht, Ursula

AU - Hartmann, Hanna

AU - Baumer, Yvonne

AU - Kjems, Jørgen

AU - Oberhoffner, Sven

AU - Schlosshauer, Burkhard

N1 - Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.

PY - 2011/4/20

Y1 - 2011/4/20

N2 - The manipulation of gene expression by RNA interference could play a key role in future neurotherapies, for example in the development of biohydrid implants to bridge nerve and spinal cord lesion gaps. Such resorbable biomaterial prostheses could serve as growth substrates together with specific siRNA to foster neuronal regeneration. To the best of our knowledge, we are the first to biofunctionalize neuronal prostheses with siRNA. We analyzed neuronal and Schwann cell responses to scrambled siRNA coated polydioxanone polymer filaments designed to imitate pro-regenerative bands of Büngner for oriented axonal regrowth. With a view to future clinical applications we were especially interested in potentially detrimental side effects. We employed a variety of in vitro methods, including a novel impedance electrode microchamber assay, fluorescence and scanning electron microscopy, metabolic labeling and RT-PCR. We found that the application of chitosan/siRNA nanoparticles (1) did not affect glial cell motility or (2) axonal growth in contrast to other formulations, (3) only slightly reduced proliferation, and (4) did not induce inflammatory responses that might hamper axonal regeneration. The data suggest that chitosan/siRNA nanoparticle-coated polymer filaments are suitable for use in biohybrid implants with no significant side effects on neuronal and glial cells.

AB - The manipulation of gene expression by RNA interference could play a key role in future neurotherapies, for example in the development of biohydrid implants to bridge nerve and spinal cord lesion gaps. Such resorbable biomaterial prostheses could serve as growth substrates together with specific siRNA to foster neuronal regeneration. To the best of our knowledge, we are the first to biofunctionalize neuronal prostheses with siRNA. We analyzed neuronal and Schwann cell responses to scrambled siRNA coated polydioxanone polymer filaments designed to imitate pro-regenerative bands of Büngner for oriented axonal regrowth. With a view to future clinical applications we were especially interested in potentially detrimental side effects. We employed a variety of in vitro methods, including a novel impedance electrode microchamber assay, fluorescence and scanning electron microscopy, metabolic labeling and RT-PCR. We found that the application of chitosan/siRNA nanoparticles (1) did not affect glial cell motility or (2) axonal growth in contrast to other formulations, (3) only slightly reduced proliferation, and (4) did not induce inflammatory responses that might hamper axonal regeneration. The data suggest that chitosan/siRNA nanoparticle-coated polymer filaments are suitable for use in biohybrid implants with no significant side effects on neuronal and glial cells.

KW - Analysis of Variance

KW - Animals

KW - Axons

KW - Biocompatible Materials

KW - Chitosan

KW - Immunohistochemistry

KW - Nanoparticles

KW - Neurons

KW - RNA, Small Interfering

KW - Rats

KW - Rats, Inbred Lew

KW - Schwann Cells

U2 - 10.1016/j.neulet.2011.02.043

DO - 10.1016/j.neulet.2011.02.043

M3 - Journal article

C2 - 21352894

VL - 494

SP - 14

EP - 18

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 1

ER -