Mineralisation of soft and hard tissues and the stability of biofluids

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Standard

Mineralisation of soft and hard tissues and the stability of biofluids. / Holt, Carl; Lenton, Samuel; Nylander, Tommy; Sørensen, Esben Skipper; Teixeira, Susana C M.

I: Journal of Structural Biology, Bind 185, Nr. 3, 03.2014, s. 383-396.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Holt, C, Lenton, S, Nylander, T, Sørensen, ES & Teixeira, SCM 2014, 'Mineralisation of soft and hard tissues and the stability of biofluids', Journal of Structural Biology, bind 185, nr. 3, s. 383-396. https://doi.org/10.1016/j.jsb.2013.11.009

APA

Holt, C., Lenton, S., Nylander, T., Sørensen, E. S., & Teixeira, S. C. M. (2014). Mineralisation of soft and hard tissues and the stability of biofluids. Journal of Structural Biology, 185(3), 383-396. https://doi.org/10.1016/j.jsb.2013.11.009

CBE

Holt C, Lenton S, Nylander T, Sørensen ES, Teixeira SCM. 2014. Mineralisation of soft and hard tissues and the stability of biofluids. Journal of Structural Biology. 185(3):383-396. https://doi.org/10.1016/j.jsb.2013.11.009

MLA

Vancouver

Holt C, Lenton S, Nylander T, Sørensen ES, Teixeira SCM. Mineralisation of soft and hard tissues and the stability of biofluids. Journal of Structural Biology. 2014 mar;185(3):383-396. https://doi.org/10.1016/j.jsb.2013.11.009

Author

Holt, Carl ; Lenton, Samuel ; Nylander, Tommy ; Sørensen, Esben Skipper ; Teixeira, Susana C M. / Mineralisation of soft and hard tissues and the stability of biofluids. I: Journal of Structural Biology. 2014 ; Bind 185, Nr. 3. s. 383-396.

Bibtex

@article{421b187d63934fe1a831a1ccbd1808df,
title = "Mineralisation of soft and hard tissues and the stability of biofluids",
abstract = "Evidence is provided from studies on natural and artificial biofluids that the sequestration of amorphous calcium phosphate by peptides or proteins to form nanocluster complexes is of general importance in the control of physiological calcification. A naturally occurring mixture of osteopontin peptides was shown, by light and neutron scattering, to form calcium phosphate nanoclusters with a core-shell structure. In blood serum and stimulated saliva, an invariant calcium phosphate ion activity product was found which corresponds closely in form and magnitude to the ion activity product observed in solutions of these osteopontin nanoclusters. This suggests that types of nanocluster complexes are present in these biofluids as well as in milk. Precipitation of amorphous calcium phosphate from artificial blood serum, urine and saliva was determined as a function of pH and the concentration of osteopontin or casein phosphopeptides. The position of the boundary between stability and precipitation was found to agree quantitatively with the theory of nanocluster formation. Artificial biofluids were prepared that closely matched their natural counterparts in calcium and phosphate concentrations, pH, saturation, ionic strength and osmolality. Such fluids, stabilised by a low concentration of sequestering phosphopeptides, were found to be highly stable and may have a number of beneficial applications in medicine.",
author = "Carl Holt and Samuel Lenton and Tommy Nylander and S{\o}rensen, {Esben Skipper} and Teixeira, {Susana C M}",
note = "Copyright {\textcopyright} 2013 The Authors. Published by Elsevier Inc. All rights reserved.",
year = "2014",
month = mar,
doi = "10.1016/j.jsb.2013.11.009",
language = "English",
volume = "185",
pages = "383--396",
journal = "Journal of Structural Biology",
issn = "1047-8477",
publisher = "Academic Press",
number = "3",

}

RIS

TY - JOUR

T1 - Mineralisation of soft and hard tissues and the stability of biofluids

AU - Holt, Carl

AU - Lenton, Samuel

AU - Nylander, Tommy

AU - Sørensen, Esben Skipper

AU - Teixeira, Susana C M

N1 - Copyright © 2013 The Authors. Published by Elsevier Inc. All rights reserved.

PY - 2014/3

Y1 - 2014/3

N2 - Evidence is provided from studies on natural and artificial biofluids that the sequestration of amorphous calcium phosphate by peptides or proteins to form nanocluster complexes is of general importance in the control of physiological calcification. A naturally occurring mixture of osteopontin peptides was shown, by light and neutron scattering, to form calcium phosphate nanoclusters with a core-shell structure. In blood serum and stimulated saliva, an invariant calcium phosphate ion activity product was found which corresponds closely in form and magnitude to the ion activity product observed in solutions of these osteopontin nanoclusters. This suggests that types of nanocluster complexes are present in these biofluids as well as in milk. Precipitation of amorphous calcium phosphate from artificial blood serum, urine and saliva was determined as a function of pH and the concentration of osteopontin or casein phosphopeptides. The position of the boundary between stability and precipitation was found to agree quantitatively with the theory of nanocluster formation. Artificial biofluids were prepared that closely matched their natural counterparts in calcium and phosphate concentrations, pH, saturation, ionic strength and osmolality. Such fluids, stabilised by a low concentration of sequestering phosphopeptides, were found to be highly stable and may have a number of beneficial applications in medicine.

AB - Evidence is provided from studies on natural and artificial biofluids that the sequestration of amorphous calcium phosphate by peptides or proteins to form nanocluster complexes is of general importance in the control of physiological calcification. A naturally occurring mixture of osteopontin peptides was shown, by light and neutron scattering, to form calcium phosphate nanoclusters with a core-shell structure. In blood serum and stimulated saliva, an invariant calcium phosphate ion activity product was found which corresponds closely in form and magnitude to the ion activity product observed in solutions of these osteopontin nanoclusters. This suggests that types of nanocluster complexes are present in these biofluids as well as in milk. Precipitation of amorphous calcium phosphate from artificial blood serum, urine and saliva was determined as a function of pH and the concentration of osteopontin or casein phosphopeptides. The position of the boundary between stability and precipitation was found to agree quantitatively with the theory of nanocluster formation. Artificial biofluids were prepared that closely matched their natural counterparts in calcium and phosphate concentrations, pH, saturation, ionic strength and osmolality. Such fluids, stabilised by a low concentration of sequestering phosphopeptides, were found to be highly stable and may have a number of beneficial applications in medicine.

U2 - 10.1016/j.jsb.2013.11.009

DO - 10.1016/j.jsb.2013.11.009

M3 - Journal article

C2 - 24316224

VL - 185

SP - 383

EP - 396

JO - Journal of Structural Biology

JF - Journal of Structural Biology

SN - 1047-8477

IS - 3

ER -