Molecular cloning and characterization of porcine Na⁺/K⁺-ATPase isoform α4

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Molecular cloning and characterization of porcine Na⁺/K⁺-ATPase isoform α4. / Larsen, Knud; Henriksen, Carina; Kristensen, Kaja Kjær; Momeni, Jamal; Farajzadeh, Leila.

I: Biochimie, Bind 158, 03.2019, s. 149-155.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Larsen, Knud ; Henriksen, Carina ; Kristensen, Kaja Kjær ; Momeni, Jamal ; Farajzadeh, Leila. / Molecular cloning and characterization of porcine Na⁺/K⁺-ATPase isoform α4. I: Biochimie. 2019 ; Bind 158. s. 149-155.

Bibtex

@article{a57057e9e1eb43109d8397854417ff41,
title = "Molecular cloning and characterization of porcine Na⁺/K⁺-ATPase isoform α4",
abstract = "Na+/K+-ATPase is responsible for maintaining electrochemical gradients of Na+ and K+, which is essential for a variety of cellular functions including neuronal activity. The α-subunit of the Na+/K+-ATPase is composed of four different polypeptides (α1-α4) encoded by different genes. Na,K-ATPase α4, encoded by the ATP1A4 gene, is expressed in testis and in male germ cells of humans, rats and mice. The α4 polypeptide has an important role in sperm motility, and is essential for male fertility. Here we present the RT-PCR cloning and characterization of the porcine ATP1A4 cDNA coding for Na⁺/K⁺-ATPase polypeptide α4. The Na⁺/K⁺-ATPase polypeptide α4, consisting of 1030 amino acids, displays a high homology with its human counterpart (86 {\%}). Phylogenetic analysis demonstrated that porcine Na⁺/K⁺-ATPase polypeptide α4 is closely related to other mammalian counterparts. In addition, the genomic structure of the porcine ATP1A4 gene was determined, and the intron-exon organization was found to be similar to that of the human ATP1A4 gene. The promoter sequence for the porcine ATP1A4 gene was also identified. Investigation of the genetic variation in the porcine ATP1A4 gene revealed a missense A/G SNP in exon 18. This A/G polymorphism results in a substitution of a methionine to a glycine residue (M888G). A very high overall DNA methylation rate of the ATP1A4 gene, 70-80 {\%}, was observed in both brain and liver. Expression analysis demonstrated that the porcine ATP1A4 gene is predominantly expressed in testis. The sequence of the porcine ATP1A4 cDNA encoding the Na⁺/K⁺-ATPase α4 protein has been submitted to GenBank under the accession number GenBank Accession No. MG587082.",
keywords = "ATP1A4, Methylation, Na⁺/K⁺-ATPase, Pig, SNP, Testis, Exons, Introns, Male, Mutation, Missense, Phylogeny, Sodium-Potassium-Exchanging ATPase/genetics, Animals, Isoenzymes/genetics, Swine, Cloning, Molecular, Polymorphism, Single Nucleotide, Amino Acid Substitution",
author = "Knud Larsen and Carina Henriksen and Kristensen, {Kaja Kj{\ae}r} and Jamal Momeni and Leila Farajzadeh",
note = "Copyright {\circledC} 2019 Elsevier B.V. and Soci{\'e}t{\'e} Fran{\cc}aise de Biochimie et Biologie Mol{\'e}culaire (SFBBM). All rights reserved.",
year = "2019",
month = "3",
doi = "10.1016/j.biochi.2019.01.003",
language = "English",
volume = "158",
pages = "149--155",
journal = "Biochimie",
issn = "0300-9084",
publisher = "Elsevier Masson",

}

RIS

TY - JOUR

T1 - Molecular cloning and characterization of porcine Na⁺/K⁺-ATPase isoform α4

AU - Larsen, Knud

AU - Henriksen, Carina

AU - Kristensen, Kaja Kjær

AU - Momeni, Jamal

AU - Farajzadeh, Leila

N1 - Copyright © 2019 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.

