Movies of ice-embedded particles enhance resolution in electron cryo-microscopy

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

  • Melody G Campbell, National Resource for Automated Molecular Microscopy, Department of Cell Biology, The Scripps Research Institute, La Jolla, CA 92037, USA., Danmark
  • Anchi Cheng, Danmark
  • Axel F Brilot, Danmark
  • Arne Möller, National Resource for Automated Molecular Microscopy, Department of Cell Biology, The Scripps Research Institute, La Jolla, California, USA., Danmark
  • Dmitry Lyumkis, Danmark
  • David Veesler, Danmark
  • Junhua Pan, Danmark
  • Stephen C Harrison, Danmark
  • Clinton S Potter
  • ,
  • Bridget Carragher
  • ,
  • Nikolaus Grigorieff, Danmark

Low-dose images obtained by electron cryo-microscopy (cryo-EM) are often affected by blurring caused by sample motion during electron beam exposure, degrading signal especially at high resolution. We show here that we can align frames of movies, recorded with a direct electron detector during beam exposure of rotavirus double-layered particles, thereby greatly reducing image blurring caused by beam-induced motion and sample stage instabilities. This procedure increases the efficiency of cryo-EM imaging and enhances the resolution obtained in three-dimensional reconstructions of the particle. Using movies in this way is generally applicable to all cryo-EM samples and should also improve the performance of midrange electron microscopes that may have limited mechanical stability and beam coherence.

OriginalsprogEngelsk
TidsskriftStructure
Vol/bind20
Nummer11
Sider (fra-til)1823-8
Antal sider6
ISSN0910-8270
DOI
StatusUdgivet - 7 nov. 2012
Eksternt udgivetJa

Se relationer på Aarhus Universitet Citationsformater

ID: 77176062