Microfluidics-mediated isothermal detection of enzyme activity at the single molecule level

Sissel Juul; Yi-Ping Ho; Magnus Stougaard; Jørn Koch; Felicie F Andersen; Kam W Leong; Birgitta R Knudsen

doi:10.1109/IEMBS.2011.6090885

Microfluidics-mediated isothermal detection of enzyme activity at the single molecule level

Sissel Juul, Yi-Ping Ho, Magnus Stougaard, Jørn Koch, Felicie F Andersen, Kam W Leong, Birgitta R Knudsen

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Konferenceartikel › Forskning › peer review

4 Citationer (Scopus)

Abstract

Conventional analysis of enzymatic activity, often carried out on pools of cells, is blind to heterogeneity in the population. Here, we combine microfluidics with a previously developed isothermal rolling circle amplification-based assay to investigate multiple enzymatic activities in down to single cells. This microfluidics-meditated assay performs at very high sensitivity in picoliter incubators with small quantities of biological materials. Furthermore, we demonstrate the assay's capability of multiplexed detection of at least three enzyme activities at the single molecule level.

Originalsprog	Engelsk
Tidsskrift	I E E E Engineering in Medicine and Biology Society. Conference Proceedings
Vol/bind	2011
Sider (fra-til)	3258-3261
Antal sider	4
ISSN	2375-7477
DOI	https://doi.org/10.1109/IEMBS.2011.6090885
Status	Udgivet - 2011
Begivenhed	2011 Annual International Conference of the IEEE - Boston, USA Varighed: 30 aug. 2011 → 3 sep. 2011

Konference

Konference	2011 Annual International Conference of the IEEE
Land/Område	USA
By	Boston
Periode	30/08/2011 → 03/09/2011

Adgang til dokumentet

10.1109/IEMBS.2011.6090885

Tjek tilgængelighed hos det Kgl. Bibliotek

Citationsformater

@inproceedings{cdb8b73995254275a045e087c1a9e522,

title = "Microfluidics-mediated isothermal detection of enzyme activity at the single molecule level",

abstract = "Conventional analysis of enzymatic activity, often carried out on pools of cells, is blind to heterogeneity in the population. Here, we combine microfluidics with a previously developed isothermal rolling circle amplification-based assay to investigate multiple enzymatic activities in down to single cells. This microfluidics-meditated assay performs at very high sensitivity in picoliter incubators with small quantities of biological materials. Furthermore, we demonstrate the assay's capability of multiplexed detection of at least three enzyme activities at the single molecule level.",

keywords = "Cell Line, Enzymes, Humans, Microfluidics",

author = "Sissel Juul and Yi-Ping Ho and Magnus Stougaard and J{\o}rn Koch and Andersen, {Felicie F} and Leong, {Kam W} and Knudsen, {Birgitta R}",

year = "2011",

doi = "10.1109/IEMBS.2011.6090885",

language = "English",

volume = "2011",

pages = "3258--3261",

journal = "I E E E Engineering in Medicine and Biology Society. Conference Proceedings",

issn = "2375-7477",

publisher = "IEEE ",

note = "2011 Annual International Conference of the IEEE ; Conference date: 30-08-2011 Through 03-09-2011",

}

Microfluidics-mediated isothermal detection of enzyme activity at the single molecule level. / Juul, Sissel; Ho, Yi-Ping; Stougaard, Magnus et al.
I: I E E E Engineering in Medicine and Biology Society. Conference Proceedings, Bind 2011, 2011, s. 3258-3261.

Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Konferenceartikel › Forskning › peer review

TY - GEN

T1 - Microfluidics-mediated isothermal detection of enzyme activity at the single molecule level

AU - Juul, Sissel

AU - Ho, Yi-Ping

AU - Stougaard, Magnus

AU - Koch, Jørn

AU - Andersen, Felicie F

AU - Leong, Kam W

AU - Knudsen, Birgitta R

PY - 2011

Y1 - 2011

N2 - Conventional analysis of enzymatic activity, often carried out on pools of cells, is blind to heterogeneity in the population. Here, we combine microfluidics with a previously developed isothermal rolling circle amplification-based assay to investigate multiple enzymatic activities in down to single cells. This microfluidics-meditated assay performs at very high sensitivity in picoliter incubators with small quantities of biological materials. Furthermore, we demonstrate the assay's capability of multiplexed detection of at least three enzyme activities at the single molecule level.

AB - Conventional analysis of enzymatic activity, often carried out on pools of cells, is blind to heterogeneity in the population. Here, we combine microfluidics with a previously developed isothermal rolling circle amplification-based assay to investigate multiple enzymatic activities in down to single cells. This microfluidics-meditated assay performs at very high sensitivity in picoliter incubators with small quantities of biological materials. Furthermore, we demonstrate the assay's capability of multiplexed detection of at least three enzyme activities at the single molecule level.

KW - Cell Line

KW - Enzymes

KW - Humans

KW - Microfluidics

U2 - 10.1109/IEMBS.2011.6090885

DO - 10.1109/IEMBS.2011.6090885

M3 - Conference article

C2 - 22255034

SN - 2375-7477

VL - 2011

SP - 3258

EP - 3261

JO - I E E E Engineering in Medicine and Biology Society. Conference Proceedings

JF - I E E E Engineering in Medicine and Biology Society. Conference Proceedings

T2 - 2011 Annual International Conference of the IEEE

Y2 - 30 August 2011 through 3 September 2011

ER -

Microfluidics-mediated isothermal detection of enzyme activity at the single molecule level

Abstract

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