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Mutation of Gly717Phe in human topoisomerase 1B has an effect on enzymatic function, reactivity to the camptothecin anticancer drug and on the linker domain orientation

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  • Zhenxing Wang, Univeristy of Rome, Tor Vergata, Italien
  • Ilda D'Annessa, Univeristy of Rome, Tor Vergata, Italien
  • Cinzia Tesauro
  • Stefano Croce, Univeristy of Rome, Tor Vergata, Italien
  • Alessio Ottaviani, Univeristy of Rome, Tor Vergata, Italien
  • Paola Fiorani, University of Rome, Tor Vergata, Italien
  • Alessandro Desideri, University of Rome, Tor Vergata, Italien
Human topoisomerase 1B controls the topological state of supercoiled DNA allowing the progression of fundamental cellular processes. The enzyme, which is the unique molecular target of the natural anticancer compound camptothecin, acts by cleaving one DNA strand and forming a transient protein–DNA covalent adduct. In this work the role of the Gly717 residue, located in a α-helix structure bridging the active site and the linker domain, has been investigated mutating it in Phe. The mutation gives rise to drug resistance in vivo as observed through a viability assay of yeast cells. In vitro activity assays show that the mutant is characterized by a fast religation rate, only partially reduced by the presence of the drug. Comparative molecular dynamics simulations of the native and mutant proteins indicate that the mutation of Gly717 affects the motion orientation of the linker domain, changing its interaction with the DNA substrate, likely affecting the strand rotation and religation rate. The mutation also causes a slight rearrangement of the active site and of the drug binding site, providing an additional explanation for the lowered effect of camptothecin toward the mutant.
OriginalsprogEngelsk
TidsskriftBBA Proteins and Proteomics
Vol/bind1854
Nummer8
Sider (fra-til)860-868
Antal sider9
ISSN1570-9639
DOI
StatusUdgivet - aug. 2015

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