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Microfluidics and the quantification of biomolecular interactions
Publikation: Bidrag til tidsskrift/Konferencebidrag i tidsskrift /Bidrag til avis › Tidsskriftartikel › Forskning › peer review
Dokumenter
- 1-s2.0-S0959440X21000294-main
Forlagets udgivne version, 1,63 MB, PDF-dokument
DOI
- https://doi.org/10.1016/j.sbi.2021.02.006
Forlagets udgivne version
- Daniel E Otzen
- Alexander K. Buell, Danmarks Tekniske Universitet ,
- Henrik Jensen, Fida Biosystems Aps
Microfluidic systems under laminar flow conditions provide in-solution information about species size and binding affinities at very modest sample costs. Flow-induced dispersion analysis directly measures the spread of the analyte profile using Taylor dispersion analysis, whereas microfluidic diffusional sizing quantifies the transfer of analyte from one phase to another. Species of sizes between 0.5 and 1000 nm can be analyzed, and different populations resolved. Both techniques also allow analysis in complex media and medium throughput analysis. These properties make them valuable complements to existing approaches to measure biomolecular interactions.
| Originalsprog | Engelsk |
|---|---|
| Tidsskrift | Current Opinion in Structural Biology |
| Vol/bind | 70 |
| Sider (fra-til) | 8-15 |
| Antal sider | 8 |
| ISSN | 0959-440X |
| DOI | |
| Status | Udgivet - okt. 2021 |
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