Molecular epidemiology of ascariasis

TY - ABST

T1 - Molecular epidemiology of ascariasis

AU - Betson, Martha

AU - Halstead, Fennella

AU - Nejsum, Peter

AU - Olsen, Annette

AU - Stothard, Russel

PY - 2010

Y1 - 2010

N2 -   We are using molecular epidemiology techniques to study the population structure of Ascaris obtained from humans and pigs. Worms were obtained from human hosts on Zanzibar and in Uganda, Bangladesh, Guatemala and Nepal and Ascaris from pigs were collected from in Uganda, Tanzania, Denmark, Guatemala and the Philippines. Genomic DNA was extracted from each worm and a 450 base pair fragment of the mitochondrial cytochrome oxidase gene subunit 1 (COI) was PCR amplified. The products were sequenced from both strands and sequences were manually edited. Fifty different Ascaris CO1 haplotypes were identified in the 200+ worms included. Haplotypes 1 and 3 were common in Ascaris from human hosts and haplotypes 7, 33 and 36 was frequently found in worms obtained from pigs. Even though near complete segregation of Ascaris haplotypes between humans and pigs were seen, shared haplotypes were also observed suggesting retention of ancestral polymorphisme or cases of cross-infections. In addition, data suggests that speciation may have been driven not only by host affiliation but also by e.g. geography.

AB -   We are using molecular epidemiology techniques to study the population structure of Ascaris obtained from humans and pigs. Worms were obtained from human hosts on Zanzibar and in Uganda, Bangladesh, Guatemala and Nepal and Ascaris from pigs were collected from in Uganda, Tanzania, Denmark, Guatemala and the Philippines. Genomic DNA was extracted from each worm and a 450 base pair fragment of the mitochondrial cytochrome oxidase gene subunit 1 (COI) was PCR amplified. The products were sequenced from both strands and sequences were manually edited. Fifty different Ascaris CO1 haplotypes were identified in the 200+ worms included. Haplotypes 1 and 3 were common in Ascaris from human hosts and haplotypes 7, 33 and 36 was frequently found in worms obtained from pigs. Even though near complete segregation of Ascaris haplotypes between humans and pigs were seen, shared haplotypes were also observed suggesting retention of ancestral polymorphisme or cases of cross-infections. In addition, data suggests that speciation may have been driven not only by host affiliation but also by e.g. geography.

M3 - Conference abstract for conference

T2 - Joint Spring Symposium of the Danish Society for Parasitology & Danish Society for Tropical Medicine and International Health

Y2 - 30 April 2004 through 30 April 2004

ER -