MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides

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MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides. / Lindahl, Lise M; Gluud, Maria; Emmanuel, Thomas; Thomsen, Emil A; Hu, Tengpeng; Rittig, Anne H; Celis, Pamela; Stolearenco, Veronica; Krejsgaard, Thorbjørn; Johansen, Claus; Willerslev-Olsen, Andreas; Buus, Terkild B; Woetmann, Anders; Aagaard, Lars; Geisler, Carsten; Litman, Thomas; Mikkelsen, Jacob G; Odum, Niels; Iversen, Lars.

I: Acta Dermato-Venereologica, Bind 100, adv00270, 09.2020.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avisTidsskriftartikelForskningpeer review

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Lindahl, Lise M ; Gluud, Maria ; Emmanuel, Thomas ; Thomsen, Emil A ; Hu, Tengpeng ; Rittig, Anne H ; Celis, Pamela ; Stolearenco, Veronica ; Krejsgaard, Thorbjørn ; Johansen, Claus ; Willerslev-Olsen, Andreas ; Buus, Terkild B ; Woetmann, Anders ; Aagaard, Lars ; Geisler, Carsten ; Litman, Thomas ; Mikkelsen, Jacob G ; Odum, Niels ; Iversen, Lars. / MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides. I: Acta Dermato-Venereologica. 2020 ; Bind 100.

Bibtex

@article{a4a34454cdb5453fa50bee456d32b312,
title = "MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides",
abstract = "A prognostic 3-miRNA classifier for early-stage mycosis fungoides has been developed recently, with miR-106b providing the strongest prognostic power. The aim of this study was to investigate the molecular function of miR-106b in mycosis fungoides disease progression. The cellular localization of miR-106b in mycosis fungoides skin biopsies was determined by in situ hybridization. The regulatory role of miR-106b was assessed by transient miR-106b inhibitor/mimic transfection of 2 mycosis fungoides derived cell lines, followed by quantitative real-time PCR (RT-qPCR), western blotting and a proliferation assay. MiR-106b was found to be expressed by dermal T-lymphocytes in mycosis fungoides skin lesions, and miR-106b expression increased with advancing mycosis fungoides stage. Transfection of miR-106b in 2 mycosis fungoides derived cell lines showed that miR-106b represses the tumour suppressors cyclin-dependent kinase inhibitor 1 (p21) and thioredoxin-interacting protein (TXNIP) and promotes mycosis fungoides tumour cell proliferation. In conclusion, these results substantiate that miR-106b has both a functional and prognostic role in progression of mycosis fungoides.",
author = "Lindahl, {Lise M} and Maria Gluud and Thomas Emmanuel and Thomsen, {Emil A} and Tengpeng Hu and Rittig, {Anne H} and Pamela Celis and Veronica Stolearenco and Thorbj{\o}rn Krejsgaard and Claus Johansen and Andreas Willerslev-Olsen and Buus, {Terkild B} and Anders Woetmann and Lars Aagaard and Carsten Geisler and Thomas Litman and Mikkelsen, {Jacob G} and Niels Odum and Lars Iversen",
year = "2020",
month = sep,
doi = "10.2340/00015555-3574",
language = "English",
volume = "100",
journal = "Acta Dermatovenereologica",
issn = "0001-5555",
publisher = "Society for the Publication of Acta Dermato - Venereologica",

}

RIS

TY - JOUR

T1 - MicroRNA-106b Regulates Expression of the Tumour Suppressors p21 and TXNIP and Promotes Tumour Cell Proliferation in Mycosis Fungoides

AU - Lindahl, Lise M

AU - Gluud, Maria

AU - Emmanuel, Thomas

AU - Thomsen, Emil A

AU - Hu, Tengpeng

AU - Rittig, Anne H

AU - Celis, Pamela

AU - Stolearenco, Veronica

AU - Krejsgaard, Thorbjørn

AU - Johansen, Claus

AU - Willerslev-Olsen, Andreas

AU - Buus, Terkild B

AU - Woetmann, Anders

AU - Aagaard, Lars

AU - Geisler, Carsten

AU - Litman, Thomas

AU - Mikkelsen, Jacob G

AU - Odum, Niels

AU - Iversen, Lars

PY - 2020/9

Y1 - 2020/9

N2 - A prognostic 3-miRNA classifier for early-stage mycosis fungoides has been developed recently, with miR-106b providing the strongest prognostic power. The aim of this study was to investigate the molecular function of miR-106b in mycosis fungoides disease progression. The cellular localization of miR-106b in mycosis fungoides skin biopsies was determined by in situ hybridization. The regulatory role of miR-106b was assessed by transient miR-106b inhibitor/mimic transfection of 2 mycosis fungoides derived cell lines, followed by quantitative real-time PCR (RT-qPCR), western blotting and a proliferation assay. MiR-106b was found to be expressed by dermal T-lymphocytes in mycosis fungoides skin lesions, and miR-106b expression increased with advancing mycosis fungoides stage. Transfection of miR-106b in 2 mycosis fungoides derived cell lines showed that miR-106b represses the tumour suppressors cyclin-dependent kinase inhibitor 1 (p21) and thioredoxin-interacting protein (TXNIP) and promotes mycosis fungoides tumour cell proliferation. In conclusion, these results substantiate that miR-106b has both a functional and prognostic role in progression of mycosis fungoides.

AB - A prognostic 3-miRNA classifier for early-stage mycosis fungoides has been developed recently, with miR-106b providing the strongest prognostic power. The aim of this study was to investigate the molecular function of miR-106b in mycosis fungoides disease progression. The cellular localization of miR-106b in mycosis fungoides skin biopsies was determined by in situ hybridization. The regulatory role of miR-106b was assessed by transient miR-106b inhibitor/mimic transfection of 2 mycosis fungoides derived cell lines, followed by quantitative real-time PCR (RT-qPCR), western blotting and a proliferation assay. MiR-106b was found to be expressed by dermal T-lymphocytes in mycosis fungoides skin lesions, and miR-106b expression increased with advancing mycosis fungoides stage. Transfection of miR-106b in 2 mycosis fungoides derived cell lines showed that miR-106b represses the tumour suppressors cyclin-dependent kinase inhibitor 1 (p21) and thioredoxin-interacting protein (TXNIP) and promotes mycosis fungoides tumour cell proliferation. In conclusion, these results substantiate that miR-106b has both a functional and prognostic role in progression of mycosis fungoides.

U2 - 10.2340/00015555-3574

DO - 10.2340/00015555-3574

M3 - Journal article

C2 - 32556351

VL - 100

JO - Acta Dermatovenereologica

JF - Acta Dermatovenereologica

SN - 0001-5555

M1 - adv00270

ER -