Morphology, proliferation, and osteogenic differentiation of mesenchymal stem cells cultured on titanium, tantalum, and chromium surfaces

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Morphology, proliferation, and osteogenic differentiation of mesenchymal stem cells cultured on titanium, tantalum, and chromium surfaces. / Stiehler, Maik; Lind, Martin; Mygind, Tina; Baatrup, Anette; Dolatshahi-Pirouz, Alireza; Li, Haisheng; Foss, Morten; Besenbacher, Flemming; Kassem, Moustapha; Bünger, Cody.

I: Journal of Biomedical Materials Research. Part A, Bind 86, Nr. 2, 2008, s. 448-58.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Stiehler, M, Lind, M, Mygind, T, Baatrup, A, Dolatshahi-Pirouz, A, Li, H, Foss, M, Besenbacher, F, Kassem, M & Bünger, C 2008, 'Morphology, proliferation, and osteogenic differentiation of mesenchymal stem cells cultured on titanium, tantalum, and chromium surfaces', Journal of Biomedical Materials Research. Part A, bind 86, nr. 2, s. 448-58. https://doi.org/10.1002/jbm.a.31602

APA

Stiehler, M., Lind, M., Mygind, T., Baatrup, A., Dolatshahi-Pirouz, A., Li, H., Foss, M., Besenbacher, F., Kassem, M., & Bünger, C. (2008). Morphology, proliferation, and osteogenic differentiation of mesenchymal stem cells cultured on titanium, tantalum, and chromium surfaces. Journal of Biomedical Materials Research. Part A, 86(2), 448-58. https://doi.org/10.1002/jbm.a.31602

CBE

MLA

Vancouver

Author

Stiehler, Maik ; Lind, Martin ; Mygind, Tina ; Baatrup, Anette ; Dolatshahi-Pirouz, Alireza ; Li, Haisheng ; Foss, Morten ; Besenbacher, Flemming ; Kassem, Moustapha ; Bünger, Cody. / Morphology, proliferation, and osteogenic differentiation of mesenchymal stem cells cultured on titanium, tantalum, and chromium surfaces. I: Journal of Biomedical Materials Research. Part A. 2008 ; Bind 86, Nr. 2. s. 448-58.

Bibtex

@article{b8658870ee0211dd8f9a000ea68e967b,
title = "Morphology, proliferation, and osteogenic differentiation of mesenchymal stem cells cultured on titanium, tantalum, and chromium surfaces",
abstract = "Metallic implants are widely used in orthopedic surgery and dentistry. Durable osseous fixation of an implant requires that osteoprogenitor cells attach and adhere to the implant, proliferate, differentiate into osteoblasts, and produce mineralized matrix. In the present study, we investigated the interactions between human mesenchymal stem cells (MSCs) and smooth surfaces of titanium (Ti), tantalum (Ta), and chromium (Cr). Mean cellular area was quantified using fluorescence microscopy (4 h). Cellular proliferation was assessed by (3)H-thymidine incorporation and methylene blue cell counting assays (4 days). Osteogenic differentiation response was quantified by cell-specific alkaline phosphatase activity (ALP) assay (4 days), expression analysis of bone-related genes (4 days), and mineralization assay (21 days). Undifferentiated and osteogenically stimulated MSCs cultured on the different surfaces showed the same tendencies for proliferation and differentiation. MSCs exposed to Ti surfaces demonstrated enhanced proliferation compared with Ta and Cr surfaces. Cultivation of MSCs on Ta surfaces resulted in significantly increased mean cellular area and cell-specific ALP activity compared with the other surfaces tested. Cells cultured on Cr demonstrated reduced spreading and proliferation. In conclusion, Ta metal, as an alternative for Ti, can be considered as a promising biocompatible material, whereas further studies are needed to fully understand the role of Cr and its alloys in bone implants.",
keywords = "Biocompatible Materials, Cell Culture Techniques, Cell Differentiation, Cell Proliferation, Cell Shape, Chromium, Dental Implants, Humans, Joint Prosthesis, Mesenchymal Stem Cells, Osteoblasts, Osteogenesis, Tantalum, Titanium",
author = "Maik Stiehler and Martin Lind and Tina Mygind and Anette Baatrup and Alireza Dolatshahi-Pirouz and Haisheng Li and Morten Foss and Flemming Besenbacher and Moustapha Kassem and Cody B{\"u}nger",
year = "2008",
doi = "10.1002/jbm.a.31602",
language = "English",
volume = "86",
pages = "448--58",
journal = "Journal of Biomedical Materials Research. Part A",
issn = "1549-3296",
publisher = "JohnWiley & Sons, Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - Morphology, proliferation, and osteogenic differentiation of mesenchymal stem cells cultured on titanium, tantalum, and chromium surfaces

