Multi-method research strategy for understanding changes in storage protein composition in developing barley grain to improve nutritional profile

Publikation: Bog/antologi/afhandling/rapportPh.d.-afhandlingForskning

  • Agnieszka Ewa Kaczmarczyk, Danmark

Barley (Hordeum vulgare L.) is cultivated in a range of diverse environments and is widely utilised as feed for animal and as malt in brewing. Nitrogen (N) is a key macronutrient whch directly increases plant growth and is used as a fertiliser to meet the demands for higher yield. However, the increases in the nitrogen fertiliser are not accompanied by a good quallity of protein composition in barley grain. Hodeins are coded by highly polymorphic genes which are clustered into four main families. B.C.D and γ. The high N-dosage promotes C-hordein synthesis, the class with the lowest content of essential amino acids (AAs) (lysine, methionine) and the highest content of non-essential AAs (proline, glutamine).
The key objective of the present work was to get a detailed insight into the developmental expression of the storage protein of field grown barley grain in relation to different nitrogen regimes. To reach the objective, integrated transcriptomics and proteomics analysis complemented with AAs profiling have been undertaken.

OriginalsprogEngelsk
UdgivelsesstedDepartment of Molecular Biology and Genetics
ForlagAarhus University, Science and Technology
Antal sider178
ISBN (Trykt)978-87-92936-65-3
Rekvirerende organGraduate School of Science and Technology
StatusUdgivet - 29 apr. 2013

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