Modular Assembly of Cell-targeting Devices Based on an Uncommon G-quadruplex Aptamer

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Standard

Modular Assembly of Cell-targeting Devices Based on an Uncommon G-quadruplex Aptamer. / Opazo, Felipe; Eiden, Laura; Hansen, Line; Rohrbach, Falk; Wengel, Jesper; Kjems, Jørgen; Mayer, Günter.

I: Molecular Therapy - Nucleic Acids, Bind 4, e251, 2015.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Opazo, F, Eiden, L, Hansen, L, Rohrbach, F, Wengel, J, Kjems, J & Mayer, G 2015, 'Modular Assembly of Cell-targeting Devices Based on an Uncommon G-quadruplex Aptamer', Molecular Therapy - Nucleic Acids, bind 4, e251. https://doi.org/10.1038/mtna.2015.25

APA

Opazo, F., Eiden, L., Hansen, L., Rohrbach, F., Wengel, J., Kjems, J., & Mayer, G. (2015). Modular Assembly of Cell-targeting Devices Based on an Uncommon G-quadruplex Aptamer. Molecular Therapy - Nucleic Acids, 4, [e251]. https://doi.org/10.1038/mtna.2015.25

CBE

Opazo F, Eiden L, Hansen L, Rohrbach F, Wengel J, Kjems J, Mayer G. 2015. Modular Assembly of Cell-targeting Devices Based on an Uncommon G-quadruplex Aptamer. Molecular Therapy - Nucleic Acids. 4:Article e251. https://doi.org/10.1038/mtna.2015.25

MLA

Vancouver

Opazo F, Eiden L, Hansen L, Rohrbach F, Wengel J, Kjems J o.a. Modular Assembly of Cell-targeting Devices Based on an Uncommon G-quadruplex Aptamer. Molecular Therapy - Nucleic Acids. 2015;4. e251. https://doi.org/10.1038/mtna.2015.25

Author

Opazo, Felipe ; Eiden, Laura ; Hansen, Line ; Rohrbach, Falk ; Wengel, Jesper ; Kjems, Jørgen ; Mayer, Günter. / Modular Assembly of Cell-targeting Devices Based on an Uncommon G-quadruplex Aptamer. I: Molecular Therapy - Nucleic Acids. 2015 ; Bind 4.

Bibtex

@article{6b93a80cfc634327b3eabc40d16bce2f,
title = "Modular Assembly of Cell-targeting Devices Based on an Uncommon G-quadruplex Aptamer",
abstract = "Aptamers are valuable tools that provide great potential to develop cost-effective diagnostics and therapies in the biomedical field. Here, we report a novel DNA aptamer that folds into an unconventional G-quadruplex structure able to recognize and enter specifically into human Burkitt's lymphoma cells. We further optimized this aptamer to a highly versatile and stable minimized version. The minimized aptamer can be easily equipped with different functionalities like quantum dots, organic dyes, or even a second different aptamer domain yielding a bi-paratopic aptamer. Although the target molecule of the aptamer remains unknown, our microscopy and pharmacological studies revealed that the aptamer hijacks the clathrin-mediated endocytosis pathway for its cellular internalization. We conclude that this novel class of aptamers can be used as a modular tool to specifically deliver different cargoes into malignant cells. This work provides a thorough characterization of the aptamer and we expect that our strategy will pave the path for future therapeutic applications.",
keywords = "LOCKED NUCLEIC-ACIDS, SIRNA CHIMERAS, CANCER-THERAPY, DRUG-DELIVERY, IN-VITRO, INHIBITOR, CHEMOTHERAPY, ENDOCYTOSIS, TRANSFERRIN, PROTEINS",
author = "Felipe Opazo and Laura Eiden and Line Hansen and Falk Rohrbach and Jesper Wengel and J{\o}rgen Kjems and G{\"u}nter Mayer",
year = "2015",
doi = "10.1038/mtna.2015.25",
language = "English",
volume = "4",
journal = "Molecular Therapy - Nucleic Acids",
issn = "2162-2531",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - Modular Assembly of Cell-targeting Devices Based on an Uncommon G-quadruplex Aptamer

AU - Opazo, Felipe

AU - Eiden, Laura

AU - Hansen, Line

AU - Rohrbach, Falk

AU - Wengel, Jesper

AU - Kjems, Jørgen

AU - Mayer, Günter

PY - 2015

Y1 - 2015

N2 - Aptamers are valuable tools that provide great potential to develop cost-effective diagnostics and therapies in the biomedical field. Here, we report a novel DNA aptamer that folds into an unconventional G-quadruplex structure able to recognize and enter specifically into human Burkitt's lymphoma cells. We further optimized this aptamer to a highly versatile and stable minimized version. The minimized aptamer can be easily equipped with different functionalities like quantum dots, organic dyes, or even a second different aptamer domain yielding a bi-paratopic aptamer. Although the target molecule of the aptamer remains unknown, our microscopy and pharmacological studies revealed that the aptamer hijacks the clathrin-mediated endocytosis pathway for its cellular internalization. We conclude that this novel class of aptamers can be used as a modular tool to specifically deliver different cargoes into malignant cells. This work provides a thorough characterization of the aptamer and we expect that our strategy will pave the path for future therapeutic applications.

AB - Aptamers are valuable tools that provide great potential to develop cost-effective diagnostics and therapies in the biomedical field. Here, we report a novel DNA aptamer that folds into an unconventional G-quadruplex structure able to recognize and enter specifically into human Burkitt's lymphoma cells. We further optimized this aptamer to a highly versatile and stable minimized version. The minimized aptamer can be easily equipped with different functionalities like quantum dots, organic dyes, or even a second different aptamer domain yielding a bi-paratopic aptamer. Although the target molecule of the aptamer remains unknown, our microscopy and pharmacological studies revealed that the aptamer hijacks the clathrin-mediated endocytosis pathway for its cellular internalization. We conclude that this novel class of aptamers can be used as a modular tool to specifically deliver different cargoes into malignant cells. This work provides a thorough characterization of the aptamer and we expect that our strategy will pave the path for future therapeutic applications.

KW - LOCKED NUCLEIC-ACIDS

KW - SIRNA CHIMERAS

KW - CANCER-THERAPY

KW - DRUG-DELIVERY

KW - IN-VITRO

KW - INHIBITOR

KW - CHEMOTHERAPY

KW - ENDOCYTOSIS

KW - TRANSFERRIN

KW - PROTEINS

U2 - 10.1038/mtna.2015.25

DO - 10.1038/mtna.2015.25

M3 - Journal article

C2 - 26325628

VL - 4

JO - Molecular Therapy - Nucleic Acids

JF - Molecular Therapy - Nucleic Acids

SN - 2162-2531

M1 - e251

ER -