AU - Stiehler, Maik

AU - Lind, Martin

AU - Mygind, Tina

AU - Baatrup, Anette

AU - Dolatshahi-Pirouz, Alireza

AU - Li, Haisheng

AU - Foss, Morten

AU - Besenbacher, Flemming

AU - Kassem, Moustapha

AU - Bünger, Cody

PY - 2008

Y1 - 2008

N2 - Metallic implants are widely used in orthopedic surgery and dentistry. Durable osseous fixation of an implant requires that osteoprogenitor cells attach and adhere to the implant, proliferate, differentiate into osteoblasts, and produce mineralized matrix. In the present study, we investigated the interactions between human mesenchymal stem cells (MSCs) and smooth surfaces of titanium (Ti), tantalum (Ta), and chromium (Cr). Mean cellular area was quantified using fluorescence microscopy (4 h). Cellular proliferation was assessed by (3)H-thymidine incorporation and methylene blue cell counting assays (4 days). Osteogenic differentiation response was quantified by cell-specific alkaline phosphatase activity (ALP) assay (4 days), expression analysis of bone-related genes (4 days), and mineralization assay (21 days). Undifferentiated and osteogenically stimulated MSCs cultured on the different surfaces showed the same tendencies for proliferation and differentiation. MSCs exposed to Ti surfaces demonstrated enhanced proliferation compared with Ta and Cr surfaces. Cultivation of MSCs on Ta surfaces resulted in significantly increased mean cellular area and cell-specific ALP activity compared with the other surfaces tested. Cells cultured on Cr demonstrated reduced spreading and proliferation. In conclusion, Ta metal, as an alternative for Ti, can be considered as a promising biocompatible material, whereas further studies are needed to fully understand the role of Cr and its alloys in bone implants.

AB - Metallic implants are widely used in orthopedic surgery and dentistry. Durable osseous fixation of an implant requires that osteoprogenitor cells attach and adhere to the implant, proliferate, differentiate into osteoblasts, and produce mineralized matrix. In the present study, we investigated the interactions between human mesenchymal stem cells (MSCs) and smooth surfaces of titanium (Ti), tantalum (Ta), and chromium (Cr). Mean cellular area was quantified using fluorescence microscopy (4 h). Cellular proliferation was assessed by (3)H-thymidine incorporation and methylene blue cell counting assays (4 days). Osteogenic differentiation response was quantified by cell-specific alkaline phosphatase activity (ALP) assay (4 days), expression analysis of bone-related genes (4 days), and mineralization assay (21 days). Undifferentiated and osteogenically stimulated MSCs cultured on the different surfaces showed the same tendencies for proliferation and differentiation. MSCs exposed to Ti surfaces demonstrated enhanced proliferation compared with Ta and Cr surfaces. Cultivation of MSCs on Ta surfaces resulted in significantly increased mean cellular area and cell-specific ALP activity compared with the other surfaces tested. Cells cultured on Cr demonstrated reduced spreading and proliferation. In conclusion, Ta metal, as an alternative for Ti, can be considered as a promising biocompatible material, whereas further studies are needed to fully understand the role of Cr and its alloys in bone implants.

KW - Biocompatible Materials

KW - Cell Culture Techniques

KW - Cell Differentiation

KW - Cell Proliferation

KW - Cell Shape

KW - Chromium

KW - Dental Implants

KW - Humans

KW - Joint Prosthesis

KW - Mesenchymal Stem Cells

KW - Osteoblasts

KW - Osteogenesis

KW - Tantalum

KW - Titanium

U2 - 10.1002/jbm.a.31602

DO - 10.1002/jbm.a.31602

M3 - Journal article

C2 - 17975813

VL - 86

SP - 448

EP - 458

JO - Journal of Biomedical Materials Research. Part A

JF - Journal of Biomedical Materials Research. Part A

SN - 1549-3296

IS - 2

ER -