Aarhus University Seal / Aarhus Universitets segl

Mind the gap (junction): cGMP induced by nitric oxide in cardiac myocytes originates from cardiac fibroblasts

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Standard

Mind the gap (junction) : cGMP induced by nitric oxide in cardiac myocytes originates from cardiac fibroblasts. / Menges, Lukas; Krawutschke, Christian; Füchtbauer, Ernst Martin; Füchtbauer, Annette; Sandner, Peter; Koesling, Doris; Russwurm, Michael.

I: British Journal of Pharmacology, Bind 176, Nr. 24, 01.12.2019, s. 4696-4707.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Menges, L, Krawutschke, C, Füchtbauer, EM, Füchtbauer, A, Sandner, P, Koesling, D & Russwurm, M 2019, 'Mind the gap (junction): cGMP induced by nitric oxide in cardiac myocytes originates from cardiac fibroblasts', British Journal of Pharmacology, bind 176, nr. 24, s. 4696-4707. https://doi.org/10.1111/bph.14835

APA

Menges, L., Krawutschke, C., Füchtbauer, E. M., Füchtbauer, A., Sandner, P., Koesling, D., & Russwurm, M. (2019). Mind the gap (junction): cGMP induced by nitric oxide in cardiac myocytes originates from cardiac fibroblasts. British Journal of Pharmacology, 176(24), 4696-4707. https://doi.org/10.1111/bph.14835

CBE

Menges L, Krawutschke C, Füchtbauer EM, Füchtbauer A, Sandner P, Koesling D, Russwurm M. 2019. Mind the gap (junction): cGMP induced by nitric oxide in cardiac myocytes originates from cardiac fibroblasts. British Journal of Pharmacology. 176(24):4696-4707. https://doi.org/10.1111/bph.14835

MLA

Vancouver

Menges L, Krawutschke C, Füchtbauer EM, Füchtbauer A, Sandner P, Koesling D o.a. Mind the gap (junction): cGMP induced by nitric oxide in cardiac myocytes originates from cardiac fibroblasts. British Journal of Pharmacology. 2019 dec 1;176(24):4696-4707. https://doi.org/10.1111/bph.14835

Author

Menges, Lukas ; Krawutschke, Christian ; Füchtbauer, Ernst Martin ; Füchtbauer, Annette ; Sandner, Peter ; Koesling, Doris ; Russwurm, Michael. / Mind the gap (junction) : cGMP induced by nitric oxide in cardiac myocytes originates from cardiac fibroblasts. I: British Journal of Pharmacology. 2019 ; Bind 176, Nr. 24. s. 4696-4707.

Bibtex

@article{ab6f9b14abc4446eae61f2e7ca1c097d,
title = "Mind the gap (junction): cGMP induced by nitric oxide in cardiac myocytes originates from cardiac fibroblasts",
abstract = "Background and Purpose: The intracellular signalling molecule cGMP, formed by NO-sensitive GC (NO–GC), has an established function in the vascular system. Despite numerous reports about NO-induced cGMP effects in the heart, the underlying cGMP signals are poorly characterized. Experimental Approach: Therefore, we analysed cGMP signals in cardiac myocytes and fibroblasts isolated from knock-in mice expressing a FRET-based cGMP indicator. Key Results: Whereas in cardiac myocytes, none of the known NO–GC-activating substances (NO, GC activators, and GC stimulators) increased cGMP even in the presence of PDE inhibitors, they induced substantial cGMP increases in cardiac fibroblasts. As cardiac myocytes and fibroblasts are electrically connected via gap junctions, we asked whether cGMP can take the same route. Indeed, in cardiomyocytes co-cultured on cardiac fibroblasts, NO-induced cGMP signals were detectable, and two groups of unrelated gap junction inhibitors abolished these signals. Conclusion and Implication: We conclude that NO-induced cGMP formed in cardiac fibroblasts enters cardiac myocytes via gap junctions thereby turning cGMP into an intercellular signalling molecule. The findings shed new light on NO/cGMP signalling in the heart and will potentially broaden therapeutic opportunities for cardiac disease.",
author = "Lukas Menges and Christian Krawutschke and F{\"u}chtbauer, {Ernst Martin} and Annette F{\"u}chtbauer and Peter Sandner and Doris Koesling and Michael Russwurm",
note = "Publisher Copyright: {\textcopyright} 2019 The Authors. British Journal of Pharmacology published by John Wiley & Sons Ltd on behalf of British Pharmacological Society Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",
year = "2019",
month = dec,
day = "1",
doi = "10.1111/bph.14835",
language = "English",
volume = "176",
pages = "4696--4707",
journal = "British Journal of Pharmacology",
issn = "0007-1188",
publisher = "John/Wiley & Sons Ltd.",
number = "24",

