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Mesencephalic dopamine neurons interfacing the shell of nucleus accumbens and the dorsolateral striatum in the rat

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  • jnr.24242

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DOI

  • Floris G Wouterlood, Department of Anatomy & Neurosciences, VU University Medical Center, Neuroscience Campus Amsterdam, 1007 MB Amsterdam, The Netherlands.
  • ,
  • Angela Engel, Department of Anatomy & Neurosciences, VU University Medical Center, Neuroscience Campus Amsterdam, 1007 MB Amsterdam, The Netherlands.
  • ,
  • Mariah Daal, Department of Anatomy & Neurosciences, VU University Medical Center, Neuroscience Campus Amsterdam, 1007 MB Amsterdam, The Netherlands.
  • ,
  • Gertjan Houwen, Department of Anatomy & Neurosciences, VU University Medical Center, Neuroscience Campus Amsterdam, 1007 MB Amsterdam, The Netherlands.
  • ,
  • Aileen Meinderts, Department of Anatomy & Neurosciences, VU University Medical Center, Neuroscience Campus Amsterdam, 1007 MB Amsterdam, The Netherlands.
  • ,
  • Tomàs Jordà Siquier, Department of Anatomy & Neurosciences, VU University Medical Center, Neuroscience Campus Amsterdam, 1007 MB Amsterdam, The Netherlands.
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  • Jeroen A M Beliën, Department of Pathology, VU University Medical Center, Neuroscience Campus Amsterdam, 1007 MB, Amsterdam, The Netherlands.
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  • Yvette C van Dongen, Institut National de la Santé et de la Recherche Médicale, U114, Chaire de Neuropharmacologie, Collège de France, 11 Place Marcelin Berthelot, 75231 Paris Cedex 05, France.
  • ,
  • Jørgen Scheel-Krüger
  • Anne-Marie Thierry, Institut National de la Santé et de la Recherche Médicale, U114, Chaire de Neuropharmacologie, Collège de France, 11 Place Marcelin Berthelot, 75231 Paris Cedex 05, France.
  • ,
  • Henk J Groenewegen, Department of Anatomy & Neurosciences, VU University Medical Center, Neuroscience Campus Amsterdam, 1007 MB Amsterdam, The Netherlands.
  • ,
  • Jean-Michel Deniau, Institut National de la Santé et de la Recherche Médicale, U114, Chaire de Neuropharmacologie, Collège de France, 11 Place Marcelin Berthelot, 75231 Paris Cedex 05, France.

Parallel corticostriatonigral circuits have been proposed that separately process motor, cognitive, and emotional-motivational information. Functional integration requires that interactions exist between neurons participating in these circuits. This makes it imperative to study the complex anatomical substrate underlying corticostriatonigral circuits. It has previously been proposed that dopaminergic neurons in the ventral mesencephalon may play a role in this circuit interaction. Therefore, we studied in rats convergence of basal ganglia circuits by depositing an anterograde neuroanatomical tracer into the ventral striatum together with a retrograde fluorescent tracer ipsilaterally in the dorsolateral striatum. In the mesencephalon, using confocal microscopy, we looked for possible appositions of anterogradely labeled fibers and retrogradely labeled neurons, "enhancing" the latter via intracellular injection of Lucifer Yellow. Tyrosine hydroxylase (TH) immunofluorescence served to identify dopaminergic neurons. In neurophysiological experiments, we combined orthodromic stimulation in the medial ventral striatum with recording from ventral mesencephalic neurons characterized by antidromic stimulation from the dorsal striatum. We observed terminal fields of anterogradely labeled fibers that overlap populations of retrogradely labeled nigrostriatal cell bodies in the substantia nigra pars compacta and lateral ventral tegmental area (VTA), with numerous close appositions between boutons of anterogradely labeled fibers and nigrostriatal, TH-immunopositive neurons. Neurophysiological stimulation in the medial ventral striatum caused inhibition of dopaminergic nigrostriatal neurons projecting to the ventrolateral striatal territory. Responding nigrostriatal neurons were located in the medial substantia nigra and adjacent VTA. Our results strongly suggest a functional link between ventromedial, emotional-motivational striatum, and the sensorimotor dorsal striatum via dopaminergic nigrostriatal neurons.

OriginalsprogEngelsk
TidsskriftJournal of Neuroscience Research
Vol/bind96
Nummer9
Sider (fra-til)1518-1542
Antal sider25
ISSN0360-4012
DOI
StatusUdgivet - sep. 2018

Bibliografisk note

© 2018 The Authors Journal of Neuroscience Research Published by Wiley Periodicals, Inc.

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