PY - 2019/3

Y1 - 2019/3

N2 - Na+/K+-ATPase is responsible for maintaining electrochemical gradients of Na+ and K+, which is essential for a variety of cellular functions including neuronal activity. The α-subunit of the Na+/K+-ATPase is composed of four different polypeptides (α1-α4) encoded by different genes. Na,K-ATPase α4, encoded by the ATP1A4 gene, is expressed in testis and in male germ cells of humans, rats and mice. The α4 polypeptide has an important role in sperm motility, and is essential for male fertility. Here we present the RT-PCR cloning and characterization of the porcine ATP1A4 cDNA coding for Na⁺/K⁺-ATPase polypeptide α4. The Na⁺/K⁺-ATPase polypeptide α4, consisting of 1030 amino acids, displays a high homology with its human counterpart (86 %). Phylogenetic analysis demonstrated that porcine Na⁺/K⁺-ATPase polypeptide α4 is closely related to other mammalian counterparts. In addition, the genomic structure of the porcine ATP1A4 gene was determined, and the intron-exon organization was found to be similar to that of the human ATP1A4 gene. The promoter sequence for the porcine ATP1A4 gene was also identified. Investigation of the genetic variation in the porcine ATP1A4 gene revealed a missense A/G SNP in exon 18. This A/G polymorphism results in a substitution of a methionine to a glycine residue (M888G). A very high overall DNA methylation rate of the ATP1A4 gene, 70-80 %, was observed in both brain and liver. Expression analysis demonstrated that the porcine ATP1A4 gene is predominantly expressed in testis. The sequence of the porcine ATP1A4 cDNA encoding the Na⁺/K⁺-ATPase α4 protein has been submitted to GenBank under the accession number GenBank Accession No. MG587082.

AB - Na+/K+-ATPase is responsible for maintaining electrochemical gradients of Na+ and K+, which is essential for a variety of cellular functions including neuronal activity. The α-subunit of the Na+/K+-ATPase is composed of four different polypeptides (α1-α4) encoded by different genes. Na,K-ATPase α4, encoded by the ATP1A4 gene, is expressed in testis and in male germ cells of humans, rats and mice. The α4 polypeptide has an important role in sperm motility, and is essential for male fertility. Here we present the RT-PCR cloning and characterization of the porcine ATP1A4 cDNA coding for Na⁺/K⁺-ATPase polypeptide α4. The Na⁺/K⁺-ATPase polypeptide α4, consisting of 1030 amino acids, displays a high homology with its human counterpart (86 %). Phylogenetic analysis demonstrated that porcine Na⁺/K⁺-ATPase polypeptide α4 is closely related to other mammalian counterparts. In addition, the genomic structure of the porcine ATP1A4 gene was determined, and the intron-exon organization was found to be similar to that of the human ATP1A4 gene. The promoter sequence for the porcine ATP1A4 gene was also identified. Investigation of the genetic variation in the porcine ATP1A4 gene revealed a missense A/G SNP in exon 18. This A/G polymorphism results in a substitution of a methionine to a glycine residue (M888G). A very high overall DNA methylation rate of the ATP1A4 gene, 70-80 %, was observed in both brain and liver. Expression analysis demonstrated that the porcine ATP1A4 gene is predominantly expressed in testis. The sequence of the porcine ATP1A4 cDNA encoding the Na⁺/K⁺-ATPase α4 protein has been submitted to GenBank under the accession number GenBank Accession No. MG587082.

KW - ATP1A4

KW - Methylation

KW - Na⁺/K⁺-ATPase

KW - Pig

KW - SNP

KW - Testis

KW - Exons

KW - Introns

KW - Male

KW - Mutation, Missense

KW - Phylogeny

KW - Sodium-Potassium-Exchanging ATPase/genetics

KW - Animals

KW - Isoenzymes/genetics

KW - Swine

KW - Cloning, Molecular

KW - Polymorphism, Single Nucleotide

KW - Amino Acid Substitution

U2 - 10.1016/j.biochi.2019.01.003

DO - 10.1016/j.biochi.2019.01.003

M3 - Journal article

VL - 158

SP - 149

EP - 155

JO - Biochimie

JF - Biochimie

SN - 0300-9084

ER -