Molecular mechanism of sugar transport in plants unveiled by structures of glucose/H+ symporter STP10

Laust Bavnhøj, Peter Aasted Paulsen, Jose C. Flores-Canales, Birgit Schiøtt, Bjørn Panyella Pedersen*

*Corresponding author af dette arbejde

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Abstract

Sugars are essential sources of energy and carbon and also function as key signalling molecules in plants. Sugar transport proteins (STP) are proton-coupled symporters responsible for uptake of glucose from the apoplast into plant cells. They are integral to organ development in symplastically isolated tissues such as seed, pollen and fruit. Additionally, STPs play a vital role in plant responses to stressors such as dehydration and prevalent fungal infections like rust and mildew. Here we present a structure of Arabidopsis thaliana STP10 in the inward-open conformation at 2.6 Å resolution and a structure of the outward-occluded conformation at improved 1.8 Å resolution, both with glucose and protons bound. The two structures describe key states in the STP transport cycle. Together with molecular dynamics simulations that establish protonation states and biochemical analysis, they pinpoint structural elements, conserved in all STPs, that clarify the basis of proton-to-glucose coupling. These results advance our understanding of monosaccharide uptake, which is essential for plant organ development, and set the stage for bioengineering strategies in crops.

OriginalsprogEngelsk
TidsskriftNature Plants
Vol/bind7
Nummer10
Sider (fra-til)1409-1419
Antal sider11
ISSN2055-0278
DOI
StatusUdgivet - okt. 2021

Fingeraftryk

Dyk ned i forskningsemnerne om 'Molecular mechanism of sugar transport in plants unveiled by structures of glucose/H+ symporter STP10'. Sammen danner de et unikt fingeraftryk.

Citationsformater