Minimal engraftment of human CD34+ cells mobilized from healthy donors in the infarcted heart of athymic nude rats

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Standard

Minimal engraftment of human CD34+ cells mobilized from healthy donors in the infarcted heart of athymic nude rats. / Sondergaard, Claus S; Bonde, Jesper; Dagnaes-Hansen, Frederik; Nielsen, Jan M; Zachar, Vladimir; Holm, Mette; Hokland, Peter; Pedersen, Lene Kirstine Eiby.

I: Stem Cells and Development, Bind 18, Nr. 6, 11.11.2008, s. 845-56.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Sondergaard, CS, Bonde, J, Dagnaes-Hansen, F, Nielsen, JM, Zachar, V, Holm, M, Hokland, P & Pedersen, LKE 2008, 'Minimal engraftment of human CD34+ cells mobilized from healthy donors in the infarcted heart of athymic nude rats', Stem Cells and Development, bind 18, nr. 6, s. 845-56. https://doi.org/10.1089/scd.2008.0006

APA

Sondergaard, C. S., Bonde, J., Dagnaes-Hansen, F., Nielsen, J. M., Zachar, V., Holm, M., Hokland, P., & Pedersen, L. K. E. (2008). Minimal engraftment of human CD34+ cells mobilized from healthy donors in the infarcted heart of athymic nude rats. Stem Cells and Development, 18(6), 845-56. https://doi.org/10.1089/scd.2008.0006

CBE

Sondergaard CS, Bonde J, Dagnaes-Hansen F, Nielsen JM, Zachar V, Holm M, Hokland P, Pedersen LKE. 2008. Minimal engraftment of human CD34+ cells mobilized from healthy donors in the infarcted heart of athymic nude rats. Stem Cells and Development. 18(6):845-56. https://doi.org/10.1089/scd.2008.0006

MLA

Vancouver

Sondergaard CS, Bonde J, Dagnaes-Hansen F, Nielsen JM, Zachar V, Holm M o.a. Minimal engraftment of human CD34+ cells mobilized from healthy donors in the infarcted heart of athymic nude rats. Stem Cells and Development. 2008 nov 11;18(6):845-56. https://doi.org/10.1089/scd.2008.0006

Author

Sondergaard, Claus S ; Bonde, Jesper ; Dagnaes-Hansen, Frederik ; Nielsen, Jan M ; Zachar, Vladimir ; Holm, Mette ; Hokland, Peter ; Pedersen, Lene Kirstine Eiby. / Minimal engraftment of human CD34+ cells mobilized from healthy donors in the infarcted heart of athymic nude rats. I: Stem Cells and Development. 2008 ; Bind 18, Nr. 6. s. 845-56.

Bibtex

@article{80970e05bc204e84ad825eea63e431c2,
title = "Minimal engraftment of human CD34+ cells mobilized from healthy donors in the infarcted heart of athymic nude rats",
abstract = "Cell-based regenerative therapy may be useful for treatment of acute myocardial infarction (AMI). Animal xenograft models are ideally suited for preclinical studies evaluating prospective treatment regimes, identifying candidate human cell populations, and gaining mechanistic insight. Here we address whether the athymic nude rat is suitable as a xenograft model for the study of human CD34+ mobilized peripheral blood stem cells (M-PBSCs) in the repair of AMI. We injected human donor cells into the infarct border of athymic nude rats with surgically induced AMI and evaluated engraftment and functional improvement. We found no human engraftment by immunofluorescence staining at 14 days after transplantation or functional improvement at days 2 and 14 compared to controls. The lack of long-term human engraftment was furthermore confirmed in a time series study analyzing animals at 0, 24, 48, 72, and 96 h after transplantation. Although we found fluorescent microbeads coinjected with human CD34+ M-PBSCs at all time points, the number of donor cells rapidly declined and became undetectable at 96 h. CD34+ M-PBSCs from the same donor used to treat athymic nude rat hearts engrafted the bone marrow of nonobese diabetic/severe combined immunodeficient mice 8-10 weeks after transplantation. In conclusion, human CD34+ M-PBSCs with confirmed hematopoietic engraftment potential rapidly disappeared from the site of injury following intramyocardial transplantation in the athymic nude rat AMI model.",
keywords = "Animals, Antigens, CD34, Antigens, CD45, Flow Cytometry, Fluorescence, Health, Hematopoiesis, Hematopoietic Stem Cell Mobilization, Hematopoietic Stem Cell Transplantation, Humans, Kinetics, Mice, Mice, SCID, Myocardial Infarction, Rats, Rats, Nude, Time Factors, Tissue Donors, Ventricular Function, Left",
author = "Sondergaard, {Claus S} and Jesper Bonde and Frederik Dagnaes-Hansen and Nielsen, {Jan M} and Vladimir Zachar and Mette Holm and Peter Hokland and Pedersen, {Lene Kirstine Eiby}",
year = "2008",
month = nov,
day = "11",
doi = "10.1089/scd.2008.0006",
language = "English",
volume = "18",
pages = "845--56",
journal = "Stem Cells and Development",
issn = "1547-3287",
publisher = "Mary AnnLiebert, Inc. Publishers",
number = "6",

