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MRX protects fork integrity at protein–DNA barriers, and its absence causes checkpoint activation dependent on chromatin context

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MRX protects fork integrity at protein–DNA barriers, and its absence causes checkpoint activation dependent on chromatin context. / Bentsen, Iben Bach; Nielsen, Ida; Lisby, Michael; Nielsen, Helena Berner Nedergaard; Sen Gupta, Souvik; Mundbjerg, Kamilla; Andersen, Anni Hangaard; Bjergbæk, Lotte.

I: Nucleic Acids Research, Bind 41, Nr. 5, 01.02.2013, s. 3173-3189.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Bentsen, IB, Nielsen, I, Lisby, M, Nielsen, HBN, Sen Gupta, S, Mundbjerg, K, Andersen, AH & Bjergbæk, L 2013, 'MRX protects fork integrity at protein–DNA barriers, and its absence causes checkpoint activation dependent on chromatin context', Nucleic Acids Research, bind 41, nr. 5, s. 3173-3189. https://doi.org/10.1093/nar/gkt051

APA

Bentsen, I. B., Nielsen, I., Lisby, M., Nielsen, H. B. N., Sen Gupta, S., Mundbjerg, K., Andersen, A. H., & Bjergbæk, L. (2013). MRX protects fork integrity at protein–DNA barriers, and its absence causes checkpoint activation dependent on chromatin context. Nucleic Acids Research, 41(5), 3173-3189. https://doi.org/10.1093/nar/gkt051

CBE

MLA

Vancouver

Bentsen IB, Nielsen I, Lisby M, Nielsen HBN, Sen Gupta S, Mundbjerg K o.a. MRX protects fork integrity at protein–DNA barriers, and its absence causes checkpoint activation dependent on chromatin context. Nucleic Acids Research. 2013 feb 1;41(5):3173-3189. https://doi.org/10.1093/nar/gkt051

Author

Bentsen, Iben Bach ; Nielsen, Ida ; Lisby, Michael ; Nielsen, Helena Berner Nedergaard ; Sen Gupta, Souvik ; Mundbjerg, Kamilla ; Andersen, Anni Hangaard ; Bjergbæk, Lotte. / MRX protects fork integrity at protein–DNA barriers, and its absence causes checkpoint activation dependent on chromatin context. I: Nucleic Acids Research. 2013 ; Bind 41, Nr. 5. s. 3173-3189.

Bibtex

@article{71e2321b450b487ba8e9730651936bbc,
title = "MRX protects fork integrity at protein–DNA barriers, and its absence causes checkpoint activation dependent on chromatin context",
abstract = "To address how eukaryotic replication forks respond to fork stalling caused by strong non-covalent protein–DNA barriers, we engineered the controllable Fob-block system in Saccharomyces cerevisiae. This system allows us to strongly induce and control replication fork barriers (RFB) at their natural location within the rDNA. We discover a pivotal role for the MRX (Mre11, Rad50, Xrs2) complex for fork integrity at RFBs, which differs from its acknowledged function in double-strand break processing. Consequently, in the absence of the MRX complex, single-stranded DNA (ssDNA) accumulates at the rDNA. Based on this, we propose a model where the MRX complex specifically protects stalled forks at protein–DNA barriers, and its absence leads to processing resulting in ssDNA. To our surprise, this ssDNA does not trigger a checkpoint response. Intriguingly, however, placing RFBs ectopically on chromosome VI provokes a strong Rad53 checkpoint activation in the absence of Mre11. We demonstrate that proper checkpoint signalling within the rDNA is restored on deletion of SIR2. This suggests the surprising and novel co",
author = "Bentsen, {Iben Bach} and Ida Nielsen and Michael Lisby and Nielsen, {Helena Berner Nedergaard} and {Sen Gupta}, Souvik and Kamilla Mundbjerg and Andersen, {Anni Hangaard} and Lotte Bjergb{\ae}k",
year = "2013",
month = feb,
day = "1",
doi = "10.1093/nar/gkt051",
language = "English",
volume = "41",
pages = "3173--3189",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "5",

}

RIS

TY - JOUR

T1 - MRX protects fork integrity at protein–DNA barriers, and its absence causes checkpoint activation dependent on chromatin context

AU - Bentsen, Iben Bach

AU - Nielsen, Ida

AU - Lisby, Michael

AU - Nielsen, Helena Berner Nedergaard

AU - Sen Gupta, Souvik

AU - Mundbjerg, Kamilla

AU - Andersen, Anni Hangaard

AU - Bjergbæk, Lotte

PY - 2013/2/1

Y1 - 2013/2/1

N2 - To address how eukaryotic replication forks respond to fork stalling caused by strong non-covalent protein–DNA barriers, we engineered the controllable Fob-block system in Saccharomyces cerevisiae. This system allows us to strongly induce and control replication fork barriers (RFB) at their natural location within the rDNA. We discover a pivotal role for the MRX (Mre11, Rad50, Xrs2) complex for fork integrity at RFBs, which differs from its acknowledged function in double-strand break processing. Consequently, in the absence of the MRX complex, single-stranded DNA (ssDNA) accumulates at the rDNA. Based on this, we propose a model where the MRX complex specifically protects stalled forks at protein–DNA barriers, and its absence leads to processing resulting in ssDNA. To our surprise, this ssDNA does not trigger a checkpoint response. Intriguingly, however, placing RFBs ectopically on chromosome VI provokes a strong Rad53 checkpoint activation in the absence of Mre11. We demonstrate that proper checkpoint signalling within the rDNA is restored on deletion of SIR2. This suggests the surprising and novel co

AB - To address how eukaryotic replication forks respond to fork stalling caused by strong non-covalent protein–DNA barriers, we engineered the controllable Fob-block system in Saccharomyces cerevisiae. This system allows us to strongly induce and control replication fork barriers (RFB) at their natural location within the rDNA. We discover a pivotal role for the MRX (Mre11, Rad50, Xrs2) complex for fork integrity at RFBs, which differs from its acknowledged function in double-strand break processing. Consequently, in the absence of the MRX complex, single-stranded DNA (ssDNA) accumulates at the rDNA. Based on this, we propose a model where the MRX complex specifically protects stalled forks at protein–DNA barriers, and its absence leads to processing resulting in ssDNA. To our surprise, this ssDNA does not trigger a checkpoint response. Intriguingly, however, placing RFBs ectopically on chromosome VI provokes a strong Rad53 checkpoint activation in the absence of Mre11. We demonstrate that proper checkpoint signalling within the rDNA is restored on deletion of SIR2. This suggests the surprising and novel co

U2 - 10.1093/nar/gkt051

DO - 10.1093/nar/gkt051

M3 - Journal article

C2 - 23376930

VL - 41

SP - 3173

EP - 3189

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 5

ER -