N4-acetylcytidine (ac4C) promotes mRNA localization to stress granules

TY - JOUR

T1 - N4-acetylcytidine (ac4C) promotes mRNA localization to stress granules

AU - Kudrin, Pavel

AU - Singh, Ankita

AU - Meierhofer, David

AU - Kuśnierczyk, Anna

AU - Ørom, Ulf Andersson Vang

N1 - Publisher Copyright: © The Author(s) 2024.

PY - 2024/4

Y1 - 2024/4

N2 - Stress granules are an integral part of the stress response that are formed from non-translating mRNAs aggregated with proteins. While much is known about stress granules, the factors that drive their mRNA localization are incompletely described. Modification of mRNA can alter the properties of the nucleobases and affect processes such as translation, splicing and localization of individual transcripts. Here, we show that the RNA modification N4-acetylcytidine (ac4C) on mRNA associates with transcripts enriched in stress granules and that stress granule localized transcripts with ac4C are specifically translationally regulated. We also show that ac4C on mRNA can mediate localization of the protein NOP58 to stress granules. Our results suggest that acetylation of mRNA regulates localization of both stress-sensitive transcripts and RNA-binding proteins to stress granules and adds to our understanding of the molecular mechanisms responsible for stress granule formation.

AB - Stress granules are an integral part of the stress response that are formed from non-translating mRNAs aggregated with proteins. While much is known about stress granules, the factors that drive their mRNA localization are incompletely described. Modification of mRNA can alter the properties of the nucleobases and affect processes such as translation, splicing and localization of individual transcripts. Here, we show that the RNA modification N4-acetylcytidine (ac4C) on mRNA associates with transcripts enriched in stress granules and that stress granule localized transcripts with ac4C are specifically translationally regulated. We also show that ac4C on mRNA can mediate localization of the protein NOP58 to stress granules. Our results suggest that acetylation of mRNA regulates localization of both stress-sensitive transcripts and RNA-binding proteins to stress granules and adds to our understanding of the molecular mechanisms responsible for stress granule formation.

KW - mRNA Localization

KW - RNA Acetylation

KW - Stress Granules

KW - Translation

KW - RNA-Binding Proteins/metabolism

KW - Cytidine/genetics

KW - RNA, Messenger/genetics

UR - http://www.scopus.com/inward/record.url?scp=85185962795&partnerID=8YFLogxK

U2 - 10.1038/s44319-024-00098-6

DO - 10.1038/s44319-024-00098-6

M3 - Journal article

C2 - 38413733

AN - SCOPUS:85185962795

SN - 1469-221X

VL - 25

SP - 1814

EP - 1834

JO - EMBO Reports

JF - EMBO Reports

IS - 4

ER -