}

RIS

TY - JOUR

T1 - Minimal engraftment of human CD34+ cells mobilized from healthy donors in the infarcted heart of athymic nude rats

AU - Sondergaard, Claus S

AU - Bonde, Jesper

AU - Dagnaes-Hansen, Frederik

AU - Nielsen, Jan M

AU - Zachar, Vladimir

AU - Holm, Mette

AU - Hokland, Peter

AU - Pedersen, Lene Kirstine Eiby

PY - 2008/11/11

Y1 - 2008/11/11

N2 - Cell-based regenerative therapy may be useful for treatment of acute myocardial infarction (AMI). Animal xenograft models are ideally suited for preclinical studies evaluating prospective treatment regimes, identifying candidate human cell populations, and gaining mechanistic insight. Here we address whether the athymic nude rat is suitable as a xenograft model for the study of human CD34+ mobilized peripheral blood stem cells (M-PBSCs) in the repair of AMI. We injected human donor cells into the infarct border of athymic nude rats with surgically induced AMI and evaluated engraftment and functional improvement. We found no human engraftment by immunofluorescence staining at 14 days after transplantation or functional improvement at days 2 and 14 compared to controls. The lack of long-term human engraftment was furthermore confirmed in a time series study analyzing animals at 0, 24, 48, 72, and 96 h after transplantation. Although we found fluorescent microbeads coinjected with human CD34+ M-PBSCs at all time points, the number of donor cells rapidly declined and became undetectable at 96 h. CD34+ M-PBSCs from the same donor used to treat athymic nude rat hearts engrafted the bone marrow of nonobese diabetic/severe combined immunodeficient mice 8-10 weeks after transplantation. In conclusion, human CD34+ M-PBSCs with confirmed hematopoietic engraftment potential rapidly disappeared from the site of injury following intramyocardial transplantation in the athymic nude rat AMI model.

AB - Cell-based regenerative therapy may be useful for treatment of acute myocardial infarction (AMI). Animal xenograft models are ideally suited for preclinical studies evaluating prospective treatment regimes, identifying candidate human cell populations, and gaining mechanistic insight. Here we address whether the athymic nude rat is suitable as a xenograft model for the study of human CD34+ mobilized peripheral blood stem cells (M-PBSCs) in the repair of AMI. We injected human donor cells into the infarct border of athymic nude rats with surgically induced AMI and evaluated engraftment and functional improvement. We found no human engraftment by immunofluorescence staining at 14 days after transplantation or functional improvement at days 2 and 14 compared to controls. The lack of long-term human engraftment was furthermore confirmed in a time series study analyzing animals at 0, 24, 48, 72, and 96 h after transplantation. Although we found fluorescent microbeads coinjected with human CD34+ M-PBSCs at all time points, the number of donor cells rapidly declined and became undetectable at 96 h. CD34+ M-PBSCs from the same donor used to treat athymic nude rat hearts engrafted the bone marrow of nonobese diabetic/severe combined immunodeficient mice 8-10 weeks after transplantation. In conclusion, human CD34+ M-PBSCs with confirmed hematopoietic engraftment potential rapidly disappeared from the site of injury following intramyocardial transplantation in the athymic nude rat AMI model.

KW - Animals

KW - Antigens, CD34

KW - Antigens, CD45

KW - Flow Cytometry

KW - Fluorescence

KW - Health

KW - Hematopoiesis

KW - Hematopoietic Stem Cell Mobilization

KW - Hematopoietic Stem Cell Transplantation

KW - Humans

KW - Kinetics

KW - Mice

KW - Mice, SCID

KW - Myocardial Infarction

KW - Rats

KW - Rats, Nude

KW - Time Factors

KW - Tissue Donors

KW - Ventricular Function, Left

U2 - 10.1089/scd.2008.0006

DO - 10.1089/scd.2008.0006

M3 - Journal article

C2 - 18991484

VL - 18

SP - 845

EP - 856

JO - Stem Cells and Development

JF - Stem Cells and Development

SN - 1547-3287

IS - 6

ER -