Membrane mimetic systems in CryoEM: keeping membrane proteins in their native environment

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DOI

  • Henriette E. Autzen
  • David Julius, University of California, San Francisco
  • ,
  • Yifan Cheng, University of California, San Francisco

Advances in electron microscopes, detectors and data processing algorithms have greatly facilitated the structural determination of many challenging integral membrane proteins that have been evasive to crystallization. These breakthroughs facilitate the application and development of various membrane protein solubilization approaches for structural studies, including reconstitution into lipid nanoparticles. In this review, we discuss various approaches for preparing transmembrane proteins for structural determination with single-particle electron cryo microscopy (cryoEM).

OriginalsprogEngelsk
TidsskriftCurrent Opinion in Structural Biology
Vol/bind58
Sider (fra-til)259-268
Antal sider10
ISSN0959-440X
DOI
StatusUdgivet - okt. 2019

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