Molecular cloning of the gene encoding developing seed l-asparaginase from Lupinus angustifolius

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  • J M J J Dickson, Danmark
  • Éva Vincze
  • M R Grant, Danmark
  • L A Smith, Danmark
  • K A Rodber, Danmark
  • K J F Farnden, Danmark
  • P H S Reynolds, Danmark
A genomic sequence encoding Lupinus angustifolius L-asparaginase has been obtained, and is the first report of this gene from a plant source. The 3.2 kb of DNA sequenced contains a 1136 bp 5' flanking sequence, four exons and three introns. Intron-exon borders were mapped by comparing the genomic sequence with that of a L. arboreus cDNA. Primer extension analysis revealed transcription start sites 16 bp and 13 bp 5' of the initiating ATG for L. angustifolius and L. arboreus, respectively. The 5' flanking region contained sequences associated with seed-specific expression.
OriginalsprogEngelsk
TidsskriftPlant Molecular Biology
Vol/bind20
Sider (fra-til)333-336
Antal sider4
ISSN0167-4412
StatusUdgivet - 1992
Eksternt udgivetJa

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