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Negative regulation of CCaMK is essential for symbiotic infection

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  • Jinqiu Liao, Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, Canada
  • Sylvia Singh, Biocenter University of Munich, Department of Genetics, Tyskland
  • Md S Hossain, Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, Canada
  • Stig Uggerhøj Andersen
  • Loretta Ross, Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, Canada
  • Dario Bonetta, Institute of Technology, University of Ontario, Canada
  • Yonghong Zhou, Triticeae Research Institute, Sichuan Agricultural University, Kina
  • Shusei Sato, Kazusa DNA Research Institute, Japan
  • Satoshi Tabata, Kazusa DNA Research Institute, Japan
  • Jens Stougaard
  • Krzysztof Szczyglowski, Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, Canada
  • Martin Parniske, Biocenter University of Munich, Tyskland
One of the earliest responses of legumes to symbiotic signalling is oscillation of the calcium concentration in the nucleoplasm of root epidermal cells. Integration and decoding of the calcium-spiking signal involve a calcium- and calmodulin-dependent protein kinase (CCaMK) and its phosphorylation substrates, such as CYCLOPS. Here we describe the Lotus japonicus ccamk-14 mutant that originated from a har1-1 suppressor screen. The ccamk-14 mutation causes a serine to asparagine substitution at position 337 located within the calmodulin binding site, which we determined to be an in vitro phosphorylation site in CCaMK. We show that ccamk-14 exerts cell-specific effects on symbiosis. The mutant is characterized by an increased frequency of epidermal infections and significantly compromised cortical infections by Mesorhizobium loti and also the arbuscular mycorrhiza fungus Rhizophagus irregularis. The S337 residue is conserved across angiosperm CCaMKs, and testing discrete substitutions at this site showed that it participates in a negative regulation of CCaMK activity, which is required for the cell-type-specific integration of symbiotic signalling.
OriginalsprogEngelsk
TidsskriftPlant Journal
Vol/bind72
Nummer4
Sider (fra-til)572-584
Antal sider12
ISSN0960-7412
DOI
StatusUdgivet - nov. 2012

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