Aarhus Universitets segl

MFG-E8 as a marker for apoptotic, stressed and activated cells

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Milk fat globule-epidermal growth factor-factor 8 (MFG-E8)/lactadherin's ability to specifically recognize phosphatidylserine (PS) in membranes has been recognized as an excellent tool in a variety of scientific and clinical contexts. An asymmetric pattern of phospholipids across cellular membranes in eukaryotes is a fundamental property in maintaining normal cell function. However, randomization of phospholipids is an equally important event when cells are activated leading to exposure of the otherwise hidden PS crucial in orchestrating downstream events in apoptosis and coagulation. Lactadherin has in recent years been recognized as a sensitive PS binding protein for visualizing apoptosis and as an anticoagulant. Compared to the benchmark PS-probe, annexin V, lactadherin seems to be superior in several PS binding properties. Numerous studies show the usefulness of lactadherin in monitoring cell health in vitro and in vivo, in detecting cell-derived PS exposing microparticles, or for exploring mechanisms in apoptosis. Moreover, radio-labeled lactadherin has been proposed as a non-invasive marker in the clinic for imaging of apoptotic events. Lactadherins PS recognition owes to the proteins C-domains, and has been used in recombinant exosome engineering in addressing proteins of interest to surfaces of nano-membrane particles. This chapter outlines the use of lactadherin as a PS binding protein, based on several publications where many of these are conducted in collaboration with us, and reflects our experimental experiences with the protein over several years.

OriginalsprogEngelsk
TitelMFG-E8 and Inflammation
Antal sider22
ForlagSpringer
Udgivelsesår1 feb. 2014
Sider33-54
ISBN (trykt)9401787646, 9789401787642
ISBN (Elektronisk)9789401787659
DOI
StatusUdgivet - 1 feb. 2014

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