Microselection - Affinity Selecting Antibodies against a Single Rare Cell in a Heterogeneous Population

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Standard

Microselection - Affinity Selecting Antibodies against a Single Rare Cell in a Heterogeneous Population. / Sørensen, Morten Draeby; Agerholm, Inge Errebo; Christensen, Britta; Kølvraa, Steen; Kristensen, Peter.

I: Journal of Cellular and Molecular Medicine, Bind 14, Nr. 7, 01.07.2010, s. 1953-1961.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

Harvard

Sørensen, MD, Agerholm, IE, Christensen, B, Kølvraa, S & Kristensen, P 2010, 'Microselection - Affinity Selecting Antibodies against a Single Rare Cell in a Heterogeneous Population', Journal of Cellular and Molecular Medicine, bind 14, nr. 7, s. 1953-1961. https://doi.org/10.1111/j.1582-4934.2009.00896.x

APA

Sørensen, M. D., Agerholm, I. E., Christensen, B., Kølvraa, S., & Kristensen, P. (2010). Microselection - Affinity Selecting Antibodies against a Single Rare Cell in a Heterogeneous Population. Journal of Cellular and Molecular Medicine, 14(7), 1953-1961. https://doi.org/10.1111/j.1582-4934.2009.00896.x

CBE

Sørensen MD, Agerholm IE, Christensen B, Kølvraa S, Kristensen P. 2010. Microselection - Affinity Selecting Antibodies against a Single Rare Cell in a Heterogeneous Population. Journal of Cellular and Molecular Medicine. 14(7):1953-1961. https://doi.org/10.1111/j.1582-4934.2009.00896.x

MLA

Vancouver

Sørensen MD, Agerholm IE, Christensen B, Kølvraa S, Kristensen P. Microselection - Affinity Selecting Antibodies against a Single Rare Cell in a Heterogeneous Population. Journal of Cellular and Molecular Medicine. 2010 jul 1;14(7):1953-1961. https://doi.org/10.1111/j.1582-4934.2009.00896.x

Author

Sørensen, Morten Draeby ; Agerholm, Inge Errebo ; Christensen, Britta ; Kølvraa, Steen ; Kristensen, Peter. / Microselection - Affinity Selecting Antibodies against a Single Rare Cell in a Heterogeneous Population. I: Journal of Cellular and Molecular Medicine. 2010 ; Bind 14, Nr. 7. s. 1953-1961.

Bibtex

@article{68b158e0b86e11de82fe000ea68e967b,
title = "Microselection - Affinity Selecting Antibodies against a Single Rare Cell in a Heterogeneous Population",
abstract = "Rare cells not normally present in the peripheral bloodstream, such as circulating tumour cells, have potential applications for development of non-invasive methods for diagnostics or follow up. Obtaining these cells however require some means of discrimination, achievable by cell type specific antibodies. Here we have generated a microselection method allowing antibody selection, by phage display, targeting a single cell in a heterogeneous population. One K562 cell (female origin) was positioned on glass slide among millions of lymphocytes from male donor, identifying the K562 cell by FISH (XX). Several single cell selections were performed on such individual slides. The phage particles bound to the target cell is protected by a minute disc, while inactivating all remaining phage by UV-irradiation; leaving only the phage bound to the target cell viable. We hereby retrieved up to eight antibodies per single cell selection, including three highly K562 cell type specific.",
author = "S{\o}rensen, {Morten Draeby} and Agerholm, {Inge Errebo} and Britta Christensen and Steen K{\o}lvraa and Peter Kristensen",
note = "Paper id:: DOI: 10.1111/j.1582-4934.2010.00896.x",
year = "2010",
month = "7",
day = "1",
doi = "10.1111/j.1582-4934.2009.00896.x",
language = "English",
volume = "14",
pages = "1953--1961",
journal = "Journal of Cellular and Molecular Medicine",
issn = "1582-1838",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "7",

}

RIS

TY - JOUR

T1 - Microselection - Affinity Selecting Antibodies against a Single Rare Cell in a Heterogeneous Population

AU - Sørensen, Morten Draeby

AU - Agerholm, Inge Errebo

AU - Christensen, Britta

AU - Kølvraa, Steen

AU - Kristensen, Peter

N1 - Paper id:: DOI: 10.1111/j.1582-4934.2010.00896.x

PY - 2010/7/1

Y1 - 2010/7/1

N2 - Rare cells not normally present in the peripheral bloodstream, such as circulating tumour cells, have potential applications for development of non-invasive methods for diagnostics or follow up. Obtaining these cells however require some means of discrimination, achievable by cell type specific antibodies. Here we have generated a microselection method allowing antibody selection, by phage display, targeting a single cell in a heterogeneous population. One K562 cell (female origin) was positioned on glass slide among millions of lymphocytes from male donor, identifying the K562 cell by FISH (XX). Several single cell selections were performed on such individual slides. The phage particles bound to the target cell is protected by a minute disc, while inactivating all remaining phage by UV-irradiation; leaving only the phage bound to the target cell viable. We hereby retrieved up to eight antibodies per single cell selection, including three highly K562 cell type specific.

AB - Rare cells not normally present in the peripheral bloodstream, such as circulating tumour cells, have potential applications for development of non-invasive methods for diagnostics or follow up. Obtaining these cells however require some means of discrimination, achievable by cell type specific antibodies. Here we have generated a microselection method allowing antibody selection, by phage display, targeting a single cell in a heterogeneous population. One K562 cell (female origin) was positioned on glass slide among millions of lymphocytes from male donor, identifying the K562 cell by FISH (XX). Several single cell selections were performed on such individual slides. The phage particles bound to the target cell is protected by a minute disc, while inactivating all remaining phage by UV-irradiation; leaving only the phage bound to the target cell viable. We hereby retrieved up to eight antibodies per single cell selection, including three highly K562 cell type specific.

U2 - 10.1111/j.1582-4934.2009.00896.x

DO - 10.1111/j.1582-4934.2009.00896.x

M3 - Journal article

C2 - 19754674

VL - 14

SP - 1953

EP - 1961

JO - Journal of Cellular and Molecular Medicine

JF - Journal of Cellular and Molecular Medicine

SN - 1582-1838

IS - 7

ER -