}

RIS

TY - JOUR

T1 - Mind the gap (junction)

T2 - cGMP induced by nitric oxide in cardiac myocytes originates from cardiac fibroblasts

AU - Menges, Lukas

AU - Krawutschke, Christian

AU - Füchtbauer, Ernst Martin

AU - Füchtbauer, Annette

AU - Sandner, Peter

AU - Koesling, Doris

AU - Russwurm, Michael

N1 - Publisher Copyright: © 2019 The Authors. British Journal of Pharmacology published by John Wiley & Sons Ltd on behalf of British Pharmacological Society Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2019/12/1

Y1 - 2019/12/1

N2 - Background and Purpose: The intracellular signalling molecule cGMP, formed by NO-sensitive GC (NO–GC), has an established function in the vascular system. Despite numerous reports about NO-induced cGMP effects in the heart, the underlying cGMP signals are poorly characterized. Experimental Approach: Therefore, we analysed cGMP signals in cardiac myocytes and fibroblasts isolated from knock-in mice expressing a FRET-based cGMP indicator. Key Results: Whereas in cardiac myocytes, none of the known NO–GC-activating substances (NO, GC activators, and GC stimulators) increased cGMP even in the presence of PDE inhibitors, they induced substantial cGMP increases in cardiac fibroblasts. As cardiac myocytes and fibroblasts are electrically connected via gap junctions, we asked whether cGMP can take the same route. Indeed, in cardiomyocytes co-cultured on cardiac fibroblasts, NO-induced cGMP signals were detectable, and two groups of unrelated gap junction inhibitors abolished these signals. Conclusion and Implication: We conclude that NO-induced cGMP formed in cardiac fibroblasts enters cardiac myocytes via gap junctions thereby turning cGMP into an intercellular signalling molecule. The findings shed new light on NO/cGMP signalling in the heart and will potentially broaden therapeutic opportunities for cardiac disease.

AB - Background and Purpose: The intracellular signalling molecule cGMP, formed by NO-sensitive GC (NO–GC), has an established function in the vascular system. Despite numerous reports about NO-induced cGMP effects in the heart, the underlying cGMP signals are poorly characterized. Experimental Approach: Therefore, we analysed cGMP signals in cardiac myocytes and fibroblasts isolated from knock-in mice expressing a FRET-based cGMP indicator. Key Results: Whereas in cardiac myocytes, none of the known NO–GC-activating substances (NO, GC activators, and GC stimulators) increased cGMP even in the presence of PDE inhibitors, they induced substantial cGMP increases in cardiac fibroblasts. As cardiac myocytes and fibroblasts are electrically connected via gap junctions, we asked whether cGMP can take the same route. Indeed, in cardiomyocytes co-cultured on cardiac fibroblasts, NO-induced cGMP signals were detectable, and two groups of unrelated gap junction inhibitors abolished these signals. Conclusion and Implication: We conclude that NO-induced cGMP formed in cardiac fibroblasts enters cardiac myocytes via gap junctions thereby turning cGMP into an intercellular signalling molecule. The findings shed new light on NO/cGMP signalling in the heart and will potentially broaden therapeutic opportunities for cardiac disease.

UR - http://www.scopus.com/inward/record.url?scp=85077383843&partnerID=8YFLogxK

U2 - 10.1111/bph.14835

DO - 10.1111/bph.14835

M3 - Journal article

C2 - 31423565

AN - SCOPUS:85077383843

VL - 176

SP - 4696

EP - 4707

JO - British Journal of Pharmacology

JF - British Journal of Pharmacology

SN - 0007-1188

IS - 24

ER -