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MIRRAGGE - Minimum Information Required for Reproducible AGGregation Experiments

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Review › Forskning › peer review

DOI

  • Pedro M Martins, University of Porto
    • ,
  • Susanna Navarro, Autonomous University of Barcelona
    • ,
  • Alexandra Silva, University of Porto
    • ,
  • Maria F Pinto, University of Porto
    • ,
  • Zsuzsa Sárkány, University of Porto
    • ,
  • Francisco Figueiredo, University of Porto, International Iberian Nanotechnology Laboratory
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  • Pedro José Barbosa Pereira, University of Porto
    • ,
  • Francisca Pinheiro, Autonomous University of Barcelona
    • ,
  • Zuzana Bednarikova, Slovak Academy of Sciences
    • ,
  • Michał Burdukiewicz, Faculty of Physics, Warsaw University of Technology, Warsaw, Poland.
    • ,
  • Oxana V Galzitskaya, Russian Acad Sci, Institute of Geography, Russian Academy of Sciences, Russian Academy of Sciences, Inst Geog
    • ,
  • Zuzana Gazova, Slovak Academy of Sciences
    • ,
  • Cláudio M Gomes, University of Lisbon
    • ,
  • Annalisa Pastore, UK-DRI Centre at King's College London
    • ,
  • Louise C Serpell, University of Sussex
    • ,
  • Rostislav Skrabana, Slovak Academy of Sciences, AXON Neuroscience CRM Services SE
    • ,
  • Vytautas Smirnovas, Vilnius University
    • ,
  • Mantas Ziaunys, Vilnius University
    • ,
  • Daniel E Otzen
  • Salvador Ventura, Autonomous University of Barcelona
    • ,
  • Sandra Macedo-Ribeiro, University of Porto

Reports on phase separation and amyloid formation for multiple proteins and aggregation-prone peptides are recurrently used to explore the molecular mechanisms associated with several human diseases. The information conveyed by these reports can be used directly in translational investigation, e.g., for the design of better drug screening strategies, or be compiled in databases for benchmarking novel aggregation-predicting algorithms. Given that minute protocol variations determine different outcomes of protein aggregation assays, there is a strong urge for standardized descriptions of the different types of aggregates and the detailed methods used in their production. In an attempt to address this need, we assembled the Minimum Information Required for Reproducible Aggregation Experiments (MIRRAGGE) guidelines, considering first-principles and the established literature on protein self-assembly and aggregation. This consensus information aims to cover the major and subtle determinants of experimental reproducibility while avoiding excessive technical details that are of limited practical interest for non-specialized users. The MIRRAGGE table (template available in Supplementary Information) is useful as a guide for the design of new studies and as a checklist during submission of experimental reports for publication. Full disclosure of relevant information also enables other researchers to reproduce results correctly and facilitates systematic data deposition into curated databases.

OriginalsprogEngelsk
Artikelnummer582488
TidsskriftFrontiers in Molecular Neuroscience
Vol/bind13
Antal sider18
ISSN1662-5099
DOI
StatusUdgivet - 27 nov. 2020

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