Microfluidics-mediated isothermal detection of enzyme activity at the single molecule level

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Conventional analysis of enzymatic activity, often carried out on pools of cells, is blind to heterogeneity in the population. Here, we combine microfluidics with a previously developed isothermal rolling circle amplification-based assay to investigate multiple enzymatic activities in down to single cells. This microfluidics-meditated assay performs at very high sensitivity in picoliter incubators with small quantities of biological materials. Furthermore, we demonstrate the assay's capability of multiplexed detection of at least three enzyme activities at the single molecule level.
OriginalsprogEngelsk
TidsskriftI E E E Engineering in Medicine and Biology Society. Conference Proceedings
Vol/bind2011
Sider (fra-til)3258-3261
Antal sider4
ISSN2375-7477
DOI
StatusUdgivet - 2011
Begivenhed2011 Annual International Conference of the IEEE - Boston, USA
Varighed: 30 aug. 20113 sep. 2011

Konference

Konference2011 Annual International Conference of the IEEE
LandUSA
ByBoston
Periode30/08/201103/09/2011

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