Multiple site-directed mutagenesis via simple cloning by prolonged overlap extension

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    Abstract

    We describe the application of simple cloning by prolonged overlap extension for multiple site-directed mutagenesis in the same plasmid. We show that it is possible to use this technique with very short PCR templates. The technique is ideally suited for the generation of longer donor DNA sequences for CRISPR/Cas9-mediated homologous repair.

    OriginalsprogEngelsk
    TidsskriftBioTechniques
    Vol/bind68
    Nummer6
    Sider (fra-til)345-348
    Antal sider4
    ISSN0736-6205
    DOI
    StatusUdgivet - jun. 2020

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